Neuroprotective Effects of Heat-Killed Levilactobacillus brevis KU15152 on H2O2- Induced Oxidative Stress
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초록
This study proposed to demonstrate the neuroprotective effects of heat-killed Levilactobacillus brevis KU15152. Heat-killed L. brevis KU15152 showed antioxidant activity similar to that of Lacticaseibacillus rhamnosus GG, in terms of radical scavenging activity. To evaluate the neuroprotective effects, conditioned medium (CM) obtained by incubating heat-killed bacteria in intestinal cells (HT29) was used through gut-brain axis. CM from L. brevis KU15152 protected neuroblastoma cells (SHSY5Y) against H2O2-induced oxidative stress. Pretreatment with CM significantly alleviated the morphological changes induced by H2O2. Heat-killed L. brevis KU15152 showed an increased brainderived neurotrophic factor (BDNF) expression in HT-29 cells. L. brevis KU15152–CM remarkably downregulated the Bax/Bcl-2 ratio, while upregulating the expression of BDNF and tyrosine hydroxylase (TH) in SH-SY5Y cells. Furthermore, L. brevis KU15152–CM reduced caspase-3 activity following H2O2 treatment. In conclusion, L. brevis KU15152 can be potentially used as food materials to avoid neurodegenerative diseases.
Abstract
This study proposed to demonstrate the neuroprotective effects of heat-killed Levilactobacillus brevis KU15152. Heat-killed L. brevis KU15152 showed antioxidant activity similar to that of Lacticaseibacillus rhamnosus GG, in terms of radical scavenging activity. To evaluate the neuroprotective effects, conditioned medium (CM) obtained by incubating heat-killed bacteria in intestinal cells (HT29) was used through gut-brain axis. CM from L. brevis KU15152 protected neuroblastoma cells (SHSY5Y) against H2O2-induced oxidative stress. Pretreatment with CM significantly alleviated the morphological changes induced by H2O2. Heat-killed L. brevis KU15152 showed an increased brainderived neurotrophic factor (BDNF) expression in HT-29 cells. L. brevis KU15152–CM remarkably downregulated the Bax/Bcl-2 ratio, while upregulating the expression of BDNF and tyrosine hydroxylase (TH) in SH-SY5Y cells. Furthermore, L. brevis KU15152–CM reduced caspase-3 activity following H2O2 treatment. In conclusion, L. brevis KU15152 can be potentially used as food materials to avoid neurodegenerative diseases.
참고문헌 (45)
- Tian W, "Neuroprotective effects of Cornus officinalis on stress-induced hippocampal deficits in rats and H2O2-induced neurotoxicity in SH-SY5Y neuroblastoma cells", Antioxidants, 9, pp.27~ (2019)
- Behl C, "Alzheimer's disease and oxidative stress: implications for novel therapeutic approaches", Prog. Neurobiol, 57, pp.301~323 (1999)
- 김관중, "Neuroprotective effects of ethanolic extract from dry Rhodiola rosea L. rhizomes", Food Science and Biotechnology, 30(2), pp.287~297 (2021), 한국식품과학회[DOI]
- Mattson MP, "Neuroprotective and neurorestorative signal transduction mechanisms in brain aging: modification by genes, diet and behavior", Neurobiol. Aging, 23, pp.695~705 (2002)
- Suematsu N, "Protective effects of quercetin against hydrogen peroxide-induced apoptosis in human neuronal SH-SY5Y cells", Neurosci. Lett, 504, pp.223~227 (2011)
- Lu B, "Handb. Exp. Pharmacol. 220", pp.223~250 (2014)
- Daubner SC, "Tyrosine hydroxylase and regulation of dopamine synthesis", Arch. Biochem. Biophys, 508, pp.1~12 (2011)
- Cryan JF, "The microbiota-gut-brain axis", Physiol. Rev, 99, pp.1877~2013 (2019)
- Westfall S, "Microbiome, probiotics and neurodegenerative diseases:deciphering the gut brain axis", Cell. Mol. Life. Sci, 74, pp.3769~3787 (2017)
- 김기태, "Probiotic properties of novel probiotic Levilactobacillus brevis KU15147 isolated from radish kimchi and its antioxidant and immune-enhancing activities", Food Science and Biotechnology, 30(2), pp.257~265 (2021), 한국식품과학회[DOI]
- Gazerani P, "Probiotics for Parkinson’s disease", Int. J. Mol. Sci, 20, pp.4121~ (2019)
- 이예원, "Probiotics-Mediated Bioconversion and Periodontitis", 한국축산식품학회지, 41(6), pp.905~922 (2021), 한국축산식품학회[DOI]
- Parashar A, "Gut microbiota: Implications in Parkinson's disease", Parkinsonism Relat. Disord, 38, pp.1~7 (2017)
- Basavaprabhu H. Nataraj, "Evolutionary concepts in the functional biotics arena: a mini-review", Food Science and Biotechnology, 30(4), pp.487~496 (2021), 한국식품과학회[DOI]
- Srivastav S, "Probiotics mixture increases butyrate, and subsequently rescues the nigral dopaminergic neurons from MPTP and rotenone-induced neurotoxicity", J. Nutr. Biochem, 69, pp.73~86 (2019)
- Tan AH, "Probiotics for Parkinson's disease: current evidence and future directions", JGH Open, 5, pp.414~419 (2021)
- Teame T, "Paraprobiotics and postbiotics of probiotic lactobacilli, their positive effects on the host and action mechanisms: A review", Front. Nutr, 7, pp.570344~ (2020)
- Wagner RD, "Probiotic effects of feeding heat-killed Lactobacillus acidophilus and Lactobacillus casei to Candida albicans-colonized immunodeficient mice", J. Food Prot, 63, pp.638~644 (2000)
- Piqué N, "Health benefits of heat-killed (tyndallized) probiotics: An overview", Int. J. Mol. Sci, 20, pp.2534~ (2019)
- Kim Won-Ju, "Protective Effects of a Novel Lactobacillus brevis Strain with Probiotic Characteristics against Staphylococcus aureus Lipoteichoic Acid-Induced Intestinal Inflammatory Response", Journal of Microbiology and Biotechnology, 32(2), pp.205~211 (2022), 한국미생물·생명공학회[DOI]
- Bagci EZ, "Bistability in apoptosis: roles of bax, bcl-2, and mitochondrial permeability transition pores", Biophys. J, 90, pp.1546~1559 (2006)
- Wang ZH, "Therapeutic effects of astrocytes expressing both tyrosine hydroxylase and brain-derived neurotrophic factor on a rat model of Parkinson’s disease", Neurosci, 113, pp.629~640 (2002)
- Song MW, "Probiotic characteristics of Lactobacillus brevis B13-2 isolated from kimchi and investigation of antioxidant and immune-modulating abilities of its heat-killed cells", LWT-Food Sci. Technol, 128, pp.109452~ (2020)
- 장혜지, "Physicochemical analysis of non-fermented probiotic milk with probiotic Lactobacillus plantarum Ln1 isolated from Korea traditional fermented food", Food Science and Biotechnology, 31(6), pp.731~737 (2022), 한국식품과학회[DOI]
- Cheon MJ, "Probiotic properties and neuroprotective effects of Lactobacillus buchneri KU200793isolated from Korean fermented foods", Int. J. Mol. Sci, 21, pp.1227~ (2020)
- Choi GH, "Soy yogurt using Lactobacillus plantarum 200655 and fructooligosaccharides:neuroprotective effects against oxidative stress", J. Food Sci. Technol, 59, pp.4870~4879 (2022)
- Nirmaladevi D, "Neuroprotective effects of bikaverin on H2O2-induced oxidative stress mediated neuronal damage in SH-SY5Y cell line", Cell. Mol. Neurobiol, 34, pp.973~985 (2014)
- 송명욱, "Probiotic and Antioxidant Properties of Novel Lactobacillus brevis KCCM 12203P Isolated from Kimchi and Evaluation of Immune- Stimulating Activities of Its Heat-Killed Cells in RAW 264.7 Cells", Journal of Microbiology and Biotechnology, 29(12), pp.1894~1903 (2019), 한국미생물·생명공학회[DOI]
- 이나경, "Physicochemical Analysis of Yogurt Produced by Leuconostoc mesenteroides H40 and Its Effects on Oxidative Stress in Neuronal Cells", 한국축산식품학회지, 41(2), pp.261~273 (2021), 한국축산식품학회[DOI]
- Enari M, "A caspase-activated DNase that degrades DNA during apoptosis, and its inhibitor ICAD", Nature, 391, pp.43~50 (1998)
- Park J, "Neuroprotective effect of Ruminococcus albus on oxidatively stressed SH-SY5Y cells and animals", Sci. Rep, 7, pp.14520~ (2017)
- Jang HM, "The preventive and curative effects of Lactobacillus reuteri NK33 and Bifidobacterium adolescentis NK98 on immobilization stress-induced anxiety", depression and colitis in mice, 2019, pp.11, 819~
- Liao JF, "Lactobacillus plantarum PS128 alleviates neurodegenerative progression in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced mouse models of Parkinson’s disease", Brain. Behav. Immun, 90, pp.26~46 (2020)
- Wang L, "Neuroprotective effect of Lactobacillus plantarum DP189 on MPTPinduced Parkinson's disease model mice", J. Funct. Foods, 85, pp.104635~ (2021)
- Behl C, "Antioxidant neuroprotection in Alzheimer’s disease as preventive and therapeutic approach", Free Radic. Biol. Med, 33, pp.182~191 (2002)
- Floegel A, "Comparison of ABTS", DPPH assays to measure antioxidant capacity in popular antioxidant-rich US foods, 2011, pp.24, 1043~1048
- Nie P, "Protective effect of Lactobacillus rhamnosus GG on TiO2 nanoparticles-induced oxidative stress damage in the liver of young rats", Nanomaterials, 11, pp.803~ (2021)
- Choi BS, "Antioxidant activity and protective effects of Tripterygium regelii extract on hydrogen peroxide-induced injury in human dopaminergic cells, SH-SY5Y", Neurochem. Res, 35, pp.1269~1280 (2010)
- Choi DJ, "Neuroprotective effects of corn silk maysin via inhibition of H2O2-induced apoptotic cell death in SK-N-MC cells", Life Sci, 109, pp.57~64 (2014)
- Sun FL, "Tetrahydroxystilbene glucoside protects human neuroblastoma SH-SY5Y cells against MPP+-induced cytotoxicity", Eur. J. Pharmacol, 660, pp.283~290 (2011)
- Mehri S, "Neuroprotective effect of crocin on acrylamide-induced cytotoxicity in PC12 cells", Cell. Mol. Neurobiol, 32, pp.227~235 (2012)
- Zhao DL, "6,7-Di-O-glucopyranosyl-esculetin protects SH-SY5Y cells from dopamine-induced cytotoxicity", Eur. J. Pharmacol, 580, pp.329~338 (2008)
- Bavari M, "Neuroprotective, antiapoptotic and antioxidant effects of Lcarnitine against caffeine-induced neurotoxicity in SH-SY5Y neuroblastoma cell line", Drug Chem. Toxicol, 39, pp.157~166 (2016)
- Ju HY, "Antioxidant phenolic profile from ethyl acetate fraction of Fructus Ligustri Lucidi with protection against hydrogen peroxide-induced oxidative damage in SH-SY5Y cells", Food Chem. Toxicol, 50, pp.492~502 (2012)
- Kkot Byeol Kim, "Neuroprotective effects of urolithin A on H2O2-induced oxidative stress-mediated apoptosis in SK-N-MC cells", Nutrition Research and Practice, 14(1), pp.3~11 (2020), 한국영양학회[DOI]
