담배 연기에 노출된 백서에서 후각 상피의 조직학적 변화
Histopathological Changes of Olfactory Epitheliumin the Rats Exposed to Cigarette Smoke
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Abstract
Background and Objectives：tion, the pathophysiology is porly understood. The purpose of this study is to evaluate the histopathological change of olfactory epithelium according to exposure duration of cigarete smoke and to investigate the efects of cigarete smoke on the olfactory epithelium. Subjects and Method：Thirty-six healthy Sprague-Dawley rats were divided into 2 groups. Experimental groups (n= 28) were exposed to cigarete smoke during 2.5 hours (total 5 cigarettes) per one day for 5 days, 11 days and 3, 4, 5, 6, 9 (n= 8) was placed at the same room without smoke exposure and sacrificed at 4 and 9 weeks. Histopathol-ogical changes of olfactory epithelium through light microscope and imunohistochemistric findings of olfactory marker protein (OMP) through confocal microscope were observed. The numbers of cels in olfactory epithelium were counted at each period. Results：From 5 days of cigarete smoke exposure, defection of epithelial cels, random arrangement of olfactory receptor eased at 11 days and 3 weeks after smoke exposure, and this finding of decreased number of olfactory receptor cels were continued until 9 weeks of exposure. Numbers of OMP-positive olfactory receptor cels were continuously decreased according to exposure duration. Conclusion：The olfactory epithelial cells could be directly afected by cigarete smoke. The numbers of olfactory epithelial cells including olfactory receptor cells were continuously decreased until 9 weks after cigarette smoke exposure. (Korean J Otorhinolaryngol-Head Neck Surg 2007;50:766-72)
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