Protective Effects of a Novel Lactobacillus brevis Strain with Probiotic Characteristics against Staphylococcus aureus Lipoteichoic Acid-Induced Intestinal Inflammatory Response
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Probiotics can effectively modulate host immune responses and prevent gastrointestinal diseases. The objective of this study was to investigate the probiotic characteristics of Lactobacillus brevis KU15152 isolated from kimchi and its protective potential against intestinal inflammation induced by Staphylococcus aureus lipoteichoic acid (aLTA). L. brevis KU15152 exhibited a high survival rate in artificial gastric and bile environments. Additionally, the adhesion capability of the strain to HT-29 cells was higher than that of L. rhamnosus GG. L. brevis KU15152 did not produce harmful enzymes, such as β-glucuronidase, indicating that it could be used as a potential probiotic. The antiinflammatory potential of L. brevis KU15152 was determined in HT-29 cells. Treatment with L. brevis KU15152 suppressed the production of interleukin-8 without inducing significant cytotoxicity. The downregulatory effects of L. brevis KU15152 were involved in the suppression of nuclear factorkappa B activation mediated by the extracellular signal-regulated kinase and Akt signaling pathways. Collectively, these data suggest that L. brevis KU15152 can be used in developing therapeutic and prophylactic products to manage and treat aLTA-induced intestinal damage.
Abstract
Probiotics can effectively modulate host immune responses and prevent gastrointestinal diseases. The objective of this study was to investigate the probiotic characteristics of Lactobacillus brevis KU15152 isolated from kimchi and its protective potential against intestinal inflammation induced by Staphylococcus aureus lipoteichoic acid (aLTA). L. brevis KU15152 exhibited a high survival rate in artificial gastric and bile environments. Additionally, the adhesion capability of the strain to HT-29 cells was higher than that of L. rhamnosus GG. L. brevis KU15152 did not produce harmful enzymes, such as β-glucuronidase, indicating that it could be used as a potential probiotic. The antiinflammatory potential of L. brevis KU15152 was determined in HT-29 cells. Treatment with L. brevis KU15152 suppressed the production of interleukin-8 without inducing significant cytotoxicity. The downregulatory effects of L. brevis KU15152 were involved in the suppression of nuclear factorkappa B activation mediated by the extracellular signal-regulated kinase and Akt signaling pathways. Collectively, these data suggest that L. brevis KU15152 can be used in developing therapeutic and prophylactic products to manage and treat aLTA-induced intestinal damage.
참고문헌 (37)
- Conlon MA, "The impact of diet and lifestyle on gut microbiota and human health", Nutrients, 7, pp.17~44 (2015)
- Mendes V, "Mechanisms by which the gut microbiota influences cytokine production and modulates host inflammatory responses", J. Interferon Cytokine Res, 39, pp.393~409 (2019)
- Issac Ginsburg, "Role of lipoteichoic acid in infection and inflammation", Lancet Infect. Dis, 2, pp.171~179 (2002)
- Jeong JH, "Differential immunue-stimulatory effects of LTAs from different lactic acid bacteria via MAPK signaling pathway in RAW 264.7 cells", Immunobiol, 220, pp.460~466 (2015)
- Shokryazdan P, "Probiotic potential of Lactobacillus strains with antimicrobial activity against some human pathogenic strains", Biomed. Res. Int, 2014, pp.927268~927216 (2014)
- Jiang Y, "Induction of cytokines via NF-κB and p38 MAP kinase signaling pathways associated with the immunomodulation by Lactobacillus plantarum NDC 75017 in vitro and in vivo", J. Func. Foods, 20, pp.215~225 (2016)
- T. Gupta, "Lactobacillus fermentum (MTCC-5898) alleviates Escherichia coli-induced inflammatory responses in intestinal epithelial cells by modulating immune genes and NF-κB signaling", Journal of Applied Microbiology, 131(6), pp.3008~3017 (2021), Oxford University Press (OUP)[DOI]
- Li X, "Protective effects of a novel Lactobacillus rhamnosus strain with probiotic characteristics against lipopolysaccharide-induced intestinal inflammation in vitro and in vivo", Food Funct, 11, pp.5799~ (2020)
- Song MW, "Probiotic characteristics of Lactobacillus brevis B13-2 isolated from kimchi and investigation of antioxidant and immune-modulating abilities of its heat-killed cells", LWT-Food Sci. Technol, 128, pp.109452~ (2020)
- 유형석, "Antagonistic and antioxidant effect of probiotic Weissella cibaria JW15", Food Science and Biotechnology, 28(3), pp.851~855 (2019), 한국식품과학회[DOI]
- 김기태, "Probiotic properties of novel probiotic Levilactobacillus brevis KU15147 isolated from radish kimchi and its antioxidant and immune-enhancing activities", Food Science and Biotechnology, 30(2), pp.257~265 (2021), 한국식품과학회[DOI]
- 유형석, "Anti-Inflammatory Potential of Probiotic Strain Weissella cibaria JW15 Isolated from Kimchi through Regulation of NF-κB and MAPKs Pathways in LPS-Induced RAW 264.7 Cells", Journal of Microbiology and Biotechnology, 29(7), pp.1022~1032 (2019), 한국미생물·생명공학회[DOI]
- 김한근, "Inhibitory Effect of Lactobacillus plantarum Extracts on HT-29 Colon Cancer Cell Apoptosis Induced by Staphylococcus aureus and Its Alpha-Toxin", Journal of Microbiology and Biotechnology, 25(11), pp.1849~1855 (2015), 한국미생물·생명공학회[DOI]
- Li SC, "Combination of Lactobacillus acidophilus and Bifidobacterium animalis subsp. lactis shows a stronger anti-inflammatory effect than individual strains in HT-29 cells", Nutrients, 11, pp.969~ (2019)
- 장혜지, "Probiotic characterization of Lactobacillus brevis KU15153 showing antimicrobial and antioxidant effect isolated from kimchi", Food Science and Biotechnology, 28(5), pp.1521~1528 (2019), 한국식품과학회[DOI]
- Yu HS, "Evaluation of the probiotic characteristics and prophylactic potential of Weissella cibaria strains isolated from kimchi", LWT-Food Sci. Technol, 112, pp.108229~ (2019)
- Kim WJ, "Chrysanthemum indicum suppresses adipogenesis by inhibiting mitotic clonal expansion in 3T3-L1 preadipocytes", J. Food Biochem, 45, pp.e13896~ (2021)
- Yu HS, "Inula britannica inhibits adipogenesis of 3T3-L1 preadipocytes via modulation of mitotic clonal expansion involving ERK 1", 2 and Akt signaling pathways, 2020, pp.12, 3037~
- Yang SJ, "Probiotic properties and antioxidant activities of Pediococcus pentosaceus SC28 and Levilactobacillus brevis KU15151 in fermented black gamju", Foods, 9, pp.1154~ (2020)
- Singh S, "Probiotic attributes and prevention of LPS-induced proinflammatory stress in RAW264.7 macrophages and human intestinal epithelial cell line (Caco-2) by newly isolated Weissella cibaria strains", Food Func, 9, pp.1254~ (2018)
- Bao Y, "Screening of potential probiotic properties of Lactobacillus fermentum isolated from traditional dairy products", Food Control, 21, pp.695~701 (2010)
- Guo CF, "Comparison of lactobacilli isolated from Chinese suan-tsai and koumiss for their probiotic and functional properties", J. Funct. Foods, 12, pp.294~302 (2015)
- Argyri AA, "Selection of potential probiotic lactic acid bacteria from fermented olives by in vitro tests", Food Microbiol, 33, pp.282~291 (2013)
- Lee CS, "Anti-inflammatory and anti-osteoporotic potential of Lactobacillus plantarum A41 and L", Probiotics Antimicrob, 12, pp.623~634 (2020)
- 김한근, "Inhibitory Effects of Lactobacillus plantarum Lipoteichoic Acid (LTA) onStaphylococcus aureus LTA-Induced Tumor Necrosis Factor-Alpha Production", Journal of Microbiology and Biotechnology, 18(6), pp.1191~1196 (2008), 한국미생물·생명공학회
- Jinbo Wang, "Different effects of lipoteichoic acid from C. butyricum and S. aureus on inflammatory responses of HT-29 cells", International Journal of Biological Macromolecules, 87, pp.481~487 (2016), Elsevier BV[DOI]
- 김한근, "Lactobacillus plantarum Lipoteichoic Acid Alleviates TNF-alpha-Induced Inflammation in the HT-29 Intestinal Epithelial Cell Line", Molecules and Cells, 33(5), pp.479~486 (2012), 한국분자세포생물학회[DOI]
- Singh S, "CXCL8 and its cognate receptors in melanoma progression and metastasis", Future Oncol, 6, pp.111~116 (2010)
- Hubert-Buron A, "Glutamine pretreatment reduces IL-8 production in human intestinal epithelial cells by limiting IκBα ubiquitination", J. Nutr, 136, pp.1461~1465 (2006)
- Buchholz KR, "The cytosolic pattern recognition receptor NOD1 induces inflammatory interleukin-8 during Chlamydia trachomatis infection", Infect. Immun, 76, pp.3150~3155 (2008)
- Fernandes AF, "Proteasome inactivation promotes p38 mitogen-activated protein kinase-dependent phosphatidylinositol 3-kinase activation and increases interleukin-8 production in retinal pigment epithelial cells", Mol. Biol. Cell, 16, pp.3690~3699 (2009)
- Zhu L, "Qing Chang Hua Shi granule ameliorate inflammation in experimental rats and cell model of ulcerative colitis through MEK", ERK signaling pathway, 2019, pp.116, 108967~
- Kohno M, "Targeting the extracellular signal-regulated kinase pathway in cancer therapy", Biol. Pharm. Bull, 12, pp.1781~1784 (2011)
- Long X, "IL-8, a novel messenger to cross-link inflammation and tumor EMT via autocrine and paracrine pathways(review)", Int. J. Oncol, 48, pp.5~12 (2016)
- Son YO, "Reactive oxygen species mediate Cr(VI)-induced carcinogenesis through PI3K", AKT-dependent activation of GSK-3β, Toxicol. Appl. Pharmacol, pp.2013, 271[DOI]
- Maurer U, "GSK-3-at the crossroads of cell death and survival", J. Cell. Sci, 127, pp.1369~1378 (2014)
- Zhang Y, "Neuroprotective effect of ginsenoside Rg1 prevents cognitive impairment induced by isoflurane anesthesia in aged rats via antioxidant, anti-inflammatory and anti-apoptotic effects mediated by the PI3K", AKT, Mol. Med. Rep, pp.2016, 14[DOI]
