유두상 갑상선 암에서 Cyclin D1, Cytokeratin 7, Cytokeratin 20, Vimentin의 발현 및 예후 인자와의 연관성에 대한 분석
Expression of Cyclin D1, Cytokeratin 7, Cytokeratin 20 and Vimentin in Papillary Thyroid Cancer and Relationship with Patient’s Prognostic Factors
2
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
Purpose: Cyclin D1, cytokeratin(CK)7, CK20 and vimentin play an important role in tumorigenesis and tumor progression. Our aim was to investigate the expression of cyclin D1, CK7, CK20 and vimentin in 138 papillary thyroid cancer and relationship with clinicobiological factors. We also tried to evaluate the value of those as prognostic factors. Methods: We performed an immunohistochemical assay for cyclin D1, CK7, CK20, and vimentin in 138 papillary thyroid cancer tissue specimens. The correlation between these factors and the clinicobiological parameters was analyzed. Results: In 138 papillary thyroid carcinoma specimens, the expression of cyclin D1, CK7, CK20 and vimentin was seen in 118 cases (85.5%), 133 cases (96.4%), 2 cases (1.4%) and 119 cases (86.2%), respectively. In our cases, there was a significant association among the expressions of cyclin D1, gender and lymph node metastasis. However, no obvious correlation was found between those proteins, age, tumor size, tumor amount, lymph node status and the TNM stage. Conclusion: Our results suggested increased cyclin D1 expression was seen in female gender and it may be a useful marker for evaluating lymph node metastasis. However, the clinical utility of cyclin D1, CK7, CK20 and vimentin in thyroid cancer patients has to be further defined by prospective studies with larger sample sizes.
Abstract
Purpose: Cyclin D1, cytokeratin(CK)7, CK20 and vimentin play an important role in tumorigenesis and tumor progression. Our aim was to investigate the expression of cyclin D1, CK7, CK20 and vimentin in 138 papillary thyroid cancer and relationship with clinicobiological factors. We also tried to evaluate the value of those as prognostic factors. Methods: We performed an immunohistochemical assay for cyclin D1, CK7, CK20, and vimentin in 138 papillary thyroid cancer tissue specimens. The correlation between these factors and the clinicobiological parameters was analyzed. Results: In 138 papillary thyroid carcinoma specimens, the expression of cyclin D1, CK7, CK20 and vimentin was seen in 118 cases (85.5%), 133 cases (96.4%), 2 cases (1.4%) and 119 cases (86.2%), respectively. In our cases, there was a significant association among the expressions of cyclin D1, gender and lymph node metastasis. However, no obvious correlation was found between those proteins, age, tumor size, tumor amount, lymph node status and the TNM stage. Conclusion: Our results suggested increased cyclin D1 expression was seen in female gender and it may be a useful marker for evaluating lymph node metastasis. However, the clinical utility of cyclin D1, CK7, CK20 and vimentin in thyroid cancer patients has to be further defined by prospective studies with larger sample sizes.
참고문헌 (24)
- Kang YK, "Expression of G1-S modulators (p53, p16, p27, cyclin D1, Rb) and Smad4", Dpc4 in intrahepatic cholangiocarcinoma, 2002, pp.33, 877~883
- Gillett C, "Amplification and overexpression of cyclin D1 in breast cancer detected by immunohistochemical staining", Cancer Res, 54, pp.1812~1817 (1994)
- Lee SH, "Expression of cell-cycle regulators (cyclin D1, cyclin E, p27kip1, p57kip2) in papillary thyroid carcinoma", Otolaryngol Head Neck Surg, 142, pp.332~337 (2010)
- Lantsov D, "Cyclin D1 overexpression in thyroid papillary microcarcinoma: its association with tumour size and aberrant beta-catenin expression", Histopathology, 47, pp.248~256 (2005)
- Sun TT, "Keratin cytoskeletons in epithelial cells of internal organs", Proc Natl Acad Sci USA, 76, pp.2813~2817 (1979)
- Fuchs E, "Regulation of terminal differentiation of cultured human keratinocytes by vitamin A", Cell, 25, pp.617~625 (1981)
- Blumenberg M, "Regulation of keratin gene expression: the role of the nuclear receptors for retinoic acid, thyroid hormone, and vitamin D3", J Invest Dermatol, 98(6), pp.42S~49S (1992)
- 이주호, "갑상샘 종양에서 p53, cyclin D1 및 c-myc 발현의 의의", The Korean Journal of Pathology, 38(1), pp.29~34 (2004), 대한병리학회
- Wang S, "The role of cell cycle regulatory protein, cyclin D1, in the progression of thyroid cancer", Mod Pathol, 13, pp.882~887 (2000)
- Goto A, "An immunohistochemical analysis of cyclin D1, p53, and p21waf1", cip1 proteins in tumors originating from the follicular epithelium of the thyroid gland, 2001, pp.197, 217~222
- Khoo ML, "Overexpression of cyclin D1 and underexpression of p27 predict lymph node metastases in papillary thyroid carcinoma", J Clin Endocrinol Metab, 87, pp.1814~1818 (2002)
- Temmim L, "Cyclin D1 protein expression in human thyroid gland and thyroid cancer", Anat Histol Embryol, 35, pp.125~129 (2006)
- 박성진, "유두상 갑상선 암 환자에서 Cyclin D1, Vimentin과 Thyroglobulin의 발현과 임상적 예후 인자의 상관성 분석", 대한외과학회지, 70(6), pp.425~429 (2006), 대한외과학회
- Campbell F, "Application of cytokeratin 7 and 20 immunohistochemistry to diagnostic pathology", Current Diagnostic Pathology, 7, pp.113~122 (2001)
- Fonseca E, "Pattern of expression of intermediate cytokeratin filaments in the thyroid gland: an immunohistochemical study of simple and stratified epithelial-type cytokeratins", Virchows Arch, 430, pp.239~245 (1997)
- Bejarano PA, "Thyroid transcription factor-1, thyroglobulin, cytokeratin 7, and cytokeratin 20 in thyroid neoplasms", Appl Immunohistochem Mol Morphol, 9, pp.189~194 (2000)
- Lam KY, "Cytokeratin expression profiles in thyroid carcinomas", Eur J Surg Oncol, 27, pp.631~635 (2001)
- Cameron BR, "Cytokeratin subtypes in thyroid tumours: immunohistochemical study with emphasis on the follicular variant of papillary carcinoma", J Otolaryngol, 32, pp.319~322 (2003)
- Schmitz-Winnenthal FH, "Detection and prognostic relevance of cytokeratin 20 in differentiated and anaplastic thyroid carcinomas by RT-PCR", Surgery, 134, pp.964~971 (2003)
- McInroy L, "Down-regulation of vimentin expression inhibits carcinoma cell migration and adhesion", Biochem Biophys Res Commun, 360, pp.109~114 (2007)
- Bennett GS, "Redistribution of intermediate filament subunits during skeletal myogenesis and maturation in vitro", J Cell Biol, 82, pp.577~584 (1979)
- Vasko V, "Gene expression and functional evidence of epithelial-to-mesenchymal transition in papillary thyroid carcinoma invasion", Proc Natl Acad Sci USA, 104, pp.2803~2808 (2007)
- Yamamoto Y, "An immunohistochemical study of epithelial membrane antigen, cytokeratin, and vimentin in papillary thyroid carcinoma. Recognition of lethal and favorable prognostic types", Cancer, 70, pp.2326~2333 (1992)
- Yagi Y, "The localization of cytoskeletal proteins and thyroglobulin in thyroid microcarcinoma in comparison with clinically manifested thyroid carcinoma", Cancer, 56, pp.1967~1971 (1985)