Inoculation Effect of Methanotrophs on Rhizoremediation Performance and Methane Emission in Diesel-Contaminated Soil
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During the rhizoremediation of diesel-contaminated soil, methane (CH4), a representative greenhouse gas, is emitted as a result of anaerobic metabolism of diesel. The application of methantrophs is one of solutions for the mitigation CH4 emissions during the rhizoremediation of diesel-contaminated soil. In this study, CH4-oxidizing rhizobacteria, Methylocystis sp. JHTF4 and Methyloversatilis sp. JHM8, were isolated from rhizosphere soils of tall fescue and maize, respectively. The maximum CH4 oxidation rates for the strains JHTF4 and JHM8 were 65.8 and 33.8 mmol·g-DCW-1·h-1, respectively. The isolates JHTF4 and JHM8 couldn't degrade diesel. The inoculation of the isolate JHTF4 or JHM8 significantly enhanced diesel removal during rhizoremediation of diesel-contaminated soil planted with maize for 63 days. Diesel removal in the tall fescue-planting soil was enhanced by inoculating the isolates until 50 days, while there was no significant difference in removal efficiency regardless of inoculation at day 63. In both the maize and tall fescue planting soils, the CH4 oxidation potentials of the inoculated soils were significantly higher than the potentials of the non-inoculated soils. In addition, the gene copy numbers of pmoA, responsible for CH4 oxidation, in the inoculated soils were significantly higher than those in the non-inoculated soils. The gene copy numbers ratio of pmoA to 16S rDNA (the ratio of methanotrophs to total bacteria) in soil increased during rhizoremediation. These results indicate that the inoculation of Methylocystis sp. JHTF4 and Methyloversatilis sp. JHM8, is a promising strategy to minimize CH4 emissions during the rhizoremediation of diesel-contaminated soil using maize or tall fescue.
Abstract
During the rhizoremediation of diesel-contaminated soil, methane (CH4), a representative greenhouse gas, is emitted as a result of anaerobic metabolism of diesel. The application of methantrophs is one of solutions for the mitigation CH4 emissions during the rhizoremediation of diesel-contaminated soil. In this study, CH4-oxidizing rhizobacteria, Methylocystis sp. JHTF4 and Methyloversatilis sp. JHM8, were isolated from rhizosphere soils of tall fescue and maize, respectively. The maximum CH4 oxidation rates for the strains JHTF4 and JHM8 were 65.8 and 33.8 mmol·g-DCW-1·h-1, respectively. The isolates JHTF4 and JHM8 couldn't degrade diesel. The inoculation of the isolate JHTF4 or JHM8 significantly enhanced diesel removal during rhizoremediation of diesel-contaminated soil planted with maize for 63 days. Diesel removal in the tall fescue-planting soil was enhanced by inoculating the isolates until 50 days, while there was no significant difference in removal efficiency regardless of inoculation at day 63. In both the maize and tall fescue planting soils, the CH4 oxidation potentials of the inoculated soils were significantly higher than the potentials of the non-inoculated soils. In addition, the gene copy numbers of pmoA, responsible for CH4 oxidation, in the inoculated soils were significantly higher than those in the non-inoculated soils. The gene copy numbers ratio of pmoA to 16S rDNA (the ratio of methanotrophs to total bacteria) in soil increased during rhizoremediation. These results indicate that the inoculation of Methylocystis sp. JHTF4 and Methyloversatilis sp. JHM8, is a promising strategy to minimize CH4 emissions during the rhizoremediation of diesel-contaminated soil using maize or tall fescue.
참고문헌 (49)
- Saravanan A, "Rhizoremediation - A promising tool for the removal of soil contaminants: a review", J. Environ. Chem. Eng, 8, pp.103543~ (2020)
- Syranidou E, "Juncus spp.-The helophyte for all (phyto)remediation purposes?", N. Biotechnol, 38, pp.43~55 (2017)
- Gainer A, "Advancing soil ecological risk assessments for petroleum hydrocarbon contaminated soils in Canada: persistence, organic carbon normalization and relevance of species assemblages", Sci. Total Environ, 668, pp.400~410 (2019)
- Park IS, "Determination of a risk management primer at petroleum-contaminated sites: developing new human health risk assessment strategy", J. Hazard. Mater, 185, pp.1374~1380 (2011)
- Baoune H, "Effectiveness of the Zea mays-Streptomyces association for the phytoremediation of petroleum hydrocarbons impacted soils", Ecotoxicol. Environ. Saf, 184, pp.109591~ (2019)
- Lee YY, "Seasonal dynamics of bacterial community structure in diesel oil-contaminated soil cultivated with tall fescue (Festuca arundinacea)", Int. J. Environ. Res. Public Health, 19(8), pp.4629~ (2022)
- 서윤주, "Effects of Plant and Soil Amendment on Remediation Performance and Methane Mitigation in Petroleum-Contaminated Soil", Journal of Microbiology and Biotechnology, 31(1), pp.104~114 (2021), 한국미생물·생명공학회[DOI]
- Shahzad A, "Hydrocarbon degradation in oily sludge by bacterial consortium assisted with alfalfa (Medicago sativa L.) and maize (Zea mays L.)", Arab. J. Geosci, 13, pp.879~ (2020)
- Correa-García S, "Rhizoremediation of petroleum hydrocarbons: a model system for plant microbiome manipulation", Microb. Biotechnol, 11, pp.819~832 (2018)
- Jason-Ogugbue VT, "Uptake and bioaccumulation of diverse hydrocarbon compounds by selected food plants artificially exposed to bioremediated crude oil-contaminated soils", Acta Fytotechn Zootechn, 24, pp.185~201 (2021)
- Yang J, "Increased soil methane emissions and methanogenesis in oil contaminated areas", L. Degrad. Dev, 29, pp.563~571 (2018)
- Climate Change 2014: Synthesis Report, "IPCC", ~ (2014)
- Ming A, "Key messages from the IPCC AR6 climate science report", Cambridge Open Engage. doi:10.33774, 2021
- Semple KT, "Impact of composting strategies on the treatment of soils contaminated with organic pollutants", Environ. Pollut, 112, pp.269~283 (2001)
- Davamani V, "Mitigation of methane gas emissions in flooded paddy soil through the utilization of methanotrophs", Sci. Total Environ, 726, pp.138570~ (2020)
- Lee YY, "Seasonal characteristics of odor and methane mitigation and the bacterial community dynamics in an on-site biocover at a sanitary landfill", Waste Manag, 71, pp.277~286 (2018)
- Reis PCJ, "Rapid shifts in methanotrophic bacterial communities mitigate methane emissions from a tropical hydropower reservoir and its downstream river", Sci. Total Environ, 748, pp.141374~ (2020)
- Ruiz-Ruiz P, "Methanotroph-microalgae co-culture for greenhouse gas mitigation:effect of initial biomass ratio and methane concentration", Chemosphere, 259, pp.127418~ (2020)
- 양효주, "Characterization of the Bacterial Community Associated with Methane and Odor in a Pilot-Scale Landfill Biocover under Moderately Thermophilic Conditions", Journal of Microbiology and Biotechnology, 31(6), pp.803~814 (2021), 한국미생물·생명공학회[DOI]
- Lee YY, "Evaluation of rhizoremediation and methane emission in diesel-contaminated soil cultivated with tall fescue (Festuca arundinacea)", Environ. Res, 194, pp.110606~ (2021)
- Herlemann DP, "Transitions in bacterial communities along the 2000 km salinity gradient of the Baltic Sea", ISME J, 5, pp.1571~1579 (2011)
- 김태관, "Microbial Community Analysis of a Methane-Oxidizing Biofilm Using Ribosomal Tag Pyrosequencing", Journal of Microbiology and Biotechnology, 22(3), pp.360~370 (2012), 한국미생물·생명공학회
- 이은희, "Characterization of Methane Oxidation by a Methanotroph Isolated from a Landfill Cover Soil, South Korea", Journal of Microbiology and Biotechnology, 21(7), pp.753~756 (2011), 한국미생물·생명공학회
- Jäckel U, "Microbial ethylene production and inhibition of methanotrophic activity in a deciduous forest soil", Soil Biol. Biochem, 36, pp.835~840 (2004)
- 이수정, "옥수수와 톨페스큐 근권 유래의 메탄 산화 및 아산화질소 환원 세균 컨소시움 특성", 한국미생물·생명공학회지, 49(2), pp.225~238 (2021), 한국미생물·생명공학회[DOI]
- Kolb S, "Quantitative detection of methanotrophs in soil by Novel", Society, 69, pp.2423~2429 (2003)
- Hanson RS, "Methanotrophic bacteria", Microbiol. Rev, 60, pp.439~471 (1996)
- Abazari M, "Methane removal of isolated Methylocystis strains in the culture medium designed by evaluating strain capacity under adverse donditions", Biol. J. Microorg, 10, pp.23~36 (2021)
- Dunfield PF, "Isolation of a Methylocystis strain containing a novel pmoAlike gene", FEMS Microbiol. Ecol, 41, pp.17~26 (2002)
- Jung GY, "Genomic and physiological properties of a facultative methane-oxidizing bacterial strain of Methylocystis sp. from a wetland", Microorganisms, 8, pp.1~20 (2020)
- Rumah BL, "Isolation and characterisation of Methylocystis spp. for poly-3-hydroxybutyrate production using waste methane feedstocks", AMB Express, 11(1), pp.1~13 (2021)
- Pardi-Comensoli L, "Microbial depolymerization of epoxy resins: a novel approach to a complex challenge", Appl. Sci, 12(1), pp.466~ (2022)
- Shrestha R, "Anaerobic microbial corrosion of carbon steel under conditions relevant for deep geological repository of nuclear waste", Sci. Total. Environ, 800, pp.149539~ (2021)
- Ossai IC, "Remediation of soil and water contaminated with petroleum hydrocarbon: a review", Environ. Technol. Innov, 17, pp.100526~ (2020)
- 서윤주, "유류오염토양 근권정화기술 동향 및 온실가스 배출 특성", 한국미생물·생명공학회지, 48(2), pp.99~112 (2020), 한국미생물·생명공학회[DOI]
- de la Fuente Cantó C, "An extended root phenotype: the rhizosphere, its formation and impacts on plant fitness", Plant J, 103, pp.951~964 (2020)
- 구소연, "중금속 오염 토양의 식품상 복원에 있어 식물과근권세균의 상호작용", 한국미생물·생명공학회지, 34(2), pp.83~93 (2006), 한국미생물·생명공학회
- Vergani L, "Novel PCB-degrading Rhodococcus strains able to promote plant growth for assisted rhizoremediation of historically polluted soils", PLoS One, 14, pp.e0221253~ (2019)
- Iannucci A, "Relationships between root morphology, root exudate compounds and rhizosphere microbial community in durum wheat", Appl. Soil Ecol, 158, pp.103781~ (2021)
- Hong SH, "Rhizoremediation of diesel-contaminated soil using the plant growth-promoting rhizobacterium Gordonia sp. S2RP-17", Biodegradation, 22, pp.593~601 (2011)
- Lee YY, "Dynamics of bacterial functional genes and community structures during rhizoremediation of diesel-contaminated compost-amended soil. J. Environ. Sci. Heal. - Part A Toxic", Hazardous Subst, 2021, pp.56, 1107~1120
- Nie Y, "Diverse alkane hydroxylase genes in microorganisms and environments", Sci. Rep, 4, pp.4968~ (2014)
- Gutierrez T, "Role of methylotrophs in the degradation of hydrocarbons during the deepwater horizon oil spill", ISME J, 8, pp.2543~2545 (2014)
- Chen P, "Cometabolic degradation mechanism and microbial network response of methanotrophic consortia to chlorinated hydrocarbon solvents", Ecotoxicol. Environ. Saf, 230, pp.113110~ (2022)
- Semrau JD, "Bioremediation via methanotrophy: overview of recent findings and suggestions for future research", Front. Microbiol, 2, pp.209~ (2011)
- Serrano-Silva N, "Methanogenesis and methanotrophy in soil: a review", Pedosphere, 24, pp.291~307 (2014)
- Cui J, "Diversity of active root-associated methanotrophs of three emergent plants in a eutrophic wetland in northern China", AMB Express, 10, pp.48~ (2020)
- Degelmann DM, "Different atmospheric methane-oxidizing communities in european beech and norway spruce soils", Appl. Environ. Microbiol, 76, pp.3228~3235 (2010)
- Alam MS, "Inhibition of methane oxidation by nitrogenous fertilizers in a paddy soil", Front. Microbiol, 3, pp.246~ (2012)