지질다당류가 비강상피세포의 Aspergillus fumigatus 생물막 형성에 미치는 영향
Effect of Lipopolysaccharide on Development of Aspergillus fumigatus Biofilm on Nasal Epithelial Cells
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Background and Objectives Aspergillus fumigatus is one of the common causes of fungalairway inflammatory diseases and lipopolysaccharide (LPS) acts as key regulator of airwayinflammation. In addition, bacterial and fungal biofilm commonly coexist in chronic rhinosi-nusitis. In this study, we evaluated the effect of LPS on the development of A. fumigatus bio-film formation on the nasal epithelial cells. Materials and Method Primary nasal epithelial cells were cultured with A. fumigatus co-nidia with or without LPS for 5 days. The production of interleukin (IL)-6, IL-8, and trans-forming growth factor (TGF)-β1 from nasal epithelial cells was determined by enzyme-linkedimmunosorbent assay. The effects of LPS on A. fumigatus biofilm formation were determinedusing biofilm dry weight, and crystal violet, concanavalin A, safranin staining, and confocalscanning laser microscopy. Results LPS and A. fumigatus significantly enhanced the production of IL-6, IL-8, andTGF-β1 from nasal epithelial cells. A. fumigatus can form biofilm on primary nasal epithelialcells, and this significantly increased in a time-dependent manner when cocultured with LPS,the dry weight, concanavalin A, and safranin staining. Conclusion The exposure of A. fumigatus to LPS enhanced the formation of biofilms. Thecoexistence of LPS and A. fumigatus enhanced fungal biofilm formation and this may be as-sociated with the development of recalcitrant airway inflammatory diseases.
Abstract
Background and Objectives Aspergillus fumigatus is one of the common causes of fungalairway inflammatory diseases and lipopolysaccharide (LPS) acts as key regulator of airwayinflammation. In addition, bacterial and fungal biofilm commonly coexist in chronic rhinosi-nusitis. In this study, we evaluated the effect of LPS on the development of A. fumigatus bio-film formation on the nasal epithelial cells. Materials and Method Primary nasal epithelial cells were cultured with A. fumigatus co-nidia with or without LPS for 5 days. The production of interleukin (IL)-6, IL-8, and trans-forming growth factor (TGF)-β1 from nasal epithelial cells was determined by enzyme-linkedimmunosorbent assay. The effects of LPS on A. fumigatus biofilm formation were determinedusing biofilm dry weight, and crystal violet, concanavalin A, safranin staining, and confocalscanning laser microscopy. Results LPS and A. fumigatus significantly enhanced the production of IL-6, IL-8, andTGF-β1 from nasal epithelial cells. A. fumigatus can form biofilm on primary nasal epithelialcells, and this significantly increased in a time-dependent manner when cocultured with LPS,the dry weight, concanavalin A, and safranin staining. Conclusion The exposure of A. fumigatus to LPS enhanced the formation of biofilms. Thecoexistence of LPS and A. fumigatus enhanced fungal biofilm formation and this may be as-sociated with the development of recalcitrant airway inflammatory diseases.
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