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Mesorhizobium koreense sp. nov., Isolated from Soil

김동욱|Chaudhary Dhiraj Kumar|Lee Hyosun
한국미생물·생명공학회 (The Korean Society For Microbiology And Biotechnology)|2024.09|Journal of Microbiology and Biotechnology|제34권 제9호|pp. 1819-1825|DOI 10.4014/jmb.2404.04026

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참고문헌

키워드

phylogeneticsPseudomonadotaMesorhizobium koreense sp. nov. soilPhyllobacteriaceae
주제분류생물학

초록

An aerobic, Gram-stain-negative, catalase-positive, rod-shaped, and motile bacteria, designated as a strain WR6T was isolated from soil in Republic of Korea. Strain WR6T grew at temperatures of 10– 37°C, at pH of 5.0–9.0, and at NaCl concentrations of 0–3.0% (w/v). Phylogenetic and 16S rRNA gene nucleotide sequence analysis confirmed that strain WR6T affiliated to the genus Mesorhizobium, with the nearest relative being Mesorhizobium waimense ICMP 19557T (98.5%). The genome of strain WR6T was 5,035,462 bp with DNA G+C content of 62.6%. In strain WR6T , Q-10 was sole ubiquinone; summed feature 8 (C18:1ω7c and/or C18: 1ω6c) and C19:0 cyclo ω8c were predominant fatty acids; and diphosphatidylglycerol, phosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylcholine, and phosphatidylethanolamine were major polar lipids. Based on these polyphasic taxonomic data, strain WR6T represents a novel species in the genus Mesorhizobium. Accordingly, we propose the name Mesorhizobium koreense sp. nov., with the type strain WR6T (=KCTC 92695T =NBRC 116021T ).

Abstract

An aerobic, Gram-stain-negative, catalase-positive, rod-shaped, and motile bacteria, designated as a strain WR6T was isolated from soil in Republic of Korea. Strain WR6T grew at temperatures of 10– 37°C, at pH of 5.0–9.0, and at NaCl concentrations of 0–3.0% (w/v). Phylogenetic and 16S rRNA gene nucleotide sequence analysis confirmed that strain WR6T affiliated to the genus Mesorhizobium, with the nearest relative being Mesorhizobium waimense ICMP 19557T (98.5%). The genome of strain WR6T was 5,035,462 bp with DNA G+C content of 62.6%. In strain WR6T , Q-10 was sole ubiquinone; summed feature 8 (C18:1ω7c and/or C18: 1ω6c) and C19:0 cyclo ω8c were predominant fatty acids; and diphosphatidylglycerol, phosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylcholine, and phosphatidylethanolamine were major polar lipids. Based on these polyphasic taxonomic data, strain WR6T represents a novel species in the genus Mesorhizobium. Accordingly, we propose the name Mesorhizobium koreense sp. nov., with the type strain WR6T (=KCTC 92695T =NBRC 116021T ).

참고문헌 (42)

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