본문 바로가기
KERD
논문KCI 등재

Anti‐Inflammatory Response in TNFα/IFNγ- Induced HaCaT Keratinocytes and Probiotic Properties of Lacticaseibacillus rhamnosus MG4644, Lacticaseibacillus paracasei MG4693, and Lactococcus lactis MG5474

0

조회

0

다운로드

1

인용

0

참고문헌

키워드

주제분류생물학

초록

Atopic dermatitis (AD) is a chronic inflammatory disease caused by immune dysregulation. Meanwhile, the supernatant of lactic acid bacteria (SL) was recently reported to have anti-inflammatory effects. In addition, HaCaT keratinocytes stimulated by tumor necrosis factor alpha (TNF-α) and interferon gamma (IFN-γ) are widely used for studying AD‐like responses. In this study, we evaluated the antiinflammatory effects of SL from lactic acid bacteria (LAB) on TNF-α/IFN-γ-induced HaCaT keratinocytes, and then we investigated the strains’ probiotic properties. SL was noncytotoxic and regulated chemokines (macrophage-derived chemokine (MDC) and thymus and activation-regulated chemokine (TARC)) and cytokines (interleukin (IL)‐4, IL‐5, IL‐25, and IL‐33) in TNF-α/IFN-γ-induced HaCaT keratinocytes. SL from Lacticaseibacillus rhamnosus MG4644, Lacticaseibacillus paracasei MG4693, and Lactococcus lactis MG5474 decreased the phosphorylation of nuclear factor-κB (NF-κB) and mitogen-activated protein kinase (MAPK). Furthermore, the safety of the three strains was demonstrated via hemolysis, bile salt hydrolase (BSH) activity, and toxicity tests, and the stability was confirmed under simulated gastrointestinal conditions. Therefore, L. rhamnosus MG4644, L. paracasei MG4693, and Lc. lactis MG5474 have potential applications in functional food as they are stable and safe for intestinal epithelial cells and could improve atopic inflammation.

Abstract

Atopic dermatitis (AD) is a chronic inflammatory disease caused by immune dysregulation. Meanwhile, the supernatant of lactic acid bacteria (SL) was recently reported to have anti-inflammatory effects. In addition, HaCaT keratinocytes stimulated by tumor necrosis factor alpha (TNF-α) and interferon gamma (IFN-γ) are widely used for studying AD‐like responses. In this study, we evaluated the antiinflammatory effects of SL from lactic acid bacteria (LAB) on TNF-α/IFN-γ-induced HaCaT keratinocytes, and then we investigated the strains’ probiotic properties. SL was noncytotoxic and regulated chemokines (macrophage-derived chemokine (MDC) and thymus and activation-regulated chemokine (TARC)) and cytokines (interleukin (IL)‐4, IL‐5, IL‐25, and IL‐33) in TNF-α/IFN-γ-induced HaCaT keratinocytes. SL from Lacticaseibacillus rhamnosus MG4644, Lacticaseibacillus paracasei MG4693, and Lactococcus lactis MG5474 decreased the phosphorylation of nuclear factor-κB (NF-κB) and mitogen-activated protein kinase (MAPK). Furthermore, the safety of the three strains was demonstrated via hemolysis, bile salt hydrolase (BSH) activity, and toxicity tests, and the stability was confirmed under simulated gastrointestinal conditions. Therefore, L. rhamnosus MG4644, L. paracasei MG4693, and Lc. lactis MG5474 have potential applications in functional food as they are stable and safe for intestinal epithelial cells and could improve atopic inflammation.

참고문헌 (48)

  1. Torres T, "Update on atopic dermatitis", Acta Medica Port, 32, pp.606~613 (2019)
  2. 유주환, "Impairment of Quality of Life and Mental Health Status in Adult-Onset Atopic Dermatitis", Annals of Dermatology, 34(4), pp.278~286 (2022), 대한피부과학회[DOI]
  3. Ha Jihyun, "Ten-Year trends and prevalence of asthma, allergic rhinitis, and atopic dermatitis among the Korean population, 2008–2017", Clinical and Experimental Pediatrics, 63(7), pp.278~283 (2020), 대한소아청소년과학회[DOI]
  4. Manjelievskaia J, "The direct and indirect costs of adult atopic dermatitis", J. Manag. Care Spec. Pharm, 27, pp.1416~1425 (2021)
  5. Albader SS, "Dupilumab side effect in a patient with atopic dermatitis: a case report study", Biologics, 13, pp.79~82 (2019)
  6. Lee JY, "Improvements in human keratinocytes and antimicrobial effect mediated by cell-free supernatants derived from probiotics", Fermentation, 8, pp.332~ (2022)
  7. Bitschar K, "Keratinocytes as sensors and central players in the immune defense against Staphylococcus aureus in the skin", J. Dermatol. Sci, 87, pp.215~220 (2017)
  8. Albanesi C, "Keratinocytes in inflammatory skin diseases", Curr. Drug Targets Inflamm. Allergy, 4, pp.329~334 (2005)
  9. Cho SH, "Eckol from Ecklonia cava ameliorates TNF-α", IFN-γ-induced inflammatory responses via regulating MAPKs and NF-κB signaling pathway in HaCaT cells, 2020, pp.82, 106146~
  10. Chieosilapatham P, "Keratinocytes: Innate immune cells in atopic dermatitis", Clin. Expe. Immunol, 204, pp.296~309 (2021)
  11. Yan F, "The formulae and biologically active ingredients of Chinese herbal medicines for the treatment of atopic dermatitis", Biomed. Pharmacother, 127, pp.110142~ (2020)
  12. Edalati E, "Isolation of probiotic bacteria from raw camel's milk and their antagonistic effects on two bacteria causing food poisoning", New Microb. New Infect, 27, pp.64~68 (2019)
  13. Hill C, "The international scientific association for robiotics and prebiotics consensus statement on the scope and appropriate use of the term probiotic", Nat. Rev. Gastroenterol. Hepatol, 11, pp.506~514 (2014)
  14. Lizardo MV, "Probiotics", pp.389~405 (2022), Elsevier
  15. Rusu E, "Prebiotics and probiotics in atopic dermatitis", Exp. Ther. Med, 18, pp.926~931 (2019)
  16. Lew LC, "Bioactives from probiotics for dermal health: functions and benefits", J. Appl. Microbiol, 114, pp.1241~1253 (2013)
  17. Lee JY, "Lactobacillus gasseri MG4247 and Lacticaseibacillus paracasei MG4272 and MG4577modulate allergic inflammatory response in RAW 264.7 and RBL-2H3 cells", Probiotics Antimicrob. Proteins, 31, pp.1~10 (2022)
  18. Tolosa L, "Protocols in in vitro hepatocyte research", pp.333~348 (2015), Springer
  19. Livak KJ, "Analysis of relative gene expression data using real-time quantitative PCR and the 2− ΔΔCT method", Methods, 25, pp.402~408 (2001)
  20. Bass JJ, "An overview of technical considerations for Western blotting applications to physiological research", Scand. J. Med. Sci. Sports, 27, pp.4~25 (2017)
  21. Park JY, "Latilactobacillus sakei Wikim0066 protects skin through MMP regulation on UVB-irradiated in vitro and in vivo model", Nutrients, 15, pp.726~ (2023)
  22. Syed Yaacob SN, "Identification of Lactobacillus spp. and Fructobacillus spp. isolated from fresh Heterotrigona itama honey and their antagonistic activities against clinical pathogenic bacteria", J. Apic. Res, 57, pp.395~405 (2018)
  23. 강창호, "유아 분변에서 분리한 비피도박테리아의 프로바이오틱스 기능성 연구", Journal of Dairy Science and Biotechnology, 37(1), pp.40~48 (2019), 한국낙농식품응용생물학회[DOI]
  24. Yasmin I, "In vitro probiotic potential and safety evaluation (hemolytic, cytotoxic activity) of Bifidobacterium strains isolated from raw camel milk", Microorganisms, 8, pp.354~ (2020)
  25. Buxton R, "Blood agar plates and hemolysis protocols", American Society for Microbiology, 1~9 (2005)
  26. Shehata M, "Screening of isolated potential probiotic lactic acid bacteria for cholesterol lowering property and bile salt hydrolase activity", Ann. Agric. Sci, 61, pp.65~75 (2016)
  27. Kim S, "Antioxidant activity and probiotic properties of lactic acid bacteria", Fermentation, 8, pp.29~ (2022)
  28. Fonseca HC, "Probiotic properties of lactobacilli and their ability to inhibit the adhesion of enteropathogenic bacteria to Caco-2 and HT-29 cells", Probiotics Antimicrob. Proteins, 13, pp.102~112 (2021)
  29. Cheng KW, "Inhibition of gut microbial β-glucuronidase effectively prevents carcinogen-induced microbial dysbiosis and intestinal tumorigenesis", Pharmacol. Res, 177, pp.106115~ (2022)
  30. De Pessemier B, "Gut–skin axis: current knowledge of the interrelationship between microbial dysbiosis and skin conditions", Microorganisms, 9, pp.353~ (2021)
  31. 이소연, "Microbiome in the Gut-Skin Axis in Atopic Dermatitis", Allergy, Asthma & Immunology Research, 10(4), pp.354~362 (2018), 대한천식알레르기학회
  32. Lolou V, "Functional role of probiotics and prebiotics on skin health and disease", Fermentation, 5, pp.41~ (2019)
  33. De Vuyst E, "Atopic dermatitis studies through in vitro models", Front. Med, 4, pp.119~ (2017)
  34. Song HK, "Spatholobus suberectus dunn water extract ameliorates atopic dermatitis–like symptoms by suppressing proinflammatory chemokine production in vivo and in vitro", Front. Pharmacol, 13, pp.919230~ (2022)
  35. Hwang DH, "Rosa davurica Pall. improves DNCB-induced atopic dermatitis in mice and regulated TNF-alpha", IFN-gamma-induced skin inflammatory responses in HaCaT cells, 2021, pp.91, 153708~
  36. Yang CC, "Effect of neferine on DNCB-induced atopic dermatitis in HaCaT cells and BALB", c mice, 2021, pp.22, 8237~
  37. Sebastian K, "Signal transduction around thymic stromal lymphopoietin (TSLP) in atopic asthma", Cell Commun. Signal, 6, pp.5~ (2008)
  38. Divekar R, "Recent advances in epithelium-derived cytokines (IL-33, IL-25 and TSLP) and allergic inflammation", Curr. Opin. Allergy Clin. Immunol, 15, pp.98~ (2015)
  39. Xiao X, "The role of short-chain fatty acids in inflammatory skin diseases", Front. Microbiol, 13, pp.1083432~ (2022)
  40. Kang CH, "Antioxidant activity and short-chain fatty acid production of lactic acid bacteria isolated from Korean individuals and fermented foods", 3 Biotech, 11, pp.217~ (2021)
  41. Aryantini NPD, "In vitro safety assessments and antimicrobial activities of Lactobacillus rhamnosus strains isolated from a fermented mare's milk", Anim. Sci. J, 88, pp.517~525 (2017)
  42. Meerloo JV, "Cancer cell culture", pp.237~245 (2011), Springer
  43. Ranjan KG, "In vitro evaluation of antibacterial, cytotoxic and adherence studies of selected commercial probiotics", J. Pure Appl. Microbiol, 14, pp.2085~2091 (2020)
  44. Khare A, "Cholesterol-lowering effects of Lactobacillus species", Curr. Microbiol, 77, pp.638~644 (2020)
  45. Byakika S, "A review of criteria and methods for evaluating the probiotic potential of microorganisms", Food Rev. Int, 35, pp.427~466 (2019)
  46. Casarotti SN, "Effect of different matrices on probiotic resistance to in vitro simulated gastrointestinal conditions", Int. J. Dairy Technol, 68, pp.595~601 (2015)
  47. Jelena Novaković Jovanović, "Characterization of Some Potentially Probiotic Lactobacillus Strains of Human Origin", Food Science and Biotechnology, 24(5), pp.1781~1788 (2015), 한국식품과학회[DOI]
  48. Verhoeckx K, "The impact of food bioactives on health: in vitro and ex vivo models" (2015), Springer

관련 논문AI추천

Potential Anti-Allergy and Immunomodulatory Properties of Lactococcus lactis LB 1022 Observed In Vitro and in an Atopic Dermatitis Mouse Model김원용, Baek Jihye, Kim Jong-Hwa한국미생물·생명공학회 · Journal of Microbiology and Biotechnology2023 · 제33권 제6호Anti-inflammatory effects of Lactobacillus reuteri LM1071 via MAP kinase pathway in IL-1β-induced HT-29 cells김태락, 지요셉, Kyoung-sook Choi, Min-Gyu Jeon, Wilhelm H. Holzapfel한국축산학회 · 한국축산학회지2020 · 제62권 제6호Safety, Antagonistic Activity, and Probiotic Properties of Lactic Acid Bacteria Isolated from Jeotgal, Korean Fermented SeafoodsBaek Jihyeon, Bai Jaewoo, Kim Bong Sun, Kim Yeonju한국미생물·생명공학회 · Journal of Microbiology and Biotechnology2025 · 제35권 Enhancement of Skin Antioxidant and Anti-Inflammatory Potentials of Agastache rugosa Leaf Extract by Probiotic Bacterial Fermentation in Human Epidermal Keratinocytes김경훈, 윤지영, 이수희, 이윤진, 임창진, 임혜원, 황옥화한국미생물·생명공학회 · 한국미생물·생명공학회지2017 · 제45권 제1호Strain-Level Differences of Bifidobacterium breve in the Gut Microbiome between Infants with and without Atopic Dermatitis: Insights from Genome Analysis and Immune AssaysKim Bong-Soo, Kim Seong Hee, Lee Imchang, Lee Kwang Jun, Lee Min-Jung, Lee Yun Kyung, Oh Ara한국미생물·생명공학회 · Journal of Microbiology and Biotechnology2025 · 제35권 Heat-Treated Paraprobiotic Latilactobacillus sakei KU15041 and Latilactobacillus curvatus KU15003 Show an Antioxidant and Immunostimulatory Effect강대경, 박영서, 백현동, 이나경, Hyun Jun-Hyun, Kim Kee-Tae, Woo Im-Kyung한국미생물·생명공학회 · Journal of Microbiology and Biotechnology2024 · 제34권 제2호

이 간행물의 다른 논문 (14건)