The Correlation of Tissue’s Endotype Biomarkers and Dominant Inflammation Cell in Chronic Rhinosinusitis
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Background and Objectives This study analyzes the relationship between endotype bio-markers and the type of tissue inflammation in chronic rhinosinusitis (CRS) in order to obtaina more accurate division of disease groups and appropriate therapy. Subjects and Method We carried out a cross-sectional study involving 33 samples of CRSpatients. We conducted enzyme-linked immunosorbent assay to examine endotype biomark-ers and histopathology methods to examine tissue inflammation. Results Statistical analysis with an independent t-test showed significant differences withrespect to eosinophil cationic protein (ECP) between the eosinophilic and neutrophilic groups( p<0.05), whereas there were no significant differences between the two groups ( p>0.05) withrespect to Charcot-Leyden crystal (CLC), interleukin-5 (IL-5), interferon gamma (IFN-γ), in-terleukin-17A (IL-17A), and oncostatin M (OSM). The Spearman test showed also showed thatECP, CLC, IL-5, IL-17A, IFN-γ and OSM had no significant correlation with the two groups( p>0.05). The receiver operating characteristics analysis showed the optimal cut-off value ofECP of 342.22 pg/mL with sensitivity and specificity of 72.7% for determining the inflamma-tion type (area under cover=0.78, p=0.009). Conclusion The higher level of ECP was more likely to be found in the eosinophilic tissueinflammation. Consequently, we propose using ECP as the main biomarker to identify the CRStype 2. Further research with a larger sample size is required for cut-off points of ECP in orderto determine the endotype of CRS.
Abstract
Background and Objectives This study analyzes the relationship between endotype bio-markers and the type of tissue inflammation in chronic rhinosinusitis (CRS) in order to obtaina more accurate division of disease groups and appropriate therapy. Subjects and Method We carried out a cross-sectional study involving 33 samples of CRSpatients. We conducted enzyme-linked immunosorbent assay to examine endotype biomark-ers and histopathology methods to examine tissue inflammation. Results Statistical analysis with an independent t-test showed significant differences withrespect to eosinophil cationic protein (ECP) between the eosinophilic and neutrophilic groups( p<0.05), whereas there were no significant differences between the two groups ( p>0.05) withrespect to Charcot-Leyden crystal (CLC), interleukin-5 (IL-5), interferon gamma (IFN-γ), in-terleukin-17A (IL-17A), and oncostatin M (OSM). The Spearman test showed also showed thatECP, CLC, IL-5, IL-17A, IFN-γ and OSM had no significant correlation with the two groups( p>0.05). The receiver operating characteristics analysis showed the optimal cut-off value ofECP of 342.22 pg/mL with sensitivity and specificity of 72.7% for determining the inflamma-tion type (area under cover=0.78, p=0.009). Conclusion The higher level of ECP was more likely to be found in the eosinophilic tissueinflammation. Consequently, we propose using ECP as the main biomarker to identify the CRStype 2. Further research with a larger sample size is required for cut-off points of ECP in orderto determine the endotype of CRS.
참고문헌 (26)
- Rudmik L, "Economics of chronic rhinosinusitis", Curr Allergy Asthma Rep, 17(4), pp.20~ (2017)
- Delemarre T, "Rethinking neutrophils and eosinophils in chronic rhinosinusitis", J Allergy Clin Immunol, 148(2), pp.327~335 (2021)
- Talat R, "Chronic rhinosinusitis patients with and without polyps experience different symptom perception and quality of life burdens", Am J Rhinol Allergy, 34(6), pp.742~750 (2020)
- Kucuksezer UC, "Chronic rhinosinusitis: Pathogenesis, therapy options, and more", Expert Opin Pharmacother, 19(16), pp.1805~1815 (2018)
- Liu C, "Predictive significance of Charcot–Leyden crystals for eosinophilic chronic rhinosinusitis with nasal polyps", Am J Rhinol Allergy, 33(6), pp.671~680 (2019)
- Xu X, "Novel findings in immunopathophysiology of chronic rhinosinusitis and their role in a model of precision medicine", Allergy, 75(4), pp.769~780 (2020)
- Huriyati E, "Differences in expression of inflammatory mediator in mucosal and polyp tissue between chronic rhinosinusitis and recurrent chronic rhinosinusitis", Open Access Maced J Med Sci, 7(11), pp.1733~1738 (2019)
- Ahern S, "Inflammation and endotyping in chronic rhinosinusitis—a paradigm shift", Medicina(Kaunas), 55(4), pp.95~ (2019)
- Wicaksana DA, "Correlation between β-glucan and neutrophil", eosinophil ratio of paranasal sinus mucosa and blood in the diagnosis of chronic fungal rhinosinusitis, 2018, pp.33(5), 393~400
- Brescia G, "Sex and age-related differences in chronic rhinosinusitis with nasal polyps electing ESS", Am J Otolaryngol, 43(2), pp.103342~ (2022)
- Kato A, "Endotypes of chronic rhinosinusitis: Relationships to disease phenotypes, pathogenesis, clinical findings, and treatment approaches", Allergy, 77(3), pp.812~826 (2022)
- Fokkens WJ, "Executive summary of EPOS 2020 including integrated care pathways", Rhinology, 58(2), pp.82~111 (2020)
- Yang LY, "Vγ1+γδT cells are correlated with increasing expression of eosinophil cationic protein and metalloproteinase-7 in chronic rhinosinusitis with nasal polyps inducing the formation of edema", Allergy Asthma Immunol Res, 9(2), pp.142~151 (2017)
- Bachert C, "Endotype-driven care pathways in patients with chronic rhinosinusitis", J Allergy Clin Immunol, 141(5), pp.1543~1551 (2018)
- Stevens WW, "Cytokines in chronic rhinosinusitis. Role in eosinophilia and aspirin-exacerbated respiratory disease", Am J Respir Crit Care Med, 192(6), pp.682~694 (2015)
- Lou H, "Highlights of eosinophilic chronic rhinosinusitis with nasal polyps in definition, prognosis, and advancement", Int Forum Allergy Rhinol, 8(11), pp.1218~1225 (2018)
- Tan BK, "Heterogeneous inflammatory patterns in chronic rhinosinusitis without nasal polyps in Chicago, Illinois", J Allergy Clin Immunol, 139(2), pp.699~703 (2017)
- Delemarre T, "A substantial neutrophilic inflammation as regular part of severe type 2 chronic rhinosinusitis with nasal polyps", J Allergy Clin Immunol, 147(1), pp.179~188 (2021)
- Hoggard M, "Inflammatory endotypes and microbial associations in chronic rhinosinusitis", Front Immunol, 9, pp.2065~ (2018)
- Cho SH, "Agerelated increased prevalence of asthma and nasal polyps in chronic rhinosinusitis and its association with altered IL-6 trans-signaling", Am J Respir Cell Mol Biol, 53(5), pp.601~606 (2015)
- Kim DK, "Elastase-positive neutrophils are associated with refractoriness of chronic rhinosinusitis with nasal polyps in an Asian population", Allergy Asthma Immunol Res, 12(1), pp.42~55 (2020)
- Cao PP, "Pathophysiologic mechanisms of chronic rhinosinusitis and their roles in emerging disease endotypes", Ann Allergy Asthma Immunol, 122(1), pp.33~40 (2019)
- Wang X, "Diversity of TH cytokine profiles in patients with chronic rhinosinusitis: A multicenter study in Europe, Asia, and Oceania", J Allergy Clin Immunol, 138(5), pp.1344~1353 (2016)
- Pothoven KL, "Oncostatin M promotes mucosal epithelial barrier dysfunction, and its expression is increased in patients with eosinophilic mucosal disease", J Allergy Clin Immunol, 136(3), pp.737~746 (2015)
- Tian T, "H3N2 influenza virus infection enhances oncostatin M expression in human nasal epithelium", Exp Cell Res, 371(2), pp.322~329 (2018)
- Poposki JA, "Elevation of activated neutrophils in chronic rhinosinusitis with nasal polyps", J Allergy Clin Immunol, 149(5), pp.1666~1674 (2022)