두경부편평세포암에서 Human Papillomavirus 감염 여부에 대한 선별검사로서의 p16의 유용성
The Usability of p16 Immunohistochemical Staining as a Screening Test of Human Papillomavirus Infection in Head and Neck Squamous Cell Carcinoma
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Background and Objectives Although human papillomavirus (HPV) infection has been recognized as having an important role in the pathogenesis of head and neck squamous cell carcinoma (HNSCC), there is no simple and reliable screening test of HPV infection in HNSCC to date. In the present study, we investigated the usability of p16 immunohistochemical staining as a screening test for HPV infection in HNSCC. Subjects and Method Paraffin blocks were obtained from 45 tonsil SCC patients, who underwent surgery as an initial treatment between 1995 and 2004. Paraffin blocks were also obtained from 20 normal controls. Expression of p16 was investigated immunohistochemically and these results were compared with results of HPV genotyping and the real-time polymerase chain reaction (PCR) assay of viral oncoprotein to validate p16 staining as a reliable diagnostic tool of HPV infection. ResultsZZThe expression of p16 was found to be significantly different between tonsil SCC (32/45, 71.1%) and normal control tissues (0/20, 0%)(p＜0.05). Among the 45 patients, HPV genotyping and real-time PCR assay were performed in 21 of the 32 p16 (+) patients and in 6 patients of the 13 p16 (-) patients. HPV-16 was detected in 18 (85.7%) out of the 21 p16 (+) patients and in 2 (33.3%) out of the 6 p16 (-) patients. The p16 expression and HPV-16 status was significantly correlated (p＜0.05). The two patients who had HPV-16 infection but did not have p16 expression showed an episomal infection or very low viral copy number each. Conclusion The p16 expression may be an useful marker of HPV infection in HNSCC and may be used as a screening test for HPV infection in HNSCC. Korean J Otorhinolaryngol-Head Neck Surg 2010;53:620-6
Abstract
Background and Objectives Although human papillomavirus (HPV) infection has been recognized as having an important role in the pathogenesis of head and neck squamous cell carcinoma (HNSCC), there is no simple and reliable screening test of HPV infection in HNSCC to date. In the present study, we investigated the usability of p16 immunohistochemical staining as a screening test for HPV infection in HNSCC. Subjects and Method Paraffin blocks were obtained from 45 tonsil SCC patients, who underwent surgery as an initial treatment between 1995 and 2004. Paraffin blocks were also obtained from 20 normal controls. Expression of p16 was investigated immunohistochemically and these results were compared with results of HPV genotyping and the real-time polymerase chain reaction (PCR) assay of viral oncoprotein to validate p16 staining as a reliable diagnostic tool of HPV infection. ResultsZZThe expression of p16 was found to be significantly different between tonsil SCC (32/45, 71.1%) and normal control tissues (0/20, 0%)(p＜0.05). Among the 45 patients, HPV genotyping and real-time PCR assay were performed in 21 of the 32 p16 (+) patients and in 6 patients of the 13 p16 (-) patients. HPV-16 was detected in 18 (85.7%) out of the 21 p16 (+) patients and in 2 (33.3%) out of the 6 p16 (-) patients. The p16 expression and HPV-16 status was significantly correlated (p＜0.05). The two patients who had HPV-16 infection but did not have p16 expression showed an episomal infection or very low viral copy number each. Conclusion The p16 expression may be an useful marker of HPV infection in HNSCC and may be used as a screening test for HPV infection in HNSCC. Korean J Otorhinolaryngol-Head Neck Surg 2010;53:620-6
참고문헌 (19)
- Frisch M, "Changing patterns of tonsillar squamous cell carcinoma in the United States", Cancer Causes Control, 6, pp.489~495 (2000)
- Kuo KT, "The biomarkers of human papillomavirus infection in tonsillar squamous cell carcinoma-molecular basis and predicting favorable outcome", Mod Pathol, 21(4), pp.376~386 (2008)
- Fakhry C, "Clinical implications of human papillomavirus in head and neck cancers", J Clin Oncol, 24(17), pp.2606~2611 (2006)
- Lee WT, "Use of in situ hybridization to detect human papillomavirus in head and neck squamous cell carcinoma patients without a history of alcohol or tobacco use", Arch Pathol Lab Med, 132(10), pp.1653~1656 (2008)
- Shibata D, "Detection of human papillomavirus in normal and dysplastic tissue by the polymerase chain reaction", Lab Invest, 59(4), pp.555~559 (1988)
- Brandstetter T, "A polymer-based DNA biochip platform for human papilloma virus genotyping", J Virol Methods, 163(1), pp.40~48 (2010)
- Klussmann JP, "Expression of p16 protein identifies a distinct entity of tonsillar carcinomas associated with human papillomavirus", Am J Pathol, 162(3), pp.747~753 (2003)
- Klaes R, "Overexpression of p16 (INK4A) as a specific marker for dysplastic and neoplastic epithelial cells of the cervix uteri", Int J Cancer, 92(2), pp.276~284 (2001)
- Syrjänen S, "Human papillomavirus (HPV) in head and neck cancer", J Clin Virol, 32, pp.S59~S66 (2005)
- Gillison ML, "Human papillomavirus-associated head and neck squamous cell carcinoma: mounting evidence for an etiologic role for human papillomavirus in a subset of head and neck cancers", Curr Opin Oncol, 13(3), pp.183~188 (2001)
- Herrero R, "Human papillomavirus and oral cancer: the International Agency for Research on Cancer multicenter study", J Natl Cancer Inst, 95(23), pp.1772~1783 (2003)
- Begum S, "Tissue distribution of human papillomavirus16 DNA integration in patients with tonsillar carcinoma", Clin Cancer Res, 11(16), pp.5694~5699 (2005)
- Gillison ML, "Human papillomavirus and prognosis of oropharyngeal squamouscell carcinoma: implications for clinical research in head and neck cancers", J Clin Oncol, 24(36), pp.5623~5625 (2006)
- Kleter B, "Development and clinical evaluation of a highly sensitive PCR-reverse hybridization line probe assay for detection and identification of anogenital human papillomavirus", J Clin Microbiol, 37(8), pp.2508~2517 (1999)
- Lörincz AT, "Molecular methods for the detection of human papillomavirus infection", Obstet Gynecol Clin North Am, 23(3), pp.707~730 (1996)
- Ha PK, "Real-time quantitative PCR demonstrates low prevalence of human papillomavirus type 16 in premalignant and malignant lesions of the oral cavity", Clin Cancer Res, 8(5), pp.1203~1209 (2002)
- Gillison ML, "Evidence for a casual association between human papillomavirus and a subset of head and neck cancers", J Natl Cancer Inst, 92(9), pp.709~720 (2000)
- Begum S, "Detection of human papillomavirus in cervical lymph nodes: a highly effective strategy for localizing site of tumor origin", Clin Cancer Res, 9(17), pp.6469~6475 (2003)
- Mellin H, "Human papillomavirus type 16 is episomal and a high viral load may be correlated to better prognosis in tonsillar cancer", Int J Cancer, 102(2), pp.152~158 (2002)