Identification, Enzymatic Activity, and Decay Ability of Basidiomycetous Fungi Isolated from the Decayed Bark of Mongolian Oak (Quercus mongolica Fisch. ex Ledeb.)
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Decay fungi can decompose plant debris to recycle carbon in the ecosystem. Still, they can also be fungal pathogens, which can damage living trees and/or wood material and cause a large amount of timber loss. We isolated and identified basidiomycetous fungi from the decayed bark of Mongolian oak wrapped with sticky roll traps. The degrading enzyme activities were then tested for all fungal isolates. The decay ability of selected isolates was assessed based on the weight loss of wood discs after inoculating with culture suspension of decay fungi under the different humidity levels. A total of 46 basidiomycetous fungal isolates belonged to 12 species, and 10 genera were obtained from Jong Myo (16 isolates), Chang Kyung palace (7 isolates), Cheong Gye (10 isolates), and Gun Po (13 isolates). Gymnopus luxurians was the most dominant fungus in the present study, and this species distributed in all survey sites with 9 isolates in Jong Myo, followed by 3 isolates in Chang Kyung palace, while Cheong Gye and Gun Po had only 1 isolate each. Among 46 isolates, 44 isolates secreted at least one enzyme, while 25 isolates produced both cellulase and phenol oxidase enzymes, and 2 isolates produced neither. The assessment of decay ability by artificial inoculation indicated that the weight loss of wood discs was significantly influenced by humidity conditions when inoculated with bark decay fungi. The percent weight losses by G. luxurians inoculation in RH of 90-100% and RH of 65-75% were 4.61% and 2.45%, respectively. The weight loss caused by Abortiporus biennis were 6.67% and 0.46% in RH of 90-100% and RH of 45-55%, respectively. The humidity reduction approach should be applied for further studies to control the growth and spread of bark decay fungi on the trunks wrapped with sticky roll traps.
Abstract
Decay fungi can decompose plant debris to recycle carbon in the ecosystem. Still, they can also be fungal pathogens, which can damage living trees and/or wood material and cause a large amount of timber loss. We isolated and identified basidiomycetous fungi from the decayed bark of Mongolian oak wrapped with sticky roll traps. The degrading enzyme activities were then tested for all fungal isolates. The decay ability of selected isolates was assessed based on the weight loss of wood discs after inoculating with culture suspension of decay fungi under the different humidity levels. A total of 46 basidiomycetous fungal isolates belonged to 12 species, and 10 genera were obtained from Jong Myo (16 isolates), Chang Kyung palace (7 isolates), Cheong Gye (10 isolates), and Gun Po (13 isolates). Gymnopus luxurians was the most dominant fungus in the present study, and this species distributed in all survey sites with 9 isolates in Jong Myo, followed by 3 isolates in Chang Kyung palace, while Cheong Gye and Gun Po had only 1 isolate each. Among 46 isolates, 44 isolates secreted at least one enzyme, while 25 isolates produced both cellulase and phenol oxidase enzymes, and 2 isolates produced neither. The assessment of decay ability by artificial inoculation indicated that the weight loss of wood discs was significantly influenced by humidity conditions when inoculated with bark decay fungi. The percent weight losses by G. luxurians inoculation in RH of 90-100% and RH of 65-75% were 4.61% and 2.45%, respectively. The weight loss caused by Abortiporus biennis were 6.67% and 0.46% in RH of 90-100% and RH of 45-55%, respectively. The humidity reduction approach should be applied for further studies to control the growth and spread of bark decay fungi on the trunks wrapped with sticky roll traps.
참고문헌 (34)
- Anastasi A, "Fungi as Bioremediators", pp.29~49 (2013), Springer
- Bold G, "The Protective Role of Bark and Bark Fibers of the Giant Sequoia(Sequoiadendron giganteum)during High-Energy Impacts", Int J Mol Sci, 21, pp.3355~ (2020)
- Brischke C, "Changes in Sorption and Electrical Properties of Wood Caused by Fungal Decay", Holzforschung, 73, pp.445~455 (2019)
- Brischke C, "Performance of Bio-based Building Materials", pp.187~247 (2017), Woodhead Publishing
- Ding S, "Diversity, Abundance, and Distribution of Wood-Decay Fungi in Major Parks of Hong Kong", Forests, 11, pp.1030~ (2020)
- Futai K, "Pine Wood Nematode, Bursaphelenchus xylophilus", Annu Rev Phytopathol, 51, pp.61~83 (2013)
- Glaeser JA, "Decay Fungi of Oaks and Associated Hardwoods for Western Arborists", Western Arborist, 2010, pp.32~46 (2010)
- A Reum Hong, "Screening of Antifungal Microorganisms with Strong Biological Activity against Oak Wilt Fungus, Raffaelea quercus-mongolicae", Journal of forest and environmental science, 34(5), pp.395~404 (2018), 산림과학연구소[DOI]
- Hopkins AJM, "The taxonomy and ecology of wood decay fungi in Eucalyptus obliqua trees and logs in the wet sclerophyll forests of southern Tasmania", PhD, pp.2007
- Jang Y, "Perenniporia koreana, a New Wood-Rotting Basidiomycete from South Korea", Mycotaxon, 130, pp.173~179 (2015)
- Su Jung Jung, "Screening and Evaluation of Streptomyces Species as a Potential Biocontrol Agent against a Wood Decay Fungus, Gloeophyllum trabeum", Mycobiology, 46(2), pp.138~146 (2018), 한국균학회
- Kim KH, "Raffaelea quercus-mongolicae sp. nov. Associated with Platypus koryoensis on oak in Korea", Mycotaxon, 110, pp.189~197 (2009)
- 김남규, "Diversity and Distribution of Wood Decay Fungi in Korea", Journal of forest and environmental science, 34(2), pp.126~135 (2018), 산림과학연구소[DOI]
- Kim NK, "Genetic diversity, wood decaying patterns and biological control of Trametes versicolor isolates", PhD, pp.2014
- Lee DH, "Investigation of the Mating-type Distribution of Raffaelea quercus-mongolicae in South Korea", For Pathol, 50, pp.e12590~ (2020)
- 이진흥, "Prevention of Oak Wilt by Tree Injection of Culture Suspension of an Antifungal Microorganism, Streptomyces blastmyceticus against Oak Wilt Fungus, Raffaelea quercus-mongolicae", Journal of forest and environmental science, 34(5), pp.376~381 (2018), 산림과학연구소[DOI]
- Lee JK, "Screening of White Rot Fungi with Selective Delignification Capacity for Biopulping", Korean J Mycol, 26, pp.144~152 (1998)
- Brief Description of Wood Decay and the Common Decay Fungi on Shade Trees in Urban Areas, "Lee JK.", Tree Diagnostic Center, Kangwon National University, 32~ (2020)
- Lindgren BS, "Evolution of Tree Killing in Bark Beetles(Coleoptera : Curculionidae) : Trade-offs between the Maddening Crowds and a Sticky Situation", Can Entomol, 145, pp.471~495 (2013)
- Mallerman J, "Biodegradation of Nonylphenol Polyethoxylates by Litter-basidiomycetous Fungi", J Environ Chem Eng, 7, pp.103316~ (2019)
- A review of the role of fungi in wood decay of forest ecosystems, "Marcot BG.", USDA For. Ser. Pacific, 31~, pp.Res. Note. PNW-RN-575 (2017)
- Nguyen Manh Ha, "Screening of Endophytic Fungal Isolates Against Raffaelea quercus-mongolicae Causing Oak Wilt Disease in Korea", Mycobiology, 48(6), pp.484~494 (2020), 한국균학회[DOI]
- Oguro M, "Mortality Due to Japanese Oak Wilt Disease and Surrounding Forest Compositions", Data Brief, 5, pp.208~212 (2015)
- Pournou A., "Biodeterioration of Wooden Cultural Heritage: Organisms and Decay Mechanisms in Aquatic and Terrestrial Ecosystems", pp.177~260 (2020), Springer
- Saitta A, "Biodiversity of Wood-Decay Fungi in Italy", Plant Biosyst, 145, pp.958~968 (2011)
- Schmidt O, "Wood and Tree Fungi: Biology, Damage, Protection, and Use" (2006), Springer
- Schweingruber FH, "Bark Anatomy of Trees and Shrubs in the Temperate Northern Hemisphere" (2019), Springer International Publishing
- Srivastava S, "Wood Decaying Fungi" (2013), LAP Lambert Academic Publishing
- Thompson TA, "A study of basidiomycetes isolated from coarse woody debris and contiguous soil horizons in a mixed deciduous-conifer forest in New Hampshire, United States of America", PhD, pp.2004
- Tittiger C, "Pine Bark Beetles" (2016), Academic Press
- Vega FE, "Bark Beetles: Biology and Ecology of Native and Invasive Species" (2015), Academic Press
- Wang J, "A Review of the Long-term Effects of Humidity on the Mechanical Properties of Wood and Wood-based Products", Eur J Wood Prod (2020)[DOI]
- 이수희, "In vivo Pathogenicity Test of Oak Wilt Fungus (Raffaelea quercus-mongolicae) on Oriental Chestnut Oak (Quercus acutissima)", Journal of forest and environmental science, 33(4), pp.342~347 (2017), 산림과학연구소
- Zabel RA, "Wood Microbiology: Decay and Its Prevention", pp.99~128 (2020), Academic Press