중이 진주종에서 p53 단백 및 p53 유전자의 발현양상
Expression of p53 Protein and Gene in Human Cholesteatoma
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Abstract
Background and Objectives：Migration, hyperproliferation, diferentiation of basal keratinocytes and accumulation of keratin debris are the major pathogenic reactions in midle ear cholesteatoma. p53 is a multi-functional protein that acts as a negative e-ver, it is still not proven whether overexpresion of p53 is associated with the increase in mRNA or modifications at the protein level. Materials and Method：Twenty-seven human cholesteatoma and 20 retroauricular skins were obtained. We tried to detect the overexpresion of p53 using imunohistochemical staining, and conducted semi-quantitative analysis of mRNA level using relative reverse transcription polymerase chain reaction (RT-PCR). Results：p53 stained cells betwen cholesteatoma and normal skin. However, we could not find any significant diferences in the p53 mRNA expression between them. Conclusion：We could confirm the overexpresion of p53 in human cholesteatoma, which does not sem to be related with transcriptional up-regulation.
참고문헌 (19)
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