기능성 식품소재 개발을 위한 야생 곰취잎 추출물의 생리활성
Biological activity of wild Ligularia fischeri leaf extracts in the development of functional food materials
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The objective of this study was to identify the anti-oxidation, astringent, and inhibition effects of wild Ligularia fischeri on hyaluronidase and angiotensin conerting enzyme (ACE). In order to identify the total phenolic compound (TPC), various solvents were used for extraction showing hot water extract with the highest value of 14.42 GAE mg/g. In addition, ABTS radical scavenging activity measurements revealed an anti-oxdiation effect of 98.64-99.84% a hot water extract concentration of 50-200 μg/mL and a radical scavenging activity of 95.14-98.96% at a 60% ethanol extract content. If expressed in antioxidant protection factors (PF), the hot water extract showed 0.59-1.02 PF and the 60% ethanol sample displayed 0.30-0.74 PF. To identify the bio-activity effect, the hyaluronidase inhibition effect was determined as 4.66-35.00% in a 50-200 μg/mL hot water extract. Considering ACE inhibition effect, the hot water extract and 60% ethanol sample showed 0-64.24% and 46.12-69.64% inhibition effect, respectively. Lastly, when taking into account the astringent effect, the hot water extract with 50-200 μg/mL TPC concentration showed 15.68-26.92% and the 60% ethanol sample with an equal concentration exhibited 49.48-86.84%, which indicates the possibility to apply this product as a cosmetic source for pore contraction. Therefore, wild Ligularia fischeri extract can be used for anti-inflammation, high-blood pressure prevention, and as a source for health functional food with anti-oxidative properties.
Abstract
The objective of this study was to identify the anti-oxidation, astringent, and inhibition effects of wild Ligularia fischeri on hyaluronidase and angiotensin conerting enzyme (ACE). In order to identify the total phenolic compound (TPC), various solvents were used for extraction showing hot water extract with the highest value of 14.42 GAE mg/g. In addition, ABTS radical scavenging activity measurements revealed an anti-oxdiation effect of 98.64-99.84% a hot water extract concentration of 50-200 μg/mL and a radical scavenging activity of 95.14-98.96% at a 60% ethanol extract content. If expressed in antioxidant protection factors (PF), the hot water extract showed 0.59-1.02 PF and the 60% ethanol sample displayed 0.30-0.74 PF. To identify the bio-activity effect, the hyaluronidase inhibition effect was determined as 4.66-35.00% in a 50-200 μg/mL hot water extract. Considering ACE inhibition effect, the hot water extract and 60% ethanol sample showed 0-64.24% and 46.12-69.64% inhibition effect, respectively. Lastly, when taking into account the astringent effect, the hot water extract with 50-200 μg/mL TPC concentration showed 15.68-26.92% and the 60% ethanol sample with an equal concentration exhibited 49.48-86.84%, which indicates the possibility to apply this product as a cosmetic source for pore contraction. Therefore, wild Ligularia fischeri extract can be used for anti-inflammation, high-blood pressure prevention, and as a source for health functional food with anti-oxidative properties.
참고문헌 (26)
- 장상근, "곰취 분말 및 당귀 열수추출물의 생리활성을 활용한 기능성 냉면의 제조", 한국식생활문화학회지, 23(4), pp.479~488 (2008), 한국식생활문화학회
- Ham SS, "Antimutagenic and antigenotoxic effects of Ligularia fischeri extracts", J Korean Soc Food Sci Nutr, 27, pp.745~750 (1998)
- 조순덕, "곰취의 식품재료화를 위한 품질특성 및 가공제품에 대한 연구", Food Science and Preservation, 12(1), pp.43~47 (2005), 한국식품저장유통학회
- Na Y, "Effect of antioxidation and inihbition of matrix metalloproteinase-1 from Ligularia fischeri", J Soc Cosmet Sci Kor, 32, pp.129~134 (2006)
- Ham SS, "Cytotoxicity of Ligularia fischeri extracts", J Korean Soc Food Sci Nutr, 27, pp.987~992 (1998)
- 권영주, "마이크로웨이브 추출조건에 따른 곰취 추출물의 총 폴리페놀 함량 및 항산화작용의 변화", Food Science and Preservation, 9(3), pp.332~337 (2002), 한국식품저장유통학회
- Gonzalez R, "Effects of flavonoids and other polyphenols on inflammation", Crit Rev Food Sci Nutr, 51, pp.331~362 (2011)[DOI]
- Folin O, "On phosphotungsticphosphomolybdic compounds as color reagents", J Biol Chem, 12, pp.239~243 (1912)
- Fellegrin N, "Screening of dietary carotenoids and carotenoid-rich fruit extracts for antioxidant activities applying 2,2'-azinobis (3-ethylenebenzothiazoline-6-sulfonic acid radical cation decolorization assay", Methods Enzymol, 299, pp.379~389 (1998)
- Andarwulan N, "Phenolic content in differentiated tissue cultures of untransformed and Agrobacterium-transformed roots of anise (Pimpinella anisum L.)", J Agric Food Chem, 47, pp.1776~1780 (1999)[DOI]
- Dorfman A, "A turbidimetric method for the assay of hyaluronidase", J Biol Chem, 172, pp.367~375 (1948)
- Cushman DW, "Spectrophotometric assay and properties of the angiotensin-converting enzyme of rabbit lung", Biochem Pharmacol, 20, pp.1637~1648 (1971)[DOI]
- 이진태, "함초의 생리활성기능과 화장품소재로서의 응용", 대한본초학회지, 17(2), pp.6~60 (2002), 대한본초학회
- Vogt T, "Phenylpropanoid biosynthesis", Molecular Plant, 3, pp.2~20 (2010)[DOI]
- Pandey KB, "Plant polyphenols as dietary antioxidants in human health and disease", Oxid Med Cell Longevity, 2, pp.270~278 (2009)[DOI]
- 한소라, "구름버섯(Coriolus versicolor)의 용매 추출물에 대한 항균 및 항산화 활성 조사", 한국식품영양과학회지, 44(12), pp.1793~1798 (2015), 한국식품영양과학회
- 송원영, "조릿대 추출물의 용매별 항산화 및 항염증 활성", 농업생명과학연구, 49(3), pp.145~154 (2015), 농업생명과학연구원[DOI]
- Toyosaki T, "Antioxidant effect of <TEX>${\beta}$<", TEX>-carotene on lipid peroxidation and synergism with tocopherol in an emulsified linoleic acid model system, 2002, pp.53, 419~423
- Jackson DG, "Immunological functions of hyaluronan and its receptors in the lymphatics", Immunol Rev, 230, pp.216~231 (2009)[DOI]
- Fronza M, "Hyaluronidase modulates inflammatory response and accelerates the cutaneous wound healing", PLoS One, 9, pp.e112297~ (2014)[DOI]
- 안뜰에봄, "In Vitro Cytotoxicity, Skin Regeneration, Anti-wrinkle, Whitening and In Vivo Skin Moisturizing Effects of Oncheongeum", 대한한방부인과학회지, 29(1), pp.14~34 (2016), 대한한방부인과학회[DOI]
- 조용복, "황칠나무 추출물이 고혈압에 미치는 영향에 관한 연구", 한국산학기술학회논문지, 17(11), pp.708~715 (2016), 한국산학기술학회[DOI]
- 강승미, "아이스플랜트(Mesembryanthemum crystallinum)의 3T3-L1 전지방세포 분화 및 Angiotensin-Converting Enzyme(ACE) 활성 억제", 한국식품영양과학회지, 46(8), pp.1012~1017 (2017), 한국식품영양과학회
- 권유리, "추출용매에 따른 모링가(Moringa oleifera Lam.) 잎의 항산화 및 생리활성 효과", Food Science and Preservation, 21(6), pp.831~837 (2014), 한국식품저장유통학회
- Lesschaeve I, "Polyphenols: factors influencing their sensory properties and their effects on food and beverage preferences", Am J Clin Nutr, 81, pp.330~335 (2005)[DOI]
- 이재은, "층꽃나무(Caryopteris incana Miq.) 추출물의 생리활성", Journal of Applied Biological Chemistry, 60(1), pp.61~68 (2017), 한국응용생명화학회