송이줄기 첨가에 따른 Muscat Bailey A 포도의 발효 중 발효액 및 포도주의 생리활성 물질 함량
Phenolic compounds of must and wine supplemented with Muscat Bailey A grape fruit stem
0
조회
0
다운로드
10
인용
0
참고문헌
키워드
초록
이전 연구에서 포도 품종별 및 부위별 생리활성 물질을 분석한 결과 MBA 품종의 송이줄기 부위에서 trans- resveratrol 함량이 다른 품종에 비해 수십배 높았다. 이를 바탕으로 포도주 제조 시 버려지는 비가식 부위인 송이줄기를 농도별로 첨가해 발효 중 발효액 및 포도주의 폴리페놀 함량을 조사하였다. 송이줄기 첨가량에 따른 발효액의 발효 중 적색도, 총안토시안 함량, 총폴리페놀 및 탄닌 함량은 발효후기로 갈수록 송이줄기 첨가에 따라 유의적으로 증가하였고, 포도주에서도 같은 경향을 보였다. 반면 포도주의 휘발산 함량은 송이줄기 첨가량이 증가할수록 감소하는 경향을 보였다. 발효액 및 포도주의 폴리페놀 성분을 분석한 결과 catechin(8.16∼23.08 mg/L)함량이 가장 높았으며 gallic acid(2.32∼3.28 mg/L), trans-resveratrol(1.38∼3.27 mg/L)및 ferulic acid(1.51∼1.59 mg/L) 순서로 함량이 높았다. MBA 포도주의 항산화활성을 DPPH IC50로 측정한 결과 송이줄기 함량에 비례해 활성이 증가하였으며, 송이줄기 5% 첨가한 포도주(12 mg/L)는 ascorbic acid의 DPPH IC50 (67 mg/L)보다 낮은 농도에서 높은 항산화활성을 나타내었다.
Abstract
This study investigated the phenolic compounds of must and wine supplemented with different concentrations (0% (no added stems), 1%, 2%, 3%, and 5%) of fruit stems during winemaking using Muscat Bailey A (MBA) grapes. The red color, and total anthocyanin, total polyphenol, and tannin contents of the must and wine significantly (p<0.05) increased with increasing added amounts of grape fruit stems, while the volatile acid content decreased with increasing added amounts of grape fruit stems. Catechin (8.16∼23.08 mg/L), gallic acid (2.32∼3.28 mg/L), trans-resveratrol (1.38∼3.27 mg/L), and ferulic acid (1.51∼1.59 mg/L) were detected in the must and wine via HPLC. The bioactive substance contents increased with increasing added amounts of grape fruit stems, except for ferulic acid. The DPPH IC50 activity was higher in the wine (12 mg/L) with 5% grape fruit stems than in ascorbic acid (67 mg/L). These results suggest that the fruit stems of MBA grapes can be used as functional materials for winemaking.
참고문헌 (39)
- Scalbert A, "Dietary intake and bioavailability of polyphenols", American Soc Nutrition, 130, pp.2073~2085 (2000)
- Bautista-Ortín AB, "The effects of enological practices in anthocyanins, phenolic compounds and wine colour and their dependence on grape characteristics", J Food Comp Anal, 20, pp.546~552 (2007)
- Bub A, "Fruit juice consumption modulates antioxidative status, immune status, and DNA damage", J Nutr Biochem, 14, pp.90~98 (2003)
- Gomez-Cordoves MC, "Interpretation of color variables during the aging of red wines : relationship with families of phenolic compounds", J Agric Food Chem, 43, pp.557~561 (1995)
- Park YK, "Daily grape juice consumption reduces oxidative DNA damage and plasma free radicla levels in healthy Koreans", Mutat Res, 529, pp.77~86 (2003)
- Lugasi A, "Antioxidant properties of commercial alcoholic and nonalcoholic beverages", Nahr Food, 47, pp.79~86 (2003)
- Anastasiadi M, "Antilisterial activities of polyphenol rich extracts of grapes and vinification byproducts", J Agric Food Chem, 57, pp.457~463 (2009)
- Careri M, "Liquid chromatography-electrospray tandem mass spectrometry of cis-resveratrol and trans-resveratrol : development, validation, and application of the method to red wine, grape, and winemaking byproducts", J Agric Food Chem, 52, pp.6868~6874 (2004)
- Neuza P, "Quantification of polyphenols with potential antioxidant properties in wines using reverse phase HPLC", J Sep Sci, 31, pp.2189~2198 (2008)
- Goldberg DM, "A global survey of trans-resveratrol concentrations in commercial wines", American J Enol Viticult, 46, pp.159~165 (1995)
- 장은하, "국내 육성 신품종 포도의 부위별 생리활성 물질의 함량", 한국식품과학회지, 45(6), pp.708~713 (2013), 한국식품과학회[DOI]
- Koh JS, "Laboratory manual for food analysis", pp.31~33 (1998), Jeju University Press
- Mazza G, "Anthocyanins, phenolics and color of cabernet franc, merlot, and pinot noir wines from british columbia", J Agric Food Chem, 47, pp.4009~4017 (1999)
- Folin O, "On tyrosine and tryptophane determination in proteins", J Bio Chem, 27, pp.625~650 (1927)
- Blois MS, "Antioxidant determination by the use of a stable free radical", Nature, 181, pp.1199~1201 (1958)
- 장은하, "Carbonic Maceration처리에 의한 Campbell Early 발효액의 감산 효과: 사과산 대사 관련 효소활성의 영향", 한국식품저장유통학회지, 18(5), pp.795~802 (2011), 한국식품저장유통학회
- Drysdale GS, "Acetic acid bacteria in some Australian wines", Food Tech Australia, 37, pp.17~20 (1985)
- Eglinton J, "The occurrence of volatile acidity in Australian wines", Australian New Zealand Grapegrower Winemaker, 426, pp.7~12 (1999)
- Eglinton J, "Restarting incomplete fermentations : the effect of high concentrations of acetic acid", Australian J Grape Wine Res, 5, pp.71~78 (1999)
- 장은하, "포도주 품질에 미치는 알코올 발효전 포도 전처리 효과", 한국식품저장유통학회지, 15(6), pp.824~831 (2008), 한국식품저장유통학회
- Pastorkova E, "Growth inhibitory effect of grape phenolics against wine spoilage yeasts and acetic acid bacteria", Inter J Food Microbi, 161, pp.209~213 (2013)
- Rentzsch M, "Isolation and identification of 5-carboxy-pyranoanthocyanins in beverages from cherry(Prunus cerasus L. )", Innovat Food Sci Emerg Tech, 8, pp.333~338 (2007)
- Fenwick GR, "Bitter principles in food plants. In: Bitterness in Foods and Beverages, Developments in Food Science", pp.205~250 (1990), Elsevier Science Publishers
- Bautista-Ortin AB, "The effects of enological practices in anthocyanins, phenolic compounds and wine colour and their dependence on grape characteristics", J Food Comp Anal, 20, pp.546~552 (2007)
- 장은하, "국산 및 수입 적포도주의 품질특성", 한국식품저장유통학회지, 15(2), pp.203~208 (2008), 한국식품저장유통학회
- Sun B, "Effect of different winemaking technologies on phenolic composition in tinta miuda red wines", J Agric Food Chem, 49, pp.5809~5816 (2001)
- Kantz K, "Isolation and determination of polymeric polyphenols in wines using Sephadex LH-20", American J Enol Vitic, 42, pp.309~315 (1991)
- Auw JM, "Effect of processing on the phenolics and color of Cabernet Sauvignon, Chambourcin and Noble wines and juices", American J Enol Vitic, 47, pp.279~286 (1996)
- Apostolou A, "Assessment of polyphenolic content, antioxidant activity, protection against ROS-induced DNA damage and anticancer activity of Vitis vinifera stem extracts", Food Chem Toxicol, 61, pp.60~68 (2013)
- Pezet R, "Glycosylation and oxidative dimerization of resveratrol are respectively associated to sensitivity and resistance of grapevine cultivars to downy mildew", Physiol Mol Plant Pathol, 65, pp.297~303 (2004)
- Chong J, "Metabolism and roles of stilbenes in plants", Plant Sci, 177, pp.143~155 (2009)
- Fabris S, "Antioxidant properties of resveratrol and piceid on lipid peroxidation in micelles and monolamellar liposomes", Biophys Chem, 135, pp.76~83 (2008)
- Lorenz P, "Oxyresveratrol and resveratrol are potent antioxidants and free radical scavengers : effect on nitrosative and oxidative stress derived from microglial cells", Nitric Oxide, 9, pp.64~76 (2003)
- Regev-Shoshani G, "Glycosylation of resveratrol protects it from enzymic oxidation", Biochem J, 374, pp.157~163 (2003)
- Cheng YH, "The effects of ferulic acid on nucleus pulposus cells under hydrogen peroxide-induced oxidative stress", Proc Biochem, 46, pp.1670~1677 (2011)
- 문광덕, "Influence of Additions of Seeds and Stems on the Properties of Processed Campbell Grape Juice", 한국식품저장유통학회지, 14(3), pp.256~262 (2007), 한국식품저장유통학회
- 장은하, "발효전 포도 으깸이의 열처리 온도에 따른 와인 품질특성", 한국식품저장유통학회지, 17(5), pp.608~615 (2010), 한국식품저장유통학회
- Atanackovic M, "Influence of winemaking techniques on the resveratrol content, total phenolic content and antioxidant potential of red wines", Food Chem, 131, pp.513~518 (2012)
- Lacopini P, "Catechin, epicatechin, quercetin, rutin and resveratrol in red grape : content in vitro antioxidant activity and interactions", J Food Comp Anal, 21, pp.589~598 (2008)
