Direct and Indirect Reduction of Cr(VI) by Fermentative Fe(III)-Reducing Cellulomonas sp. Strain Cellu-2a
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Hexavalent chromium (Cr(VI)) is recognized to be carcinogenic and toxic and registered as a contaminant in many drinking water regulations. It occurs naturally and is also produced by industrial processes. The reduction of Cr(VI) to Cr(III) has been a central topic for chromium remediation since Cr(III) is less toxic and less mobile. In this study, fermentative Fe(III)-reducing bacterial strains (Cellu-2a, Cellu-5a, and Cellu-5b) were isolated from a groundwater sample and were phylogenetically related to species of Cellulomonas by 16S rRNA gene analysis. One selected strain, Cellu-2a showed its capacity of reduction of both soluble iron (ferric citrate) and solid iron (hydrous ferric oxide, HFO), as well as aqueous Cr(VI). The strain Cellu-2a was able to reduce 15 μM Cr(VI) directly with glucose or sucrose as a sole carbon source under the anaerobic condition and indirectly with one of the substrates and HFO in the same incubations. The heterogeneous reduction of Cr(VI) by the surface-associated reduced iron from HFO by Cellu-2a likely assisted the Cr(VI) reduction. Fermentative features such as large-scale cell growth may impose advantages on the application of bacterial Cr(VI) reduction over anaerobic respiratory reduction.
Abstract
Hexavalent chromium (Cr(VI)) is recognized to be carcinogenic and toxic and registered as a contaminant in many drinking water regulations. It occurs naturally and is also produced by industrial processes. The reduction of Cr(VI) to Cr(III) has been a central topic for chromium remediation since Cr(III) is less toxic and less mobile. In this study, fermentative Fe(III)-reducing bacterial strains (Cellu-2a, Cellu-5a, and Cellu-5b) were isolated from a groundwater sample and were phylogenetically related to species of Cellulomonas by 16S rRNA gene analysis. One selected strain, Cellu-2a showed its capacity of reduction of both soluble iron (ferric citrate) and solid iron (hydrous ferric oxide, HFO), as well as aqueous Cr(VI). The strain Cellu-2a was able to reduce 15 μM Cr(VI) directly with glucose or sucrose as a sole carbon source under the anaerobic condition and indirectly with one of the substrates and HFO in the same incubations. The heterogeneous reduction of Cr(VI) by the surface-associated reduced iron from HFO by Cellu-2a likely assisted the Cr(VI) reduction. Fermentative features such as large-scale cell growth may impose advantages on the application of bacterial Cr(VI) reduction over anaerobic respiratory reduction.
참고문헌 (34)
- Ray RR, "Adverse hematological effects of hexavalent chromium : an overview", Interdiscip. Toxicol, 9, pp.55~65 (2016)
- Halasova E, "Human lung cancer and hexavalent chromium exposure", Neuro Endocrinol. Lett, 30(Suppl 1), pp.182~185 (2009)
- Saha R, "Sources and toxicity of Hexavalent chromium", J. Coord. Chem, 64, pp.1782~1806 (2011)
- Zhang H-K, "Cr(VI) reduction and Cr(III) immobilization by Acinetobacter sp. HK-1 with the assistance of a novel quinone", graphene oxide composite, 2014, pp.48, 12876~12885
- Sani RK, "Dissimilatory reduction of Cr(VI), Fe(III), and U(VI)by Cellulomonas isolates", Appl. Microbiol. Biotechnol, 60, pp.192~199 (2002)
- Viamajala S, "Isolation and characterization of Cr(VI) reducing Cellulomonas spp. from subsurface soils: Implications for long-term chromate reduction", Bioresour. Technol, 98, pp.612~622 (2007)
- Ahmad WA, "Bacterial reduction of Cr(VI) : Operational challenges and feasibility", Curr. Pollut. Rep, 7, pp.115~127 (2021)
- Gu WZ, "Integrative effect of citrate on Cr(VI)and total Cr removal using a sulfate-reducing bacteria consortium", Chemosphere, 279, pp.130437~ (2021)
- Kulp TR, "Dissimilatory arsenate and sulfate reduction in sediments of two hypersaline, arsenic-rich soda lakes : Mono and Searles lakes, California", Appl. Environ. Microbiol, 72, pp.6514~6526 (2006)
- Fletcher KE, "U(VI)reduction to mononuclear U(IV)by Desulfitobacterium species", Environ. Sci. Technol, 44, pp.4705~4709 (2010)
- Liu C, "Reduction kinetics of Fe(III), Co(III), U(VI), Cr(VI), and Tc(VII)in cultures of dissimilatory metal-reducing bacteria", Biotechnol. Bioeng, 80, pp.637~649 (2002)
- List C, "Impact of iron reduction on the metabolism of Clostridium acetobutylicum", Environ. Microbiol, 21, pp.3548~3563 (2019)
- Gerlach R, "Influence of carbon sources and electron shuttles on ferric iron reduction by Cellulomonas sp strain ES6", Biodegradation, 22, pp.983~995 (2011)
- Jin Q, "Energy conservation of anaerobic respiration", Am. J. Sci, 312, pp.573~628 (2012)
- Dalla Vecchia E, "Fe(III)reduction during pyruvate fermentation by Desulfotomaculum reducens strain MI-1", Geobiology, 12, pp.48~61 (2014)
- Ehrenreich A, "Anaerobic oxidation ferrous iron by purple bacteria, a new type of phototrophic metabolism", Appl. Environ. Microbiol, 60, pp.4517~4526 (1994)
- Hegler F, "Physiology of phototrophic iron(II)-oxidizing bacteria : implications for modern and ancient environments", FEMS Microbiol. Ecol, 66, pp.250~260 (2008)
- Widdel F., "Anaerober Abbau von Fettsäuren und Benzoesäure durch neu isolierte Arten sulfat-reduzierender Bakterien", pp.1980
- Tschech A, "Growth yield increase linked to caffeate reduction in Acetobacterium woodii", Arch. Microbiol, 137, pp.163~167 (1984)
- Widdel F, "Studies on dissimilatory sulfate-reducing bacteria that decompose fatty acids. 1. Isolation of new sulfatereducing bacteria enriched with acetate from saline environments. Description of Desulfobacter postgatei gen. nov., sp. nov", Arch. Microbiol, 129, pp.395~400 (1981)
- Altschul SF, "Basic local alignment search tool", J. Mol. Biol, 215, pp.403~410 (1990)
- Quast C, "The SILVA ribosomal RNA gene database project : improved data processing and web-based tools", Nucl. Acids Res, 41, pp.D590~596 (2013)
- Ludwig W, "ARB : a software environment for sequence data", Nucl. Acids Res, 32, pp.1363~1371 (2004)
- Saitou N, "The neighbor-joining method : a new method for reconstructing phylogenetic trees", Mol. Biol. Evol, 4, pp.406~425 (1987)
- Stookey LL, "Ferrozine-a new spectrophotometric reagent for iron Anal", Chem, 42, pp.779~781 (1970)
- Rangaswamy S, "Screening of facultative anaerobic bacteria utilizing D-xylose for xylitol production", Appl. Microbiol. Biotechnol, 60, pp.88~93 (2002)
- Poulsen HV, "Aerobic and anaerobic cellulase production by Cellulomonas uda", Arch. Microbiol, 198, pp.725~735 (2016)
- Dobbin PS, "Dissimilatory Fe(III)reduction by Clostridium beijerinckii isolated from freshwater sediment using Fe(III)maltol enrichment", FEMS Microbiol. Lett, 176, pp.131~138 (1999)
- Lovley DR, "Organic matter mineralization with the reduction of ferric iron : a review", Geomicrobiol. J, 5, pp.375~399 (1987)
- Scala DJ, "Characterization of Fe(III)-reducing enrichment cultures and isolation of Fe(III)-reducing bacteria from the Savannah River site, South Carolina", Res. Microbiol, 157, pp.772~783 (2006)
- Zhang LM, "Iron reduction by diverse actinobacteria under oxic and pH-neutral conditions and the formation of secondary minerals", Chem. Geol, 525, pp.390~399 (2019)
- Viamajala S, "Toxic effects of chromium(VI)on anaerobic and aerobic growth of Shewanella oneidensis MR-1", Biotechnol. Prog, 20, pp.87~95 (2004)
- Peretyazhko T, "Reduction of Tc(VII)by Fe(II)sorbed on Al(hydr)oxides", Environ. Sci. Technol, 42, pp.5499~5506 (2008)
- Charlet L, "Natural attenuation of TCE, As, Hg linked to the heterogeneous oxidation of Fe(II) : an AFM study", Chem. Geol, 190, pp.303~319 (2002)