Aureobasidium pullulans으로 부터 분리한 β-1,3/1,6-glucan의 면역활성의 연구
An in vitro study of immune activity by β-1,3/1,6-glucan isolated from Aureobasidium pullulans
2
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
β-Glucan is a natural compound contained in cell walls of yeast or fungi, and cereal’s fiber. It is also known to boost the immune system in human. Aureobasidium is a producer of water-soluble β-1,3/1,6-glucan. In this study, natural killer (NK) cell and macrophage activity were tested to investigate the effects of β-1,3/1,6-glucan isolated from A. pullulans on immune activity. Activation of NK cell was increased about 63-39% by the treatment of 10-200 μg/mL β-1,3/1,6-glucan than control. Besides, only 10 μg/mL of β-1,3/1,6-glucan was enough to boost activation of NK cell. Phagocytosis of macrophage was increased to 15~21% by the treatment of 10~200 μg/mL of β-1,3/1,6-glucan than zymosan-treatment. In LP-BM5 proliferating inhibition test, relative mRNA level of LP-BM5 virus was decreased in β-1,3/1,6-glucan-treated cell about 36~74% than control. The decline of LP-BM5 mRNA level appeared to depend on the concentration of β-1,3/1,6-glucan. These results suggest that pure β-1,3/1,6-glucan from A. pullulans might be contributing to enhancement of immune activity through the activation of NK cell and phagocytosis of macrophage. Moreover, treatment of the β-1,3/1,6-glucan could increase the resistance to virus infection such as LP-BM5 through the restraining of the multiplication.
Abstract
β-Glucan is a natural compound contained in cell walls of yeast or fungi, and cereal’s fiber. It is also known to boost the immune system in human. Aureobasidium is a producer of water-soluble β-1,3/1,6-glucan. In this study, natural killer (NK) cell and macrophage activity were tested to investigate the effects of β-1,3/1,6-glucan isolated from A. pullulans on immune activity. Activation of NK cell was increased about 63-39% by the treatment of 10-200 μg/mL β-1,3/1,6-glucan than control. Besides, only 10 μg/mL of β-1,3/1,6-glucan was enough to boost activation of NK cell. Phagocytosis of macrophage was increased to 15~21% by the treatment of 10~200 μg/mL of β-1,3/1,6-glucan than zymosan-treatment. In LP-BM5 proliferating inhibition test, relative mRNA level of LP-BM5 virus was decreased in β-1,3/1,6-glucan-treated cell about 36~74% than control. The decline of LP-BM5 mRNA level appeared to depend on the concentration of β-1,3/1,6-glucan. These results suggest that pure β-1,3/1,6-glucan from A. pullulans might be contributing to enhancement of immune activity through the activation of NK cell and phagocytosis of macrophage. Moreover, treatment of the β-1,3/1,6-glucan could increase the resistance to virus infection such as LP-BM5 through the restraining of the multiplication.
참고문헌 (32)
- Chang R, "Functional properties of edible mushrooms", Nutrition, 54, pp.694~696 (1996)
- Cheung PCK, "The nutritional and health benefits of mushrooms", Nutrition Bull, 35, pp.292~299 (2010)
- Akramiene D, "Effects of beta-glucans on the immune system", Medicina (Kaunas), 43, pp.597~606 (2007)
- Ohno N, "Antitumor 1, 3-beta-Glucan from Cultured Fruit Body of Sparassis crispa", Biol Pharm Bull, 23, pp.866~872 (2000)
- Mori K, "Antiatherosclerotic effect of the edible mushrooms Pleurotus eryngii(Eringi), Grifola frondosa(Maitake), and Hypsizygus marmoreus(Bunashimeji)in apolipoprotein E–deficient mice", Nutr Res, 28, pp.335~342 (2008)
- Hyeong-Dong Kim, "Effects of β-Glucan from Aureobasidium pullulans on Acute Inflammation in Mice", Archives of Pharmacal Research, 30(3), pp.323~328 (2007), 대한약학회
- Muramatsu D, "β-glucan derived from Aureobasidium pullulans is effective for the prevention of influenza in mice", PLoS One, 7, pp.e41399~ (2012)
- Brennan CS, "The potential use of cereal(1→ 3, 1→ 4)-β-D-glucans as functional food ingredients", J Cereal Sci, 42, pp.1~13 (2005)
- Cahill A, "β-glucan process, additive and food product. US Patent No. 2003-01-48015", ~ (2003)
- 권혜진, "새송이버섯(Pleurotus eringii) 추출물의 미백효능 평가", 아시안뷰티화장품학술지, 13(5), pp.695~700 (2015), 한국피부과학연구원
- McIntosh M, "Curdlan and other bacterial(1→ 3)-β-D-glucans", Appl Microbiol Biotechnol, 68, pp.163~173 (2005)
- Wilson SM, "Determining the subcellular location of synthesis and assembly of the cell wall polysaccharide(1, 3;1, 4)-β-glucan in grasses", Plant Cell, 27, pp.754~771 (2015)
- Cui SW, "Cell wall polysaccharides in cereals: chemical structures and functional properties", Structural Chemistry, 20, pp.291~297 (2009)
- Matse AM, "Effects of high isostatic pressure on mushrooms", J Food Engineering, 45, pp.11~16 (2000)
- Kimura Y, "Antitumor and antimetastatic activity of a novel water-soluble low molecular weight β-1, 3-D-glucan(branch β-1, 6)isolated from Aureobasidium pullulans 1A1 strain black yeast", Anticancer Res, 26, pp.4131~4141 (2006)
- 윤현수, "Immunomodulatory Effects of Aureobasidium pullulans SM-2001 Exopolymers on Cyclophosphamide-Treated Mice", Journal of Microbiology and Biotechnology, 20(2), pp.438~445 (2010), 한국미생물·생명공학회
- Brown GD, "Immune recognition of fungal β glucans", Cell Microbiol, 7, pp.471~479 (2005)
- Brown GD, "Dectin-1 : a signalling non-TLR pattern-recognition receptor", Nat Rev Immunol, 6, pp.33~43 (2006)
- Lee, D.H., "Innate immunity induced by fungal β-glucans via dectin-1 signaling pathway", Int J Med Mushrooms, 16, pp.1~16 (2014)
- Brown GD, "Dectin-1 is a major β-glucan receptor on macrophages", J Exp Med, 196, pp.407~412 (2002)
- Taylor PR, "The β-glucan receptor, dectin-1, is predominantly expressed on the surface of cells of the monocyte", macrophage and neutrophil lineages, 2002, pp.169, 3876~3882
- Willment JA, "The human β‐glucan receptor is widely expressed and functionally equivalent to murine Dectin‐1 on primary cells", Eur J Immunol, 35, pp.1539~1547 (2005)
- Chakraverty R, "The role of antigen-presenting cells in triggering graft-versus-host disease and graft-versus-leukemia", Blood, 110, pp.9~17 (2007)
- Chan GCF, "The effects of β-glucan on human immune and cancer cells", J Hemato Oncol, 2, pp.1~11 (2009)
- Li W, "Immunotherapy of murine retrovirus-induced acquired immunodeficiency by CD4 T regulatory cell depletion and PD-1 blockade", J Virol, 85, pp.13342~13353 (2011)
- Jolicoeur P, "Murine acquired immunodeficiency syndrome (MAIDS): an animal model to study the AIDS pathogenesis", FASEB J, 5, pp.2398~2405 (1991)
- O’Connor MA, "Reciprocal relationship of T regulatory cells and monocytic myeloid derived suppressor cells in LP-BM5 murine retrovirus-induced immunodeficiency", J Gen Viro, 97, pp.509~522 (2016)
- Kim MS, "Aureobasidium pullulans ksy-0516 mutants producing extracellular secretion β-1,3", 1,6-glucan. Korea patent No. 10-2014-0078624, 2014
- Vetvicka V, "Effects of marine β−1,3 glucan on immune reactions", Int Immunopharmacol, 4, pp.721~730 (2004)
- 서이슬, "천년초에서 분리한 점질다당의 면역자극 활성", 한국식품영양과학회지, 41(1), pp.95~102 (2012), 한국식품영양과학회
- Hoffman OA, "Fungal β-glucans modulate macrophage release of tumor necrosis factor-α in response to bacterial lipopolysaccharide", Immunol Lett, 37, pp.19~25 (1993)
- Chen CY, "Effects of Ruoye polysacchrides and their derivatives on mice infected with LP BM5 MuLV, a murine AIDS model", Chinese Pharmacol Bull, 15, pp.336~339 (1999)
