Flavonoids Fraction of Mespilus Germanica Alleviates Insulin Resistance in Metabolic Syndrome Model of Ovariectomized Rats via Reduction in Tumor Necrosis Factor-α
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Objectives: The rate of metabolic syndrome (MetS) in women diagnosed as they age is one of the main concerns of health cares. Recently new strategies used to prevent progressions of MetS toward the diagnosis of diabetes have focused on plant flavonoids. This study was aimed to investigate the beneficial effects of flavonoids fraction of Mespilus germanica leaves (MGL) on MetS in ovariectomized (OVX) rats.Methods: Twenty-four adult female Wistar rats, weighing 200 to 250 g, were divided into 3 groups: Sham surgery, OVX + Salin, or OVX + Flavonoid. Three weeks after ovariectomy, animals displayed MetS criteria received flavonoid injection (10 mg/kg, intraperitoneally) for 21 days. Then the body weight, body mass index, waist circumference, visceral fat, fasting blood glucose, serum insulin, lipid profiles and tumor necrosis factor-α (TNF-α) were measured. Results: Treatment with flavonoids fraction of MGL significantly decreased serum level of insulin (P = 0.011), glucose (P = 0.024), TNF-α (P = 0.010), also MetS Z score (P = 0.020) and homeostasis model assessment of insulin resistance (P = 0.007). Lipid profiles and visceral fat showed insignificant reduction. Conclusions: Flavonoids of MGL attenuates some of the MetS components possibly via reduction in TNF-α inflammatory cytokine.
Abstract
Objectives: The rate of metabolic syndrome (MetS) in women diagnosed as they age is one of the main concerns of health cares. Recently new strategies used to prevent progressions of MetS toward the diagnosis of diabetes have focused on plant flavonoids. This study was aimed to investigate the beneficial effects of flavonoids fraction of Mespilus germanica leaves (MGL) on MetS in ovariectomized (OVX) rats.Methods: Twenty-four adult female Wistar rats, weighing 200 to 250 g, were divided into 3 groups: Sham surgery, OVX + Salin, or OVX + Flavonoid. Three weeks after ovariectomy, animals displayed MetS criteria received flavonoid injection (10 mg/kg, intraperitoneally) for 21 days. Then the body weight, body mass index, waist circumference, visceral fat, fasting blood glucose, serum insulin, lipid profiles and tumor necrosis factor-α (TNF-α) were measured. Results: Treatment with flavonoids fraction of MGL significantly decreased serum level of insulin (P = 0.011), glucose (P = 0.024), TNF-α (P = 0.010), also MetS Z score (P = 0.020) and homeostasis model assessment of insulin resistance (P = 0.007). Lipid profiles and visceral fat showed insignificant reduction. Conclusions: Flavonoids of MGL attenuates some of the MetS components possibly via reduction in TNF-α inflammatory cytokine.
참고문헌 (35)
- Lau DCW., "New insights in the prevention and early management of type 2 diabetes", Can J Diabetes, 35, pp.239~241 (2011)
- Kaur J., "A comprehensive review on metabolic syndrome", Cardiol Res Pract, 2014, pp.943162~ (2014)
- Janssen I, "Menopause and the metabolic syndrome: the Study of Women’s Health Across the Nation", Arch Intern Med, 168, pp.1568~1575 (2008)
- Arthur FK, "The prevalence of metabolic syndrome and its predominant components among pre-and postmenopausal Ghanaian women", BMC Res Notes, 6, pp.446~ (2013)
- Tawfik SH, "Similar and additive effects of ovariectomy and diabetes on insulin resistance and lipid metabolism", Biochem Res Int, 2015, pp.567945~ (2015)
- Krogh-Madsen R, "Influence of TNF-alpha and IL-6 infusions on insulin sensitivity and expression of IL-18 in humans", Am J Physiol Endocrinol Metab, 291, pp.E108~E114 (2006)
- Jiao K, "Roles of plasma interleukin-6 and tumor necrosis factor-alpha and FFA and TG in the development of insulin resistance induced by high-fat diet", Cytokine, 42, pp.161~169 (2008)
- Luna B, "Oral agents in the management of type 2 diabetes mellitus", Am Fam Physician, 63, pp.1747~1756 (2001)
- Procházková D, "Antioxidant and prooxidant properties of flavonoids", Fitoterapia, 82, pp.513~523 (2011)
- Kasote DM, "Significance of antioxidant potential of plants and its relevance to therapeutic applications", Int J Biol Sci, 11, pp.982~991 (2015)
- Banjarnahor SDS, "Antioxidant properties of flavonoids", Med J Indones, 23, pp.239~244 (2014)
- Hoek-van den Hil EF, "Quercetin induces hepatic lipid omega-oxidation and lowers serum lipid levels in mice", PLoS One, 8, pp.e51588~ (2013)
- Hanhineva K, "Impact of dietary polyphenols on carbohydrate metabolism", Int J Mol Sci, 11, pp.1365~1402 (2010)
- Annadurai T, "Antihyperglycemic and antioxidant effects of a flavanone, naringenin, in streptozotocin-nicotinamideinduced experimental diabetic rats", J Physiol Biochem, 68, pp.307~318 (2012)
- Lopes Galeno DM, "Extract from Eugenia punicifolia is an antioxidant and inhibits enzymes related to metabolic syndrome", Appl Biochem Biotechnol, 172, pp.311~324 (2014)
- Nabavi SF, "The antioxidant activity of wild medlar (Mespilus germanica L.) fruit, stem bark and leaf", Afr J Biotechnol, 10, pp.283~289 (2011)
- Glew RH, "Changes in sugars, organic acids and amino acids in medlar (Mespilus germanica L.) during fruit development and maturation", Food Chem, 83, pp.363~369 (2003)
- Ercisli S, "Phytochemical and antioxidant characteristics of medlar fruits (Mespilus germanica L.)", J Appl Bot Food Qual, 85, pp.86~90 (2012)
- Sun Y, "Black cohosh ameliorates metabolic disorders in female ovariectomized rats", Rejuvenation Res, 19, pp.204~214 (2016)
- Prasannarong M, "Improvements of insulin resistance in ovariectomized rats by a novel phytoestrogen from Curcuma comosa Roxb", BMC Complement Altern Med, 12, pp.28~ (2012)
- Babaei P, "Vitamin D is associated with metabotropic but not neurotrophic effects of exercise in ovariectomized rats", Diabetol Metab Syndr, 9, pp.91~ (2017)
- Ramezani M, "Myricetin protects hippocampal CA3 pyramidal neurons and improves learning and memory impairments in rats with Alzheimer’s disease", Neural Regen Res, 11, pp.1976~1980 (2016)
- Hoseini R, "Vitamin D increases PPARγ expression and promotes beneficial effects of physical activity in metabolic syndrome", Nutrition, 36, pp.54~59 (2017)
- Nounou HA, "Effect of flaxseed supplementation and exercise training on lipid profile, oxidative stress and inflammation in rats with myocardial ischemia", Lipids Health Dis, 11, pp.129~ (2012)
- Bateman LA, "Comparison of aerobic versus resistance exercise training effects on metabolic syndrome (from the Studies of a Targeted Risk Reduction Intervention Through Defined Exercise - STRRIDE-AT", RT), 2011, pp.108, 838~844
- Wegorzewska IN, "Postovariectomy weight gain in female rats is reversed by estrogen receptor alpha agonist, propylpyrazoletriol", Am J Obstet Gynecol, 199, pp.67.e1~61.e5 (2008)
- Yang L, "Total flavonoids extracted from oxytropis falcata bunge improve insulin resistance through regulation on the IKKbeta", NFkappaB inflammatory pathway, 2017, pp.2017, 2405124~
- Li J, "Hypoglycemic and hypolipidemic effects of flavonoids from tatary buckwheat in type 2 diabetic rats", Biomedi Res, 27, pp.132~137 (2016)
- Mahmoud MF, "Quercetin protects against diabetes-induced exaggerated vasoconstriction in rats: effect on low grade inflammation", PLoS One, 8, pp.e63784~ (2013)
- Palacz-Wrobel M, "Effect of apigenin, kaempferol and resveratrol on the gene expression and protein secretion of tumor necrosis factor alpha (TNF-alpha) and interleukin-10 (IL-10) in RAW-264.7 macrophages", Biomed Pharmacother, 93, pp.1205~1212 (2017)
- Al-Numair KS, "Ameliorative effect of kaempferol, a flavonoid, on oxidative stress in streptozotocin-induced diabetic rats", Redox Rep, 20, pp.198~209 (2015)
- Sabio G, "TNF and MAP kinase signalling pathways", Semin Immunol, 26, pp.237~245 (2014)
- Ye J., "Regulation of PPARgamma function by TNF-alpha", Biochem Biophys Res Commun, 374, pp.405~408 (2008)
- Popa C, "The role of TNF-alpha in chronic inflammatory conditions, intermediary metabolism, and cardiovascular risk", J Lipid Res, 48, pp.751~762 (2007)
- 주종길, "Pharmacotherapy for Obesity", 대한폐경학회지, 20(3), pp.91~96 (2014), 대한폐경학회