Bulb of Lilium longiflorum Thunb Extract Fermented with Lactobacillus acidophilus Reduces Inflammation in a Chronic Obstructive Pulmonary Disease Model
0
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Chronic obstructive pulmonary disease (COPD), one of the leading causes of death worldwide, is caused by repeated exposure to harmful matter, such as cigarette smoke. Although Lilium longiflorum Thunb (LLT) has anti-inflammatory effects, there is no report on the fermented LLT bulb extract regulating lung inflammation in COPD. Thus, we investigated the protective effect of LLT bulb extract fermented with Lactobacillus acidophilus 803 in COPD mouse models induced by cigarette smoke extract (CSE) and porcine pancreas elastase (PPE). Oral administration of the fermented product (LS803) suppressed the production of inflammatory mediators and the infiltration of immune cells involving neutrophils and macrophages, resulting in protective effects against lung damage. In addition, LS803 inhibited CSE- and LPS-induced IL-6 and IL-8 production in airway epithelial H292 cells as well as suppressed PMA-induced formation of neutrophil extracellular traps in HL-60 cells. In particular, LS803 significantly repressed the elevated IL-6 and MIP-2 production after CSE and LPS stimulation by suppressing the activity of the nuclear factor kappa-light-chainenhancer of activated B (NFκB) in mouse peritoneal macrophages. Therefore, our results suggest that the fermented product LS803 is effective in preventing and alleviating lung inflammation.
Abstract
Chronic obstructive pulmonary disease (COPD), one of the leading causes of death worldwide, is caused by repeated exposure to harmful matter, such as cigarette smoke. Although Lilium longiflorum Thunb (LLT) has anti-inflammatory effects, there is no report on the fermented LLT bulb extract regulating lung inflammation in COPD. Thus, we investigated the protective effect of LLT bulb extract fermented with Lactobacillus acidophilus 803 in COPD mouse models induced by cigarette smoke extract (CSE) and porcine pancreas elastase (PPE). Oral administration of the fermented product (LS803) suppressed the production of inflammatory mediators and the infiltration of immune cells involving neutrophils and macrophages, resulting in protective effects against lung damage. In addition, LS803 inhibited CSE- and LPS-induced IL-6 and IL-8 production in airway epithelial H292 cells as well as suppressed PMA-induced formation of neutrophil extracellular traps in HL-60 cells. In particular, LS803 significantly repressed the elevated IL-6 and MIP-2 production after CSE and LPS stimulation by suppressing the activity of the nuclear factor kappa-light-chainenhancer of activated B (NFκB) in mouse peritoneal macrophages. Therefore, our results suggest that the fermented product LS803 is effective in preventing and alleviating lung inflammation.
참고문헌 (41)
- Rabe KF, "Chronic obstructive pulmonary disease", Lancet, 389, pp.1931~1940 (2017)
- Buist AS, "International variation in the prevalence of COPD (the BOLD Study): a population-based prevalence study", Lancet, 370, pp.741~750 (2007)
- Salvi SS, "Chronic obstructive pulmonary disease in non-smokers", Lancet, 374, pp.733~743 (2009)
- Bracke KR, "Cigarette smoke-induced pulmonary inflammation and emphysema are attenuated in CCR6-deficient mice", J. Immunol, 177, pp.4350~4359 (2006)
- Barnes PJ, "Inflammatory mechanisms in patients with chronic obstructive pulmonary disease", J. Allergy Clin. Immunol, 138, pp.16~27 (2016)
- Barnes PJ, "Alveolar macrophages as orchestrators of COPD", COPD, 1, pp.59~70 (2004)
- Rinaldi M, "Long-term nose-only cigarette smoke exposure induces emphysema and mild skeletal muscle dysfunction in mice", Dis. Model Mech, 5, pp.333~341 (2012)
- Barnes PJ, "Chronic obstructive pulmonary disease: molecular and cellular mechanisms", Eur. Respir. J, 22, pp.672~688 (2003)
- Foronjy R, "The role of collagenase in emphysema", Respir. Res, 2, pp.348~352 (2001)
- Papi A, "Pathophysiology of exacerbations of chronic obstructive pulmonary disease", Proc. Am. Thorac. Soc, 3, pp.245~251 (2006)
- Fernandez-Real JM, "Smoking, fat mass and activation of the tumor necrosis factor-alpha pathway", Int. J. Obes. Relat. Metab. Disord, 27, pp.1552~1556 (2003)
- Herold S, "Acute lung injury: how macrophages orchestrate resolution of inflammation and tissue repair", Front. Immunol, 2, pp.65~ (2011)
- Tetley TD, "Macrophages and the pathogenesis of COPD", Chest, 121(5 Suppl), pp.156S~159S (2002)
- Brinkmann V, "Neutrophil extracellular traps kill bacteria", Science, 303, pp.1532~1535 (2004)
- Deshmukh HS, "Matrix metalloproteinase-14 mediates a phenotypic shift in the airways to increase mucin production", Am. J. Respir. Crit. Care Med, 180, pp.834~845 (2009)
- Munafo JP Jr, "Isolation and structural determination of steroidal glycosides from the bulbs of easter lily (Lilium longiflorum Thunb.)", J. Agric. Food Chem, 58, pp.8806~8813 (2010)
- Kwon OK, "Anti-inflammatory effects of methanol extracts of the root of Lilium lancifolium on LPS-stimulated Raw264.7 cells", J. Ethnopharmacol, 130, pp.28~34 (2010)
- Ma T, "Bioassay-guided isolation of anti-inflammatory components from the bulbs of Lilium brownii var. viridulum and identifying the underlying mechanism through acting on the NF-κB", MAPKs pathway, 2017, pp.22, 506~
- Jin L, "Phenolic compounds and antioxidant activity of bulb extracts of six lilium species native to China", Molecules, 17, pp.9361~9378 (2012)
- Zhijie Z, "Pharmacodynamics research of Lilium brownii polysaccharide", Traditional Chinese Drug Research and Clinical Pharmacology (2007)
- Luo J, "Preparative separation of phenylpropenoid glycerides from the bulbs of Lilium lancifolium by high-speed counter-current chromatography and evaluation of their antioxidant activities", Food Chem, 131, pp.1056~1062 (2012)
- Munafo JP Jr, "Quantitative analysis of phenylpropanoid glycerol glucosides in different organs of easter lily (Lilium longiflorum Thunb.)", J. Agric. Food Chem, 63, pp.4836~4842 (2015)
- Lee E, "Lilium lancifolium Thunb. extract attenuates pulmonary inflammation and air space enlargement in a cigarette smoke-exposed mouse model", J. Ethnopharmacol, 149, pp.148~156 (2013)
- Jung SY, "Epilobiumpyrricholophum extract suppresses porcine pancreatic elastase and cigarette smoke extract-induced inflammatory response in a chronic obstructive pulmonary disease model", Foods, 10, pp.2929~ (2021)
- Shin DU, "Camellia sinensis L. alleviates pulmonary inflammation induced by porcine pancreas elastase and cigarette smoke extract", Antioxidants (Basel), 11, pp.1683~ (2022)
- Kim SY, "Artemisia gmelinii attenuates lung inflammation by suppressing the NF-κB", MAPK pathway, 2022, pp.11, 568~
- Christenson SA, "Chronic obstructive pulmonary disease", Lancet, 399, pp.2227~2242 (2022)
- Hüls A, "Ambient particulate matter and COPD in China: a challenge for respiratory health research", Thorax, 72, pp.771~772 (2017)
- Savale L, "Shortened telomeres in circulating leukocytes of patients with chronic obstructive pulmonary disease", Am. J. Respir. Crit. Care Med, 179, pp.566~571 (2009)
- Zosky GR, "Vitamin D deficiency causes deficits in lung function and alters lung structure", Am. J. Respir. Crit. Care Med, 183, pp.1336~1343 (2011)
- Martindale S, "Antioxidant intake in pregnancy in relation to wheeze and eczema in the first two years of life", Am. J. Respir. Crit. Care Med, 171, pp.121~128 (2005)
- Bagdonas E, "Novel aspects of pathogenesis and regeneration mechanisms in COPD", Int. J. Chron. Obstruct. Pulmon. Dis, 10, pp.995~1013 (2015)
- Shrine N, "New genetic signals for lung function highlight pathways and chronic obstructive pulmonary disease associations across multiple ancestries", Nat. Genet, 51, pp.481~493 (2019)
- Ortega VE, "The Effects of rare SERPINA1 variants on lung function and emphysema in SPIROMICS", Am. J. Respir. Crit. Care Med, 201, pp.540~554 (2020)
- Zuo L, "Interrelated role of cigarette smoking, oxidative stress, and immune response in COPD and corresponding treatments", Am. J. Physiol. Lung Cell Mol. Physiol, 307, pp.L205~L218 (2014)
- Brody JS, "State of the art. Chronic obstructive pulmonary disease, inflammation, and lung cancer", Proc. Am. Thorac. Soc, 3, pp.535~537 (2006)
- Gharib SA, "Matrix metalloproteinases in emphysema", Matrix Biol, 73, pp.34~51 (2018)
- Belchamber KBR, "Defective bacterial phagocytosis is associated with dysfunctional mitochondria in COPD macrophages", Eur. Respir. J, 54, pp.1802244~ (2019)
- Polosukhin VV, "Secretory IgA deficiency in individual small airways is associated with persistent inflammation and remodeling", Am. J. Respir. Crit. Care Med, 195, pp.1010~1021 (2017)
- Caramori G, "Role of transcription factors in the pathogenesis of asthma and COPD", Cell Commun. Adhes, 20, pp.21~40 (2013)
- Culpitt SV, "Effect of high dose inhaled steroid on cells, cytokines, and proteases in induced sputum in chronic obstructive pulmonary disease", Am. J. Respir. Crit. Care Med, 160(5 Pt1), pp.1635~1639 (1999)