Amygdalin Reverses Macrophage PANoptosis Induced by Drug-Resistant Escherichia coli
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Infectious diseases caused by drug-resistant Escherichia coli (E. coli) pose a critical concern for medical institutions as they can lead to high morbidity and mortality rates. In this study, amygdalin exhibited anti-inflammatory and antioxidant activities, as well as other potentials. However, whether it could influence the drug-resistant E. coli-infected cells remained unanswered. Amygdalin was therefore tested in a cellular model in which human macrophages were exposed to resistant E. coli. Apoptosis was measured by flow cytometry and the lactate dehydrogenase (LDH) assay. Western immunoblotting and quantitative reverse-transcription polymerase chain reaction (qRT-PCR) were used to quantify interleukin-18 (IL-18), interleukin-1β (IL-1β), and interleukin-6 (IL-6). The production of reactive oxygen species (ROS) in macrophages was detected by ROS kit. The expression of panapoptotic proteins in macrophages was measured by qRT-PCR and Western immunoblotting. Drug-Resistant E. coli inhibited cell viability and enhanced apoptosis in the cellular model. In cells treated with amygdalin, this compound can inhibit cell apoptosis and reduce the expression of pro - inflammatory cytokines such as IL-1β, IL-18 and IL-6. Additionally, it decreases the production of PANoptosis proteins, Furthermore, amygdalin lowered the levels of reactive oxygen species induced by drug-resistant E. coli, in cells, demonstrating its antioxidant effects. Amygdalin, a drug with a protective role, alleviated cell damage caused by drug-resistant E. coli in human macrophages by inhibiting the PANoptosis signaling pathway.
Abstract
Infectious diseases caused by drug-resistant Escherichia coli (E. coli) pose a critical concern for medical institutions as they can lead to high morbidity and mortality rates. In this study, amygdalin exhibited anti-inflammatory and antioxidant activities, as well as other potentials. However, whether it could influence the drug-resistant E. coli-infected cells remained unanswered. Amygdalin was therefore tested in a cellular model in which human macrophages were exposed to resistant E. coli. Apoptosis was measured by flow cytometry and the lactate dehydrogenase (LDH) assay. Western immunoblotting and quantitative reverse-transcription polymerase chain reaction (qRT-PCR) were used to quantify interleukin-18 (IL-18), interleukin-1β (IL-1β), and interleukin-6 (IL-6). The production of reactive oxygen species (ROS) in macrophages was detected by ROS kit. The expression of panapoptotic proteins in macrophages was measured by qRT-PCR and Western immunoblotting. Drug-Resistant E. coli inhibited cell viability and enhanced apoptosis in the cellular model. In cells treated with amygdalin, this compound can inhibit cell apoptosis and reduce the expression of pro - inflammatory cytokines such as IL-1β, IL-18 and IL-6. Additionally, it decreases the production of PANoptosis proteins, Furthermore, amygdalin lowered the levels of reactive oxygen species induced by drug-resistant E. coli, in cells, demonstrating its antioxidant effects. Amygdalin, a drug with a protective role, alleviated cell damage caused by drug-resistant E. coli in human macrophages by inhibiting the PANoptosis signaling pathway.
참고문헌 (45)
- Riley LW, "Distinguishing pathovars from nonpathovars: Escherichia coli", Microbiol. Spectr, 8 (2020)[DOI]
- Dolatyar Dehkharghani A, "Clonal relationship and resistance profiles among ESBL-producing Escherichia coli", Front. Cell. Infect. Microbiol, 11, pp.560622~ (2021)
- Paitan Y, "Current trends in antimicrobial resistance of Escherichia coli", Curr. Topics Microbiol. Immunol, 416, pp.181~211 (2018)
- Peng XQ, "Antiviral effects of Yinhuapinggan granule against influenza virus infection in the ICR mice model", J. Nat. Med, 70, pp.75~88 (2016)
- Jin L, "Investigation of pharmacological mechanisms of Yinhua Pinggan Granule on the treatment of Pneumonia through network pharmacology and in vitro", Biomed Res. Int, 2022, pp.1602447~ (2022)
- Guo P, "Glycyrrhetinic acid protects against multidrug-resistant Acinetobacter baumannii-induced lung epithelial cells injury by regulating inflammation and oxidative stress", BMC Pharmacol. Toxicol, 24, pp.5~ (2023)
- Jiagang D, "Amygdalin mediates relieved atherosclerosis in apolipoprotein E deficient mice through the induction of regulatory T cells", Biochem. Biophys. Res. Commun, 411, pp.523~529 (2011)
- Makarević J, "Amygdalin blocks bladder cancer cell growth in vitro by diminishing cyclin A and cdk2", PLoS One, 9, pp.e105590~ (2014)
- He XY, "Amygdalin - A pharmacological and toxicological review", J. Ethnopharmacol, 254, pp.112717~ (2020)
- Costantini PE, "Insight into phenotypic and genotypic differences between vaginal Lactobacillus crispatus BC5 and Lactobacillus gasseri BC12 to unravel nutritional and stress factors influencing their metabolic activity", Microb. Genom, 7, pp.000575~ (2021)
- 황혜정, "Inhibitory Effect of Amygdalin on Lipopolysaccharide-Inducible TNF-α and IL-1β mRNA Expression and Carrageenan-Induced Rat Arthritis", Journal of Microbiology and Biotechnology, 18(10), pp.1641~1647 (2008), 한국미생물·생명공학회
- Malireddi RKS, "ZBP1 and TAK1: master regulators of NLRP3 inflammasome", pyroptosis, apoptosis, and necroptosis (PAN-optosis), 2019, pp.9, 406~
- Lee S, "AIM2 forms a complex with pyrin and ZBP1 to drive PANoptosis and host defence", Nature, 597, pp.415~419 (2021)
- Zheng M, "The regulation of the ZBP1-NLRP3 inflammasome and its implications in pyroptosis, apoptosis, and necroptosis (PANoptosis)", Immunol. Rev, 297, pp.26~38 (2020)
- Gurung P, "NLRP3 inflammasome plays a redundant role with caspase 8 to promote IL-1β-mediated osteomyelitis", Proc. Natl. Acad. Sci. USA, 113, pp.4452~4457 (2016)
- Lukens JR, "Dietary modulation of the microbiome affects autoinflammatory disease", Nature, 516, pp.246~249 (2014)
- Samir P, "The PANoptosome: a deadly protein complex driving pyroptosis, apoptosis, and necroptosis (PANoptosis)", Front. Cell. Infect. Microbiol, 10, pp.238~ (2020)
- Cui Y, "MiR-29a-3p Improves acute lung injury by reducing alveolar epithelial cell PANoptosis", Aging Dis, 13, pp.899~909 (2022)
- Christgen S, "Identification of the PANoptosome: a molecular platform triggering Pyroptosis, Apoptosis, and Necroptosis (PANoptosis)", Front. Cell. Infect. Microbiol, 10, pp.237~ (2020)
- Dunn SJ, "The evolution and transmission of multi-drug resistant Escherichia coli and Klebsiella pneumoniae: the complexity of clones and plasmids", Curr. Opin. Microbiol, 51, pp.51~56 (2019)
- Wei R, "Tagitinin C induces ferroptosis through PERK-Nrf2-HO-1 signaling pathway in colorectal cancer cells", Int. J. Biol. Sci, 17, pp.2703~2717 (2021)
- Kaper JB, "Pathogenic Escherichia coli", Nat. Rev. Microbiol, 2, pp.123~140 (2004)
- Harris PN, "β-lactam and β-lactamase inhibitor combinations in the treatment of extendedspectrum β-lactamase producing Enterobacteriaceae: time for a reappraisal in the era of few antibiotic options?", Lancet Infect. Dis, 15, pp.475~485 (2015)
- Stocks CJ, "Uropathogenic Escherichia coli employs both evasion and resistance to subvert innate immune-mediated zinc toxicity for dissemination", Proc. Natl. Acad. Sci. USA, 116, pp.6341~6350 (2019)
- Ulett GC, "Uropathogenic Escherichia coli virulence and innate immune responses during urinary tract infection", Curr. Opin. Microbiol, 16, pp.100~107 (2013)
- Guan X, "Glycyrrhetinic acid prevents carbapenem-resistant Klebsiella pneumoniaeinduced cell injury by inhibiting mitochondrial dysfunction via Nrf-2 pathway", Microb. Pathog, 177, pp.105825~ (2022)
- Si Z, "Amygdalin attenuates airway epithelium apoptosis, inflammation, and epithelial-mesenchymal transition through restraining the TLR4", NF-κB signaling pathway on LPS-treated BEAS-2B bronchial epithelial cells, 2021, pp.182, 997~1007
- Gluschko A, "The β(2) integrin Mac-1 induces protective LC3-associated phagocytosis of Listeria monocytogenes", Cell Host Microbe, 23, pp.324~337.e325 (2018)
- Pollock JD, "Mouse model of X-linked chronic granulomatous disease, an inherited defect in phagocyte superoxide production", Nat. Genet, 9, pp.202~209 (1995)
- West AP, "TLR signalling augments macrophage bactericidal activity through mitochondrial ROS", Nature, 472, pp.476~480 (2011)
- Winterbourn CC, "Redox reactions and microbial killing in the neutrophil phagosome", Antioxid. Redox Signal, 18, pp.642~660 (2013)
- Sies H, "Reactive Oxygen Species (ROS) as pleiotropic physiological signalling agents", Nat. Rev. Mol. Cell Biol, 21, pp.363~383 (2020)
- Niki E, "Oxidative stress and antioxidants: distress or eustress?", Arch. Biochem. Biophys, 595, pp.19~24 (2016)
- Herb M, "Mitochondrial reactive oxygen species enable proinflammatory signaling through disulfide linkage of NEMO", Sci. Signal, 12, pp.eaar5926~ (2019)
- Craig M, "Phagocytic superoxide specifically damages an extracytoplasmic target to inhibit or kill Salmonella", PLoS One, 4, pp.e4975~ (2009)
- Wink DA, "Nitric oxide and redox mechanisms in the immune response", J. Leukoc. Biol, 89, pp.873~891 (2011)
- Slauch JM, "How does the oxidative burst of macrophages kill bacteria? Still an open question", Mol. Microbiol, 80, pp.580~583 (2011)
- Hayes JD, "Oxidative stress in cancer", Cancer Cell, 38, pp.167~197 (2020)
- Ju HQ, "Modulation of redox homeostasis by inhibition of MTHFD2 in colorectal cancer: mechanisms and therapeutic implications", J. Nat. Cancer Inst, 111, pp.584~596 (2019)
- Zhou B, "Tom20 senses iron-activated ROS signaling to promote melanoma cell pyroptosis", Cell Res, 28, pp.1171~1185 (2018)
- Chen J, "Amygdalin alleviates renal injury by suppressing inflammation, oxidative stress and fibrosis in streptozotocin-induced diabetic rats", Life Sci, 265, pp.118835~ (2021)
- Tian Y, "Photothermal therapy with regulated Nrf2", NF-κB signaling pathway for treating bacteria-induced periodontitis, 2022, pp.9, 428~445
- Karki R, "ADAR1 restricts ZBP1-mediated immune response and PANoptosis to promote tumorigenesis", Cell Rep, 37, pp.109858~ (2021)
- Lin JF, "Phosphorylated NFS1 weakens oxaliplatin-based chemosensitivity of colorectal cancer by preventing PANoptosis", Signal Transduct. Target. Ther, 7, pp.54~ (2022)
- Müller S, "IGF2BP1 promotes SRF-dependent transcription in cancer in a m6A- and miRNA-dependent manner", Nucleic Acids Res, 47, pp.375~390 (2019)