국내 매독검사의 외부신빙도조사 현황
Current Status of External Quality Assessment of Syphilis Test in Korea
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Abstract
Background : Current status of external quality assessment (EQA) of laboratory tests for syphilis in Korea was analyzed to find out the problems that should be improved in the future. Methods : Based on the data from the external quality assessment program performed twice a year by the Immunoserology Subcommittee of the Korean Association of Quality Assurance for Clinical Laboratory from the year 2004 to 2006, discordance rates were analyzed according to the test method and commercial kit used. Results : Among the laboratories participating in the EQA program for syphilis test, about 90% of them used non-treponemal tests and about 55% treponemal tests. The non-treponemal tests included RPR (rapid plasma reagin) and VDRL tests used in 88% (363/412) and 11% (45/412), respectively, of the laboratories. The discordance rates were 2.2% for RPR test and 3.6% for VDRL. For the treponemal tests, Treponema pallidum hemagglutination assay (TPHA) was used in 60-76% and Immunochromatography assay (ICA) in about 30% of the laboratories in 2006. A high discordance rate of over 10% was reported in both TPHA and in ICA methods, possibly due to a low titer (1:1 in VDRL) of EQA samples in 2005. Analysis of the accumulated data from year 2004 to 2006 showed that the discordance rates of TPHA, ICA, and FTA-ABS were 4.6%, 3.7%, and 2.7%, respectively. Conclusions : For syphilis tests, RPR test, TPHA, and ICA are mainly used in Korea. A high discordance rate is still reported in TPHA and ICA, especially when testing samples with a low titer. Further analysis of data and education of laboratory personnel are needed for the improvement of the EQA program. (Korean J Lab Med 2008;28:207-13)
참고문헌 (21)
- Peterman TA, "The changing epidemiology of syphilis", Sex Transm Dis, 32(S), pp.S4~S10 (2005)
- Larsen SA, "Laboratory diagnosis and interpretation of tests for syphilis", Clin Microbiol Rev, 8, pp.1~21 (1995)
- Hsu WS, "Quality assurance in clinical laboratories in Taiwan", J Formos Med Assoc, 99, pp.235~242 (2000)
- Neimeister RP, "Proficiency testing, trouble shooting and quality control for the RPR test", Am J Med Technol, 41, pp.13~17 (1975)
- Snell JJ, "The United Kingdom national microbiological quality assessment scheme", J Clin Pathol, 35, pp.82~93 (1982)
- Taylor RN, "Center for Disease Control Diagnostic Immunology Proficiency Testing Program results for 1977", J Clin Microbiol, 8, pp.388~395 (1978)
- Taylor RN, "Assessment of laboratory improvement by the Center for Disease Control Diagnostic Immunology Proficiency Testing Program", J Clin Microbiol, 13, pp.356~368 (1981)
- Muller I, "Is serological testing a reliable tool in laboratory diagnosis of syphilis? Meta-analysis of eight external quality control surveys performed by the German infection serology proficiency testing program", J Clin Microbiol, 44, pp.1335~1341 (2006)
- Cha YJ, "Annual report on external quality assessment in immunoserology in Korea", J Lab Med Qual, 26, pp.47~69 (2003)
- 차영주, "면역혈청검사 신빙도조사 결과보고(2004)", 상검사와정도관리, 27(1), pp.37~57 (2005), 대한임상검사정도관리협회
- 차영주, "면역혈청검사 신빙도조사 결과보고 (2005)", 임상검사와정도관리, 28(1), pp.41~61 (2006), 대한임상검사정도관리협회
- 차영주, 권소영, 김신규, 김재룡, 김현숙, 박명희, 박성훈, 박애자,, "Annual report on external quality assessment in immunoserology in Korea", 임상검사와정도관리, 29(1), pp.45~64 (2007), 대한임상검사정도관리협회
- College of American Pathologists, "Surveys 2005 G-A Syphilis Serology Participant Summary" (2005), CAP
- College of American Pathologists, "Surveys 2005 G-B Syphilis Serology Participant Summary" (2005), CAP
- College of American Pathologists, "Surveys 2005 G-C Syphilis Serology Participant Summary" (2005), CAP
- College of American Pathologists, "Surveys 2006 G-A Syphilis Serology Participant Summary" (2006), CAP
- College of American Pathologists, "Surveys 2006 G-B Syphilis Serology Participant Summary" (2006), CAP
- College of American Pathologists, "Surveys 2006 G-C Syphilis Serology Participant Summary" (2006), CAP
- Castro AR, "Defibrination of blood plasma for use in serological tests for syphilis", Clin Diagn Lab Immunol, 9, pp.1376~1378 (2002)
- Cole M, "Five syphilis agglutination assays,MHRA Report number 04007" (2004), Medicines and Healthcare products Regulatory Agency
- Mabey D, "Prospective, multi-centre clinic-based evaluation of four rapid diagnostic tests for syphilis", Sex Transm Infect, 82(S), pp.v13~v16 (2006)