본문 바로가기
KERD
논문KCI 등재

Physicochemical properties and immunomodulatory effects of polysaccharides isolated from Styela plicata

2

조회

0

다운로드

0

인용

0

참고문헌

키워드

주제분류식품과학

초록

The physicochemical properties and immunomodulatory effects of polysaccharides isolated from the sea squirt Styela plicata were investigated. The crude polysaccharide (SP) was further fractionated into an unabsorbed fraction (SF-1) and absorbed fractions (SF-2 and SF3) to isolate polysaccharides with different molecular weights. The major constituents in the crude and fractionated polysaccharides were total carbohydrate (28.03-42.65%), uronic acid (9.88-23.76%), sulfate (18.03-29.25%), and protein (1.48-26.47%). The average molecular weights of the fractionated polysaccharides ranged from 28 to 1,187 kDa. The significant monosaccharide components of the polysaccharides were galactose (27.91-62.21%), glucose (14.79-37.63%), and glucosamine (10.74-28.31%). FT-IR analysis indicates that SP, SF-1, SF-2, and SF-3 were sulfated polysaccharides. SF-1 increased the production of nitric oxide and cytokines, such as tumor necrosis factor alpha and interleukin 6, in a dose-dependent manner in RAW 264.7 cells. Further, polymerase chain reaction analysis revealed that the mRNA expression of inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2) was markedly increased. These results indicate that SF-1 can enhance the activation of macrophages and that increasing the molecular weight and the uronic acid and sulfate content of fractionated polysaccharides can enhance their immunomodulatory function.

Abstract

The physicochemical properties and immunomodulatory effects of polysaccharides isolated from the sea squirt Styela plicata were investigated. The crude polysaccharide (SP) was further fractionated into an unabsorbed fraction (SF-1) and absorbed fractions (SF-2 and SF3) to isolate polysaccharides with different molecular weights. The major constituents in the crude and fractionated polysaccharides were total carbohydrate (28.03-42.65%), uronic acid (9.88-23.76%), sulfate (18.03-29.25%), and protein (1.48-26.47%). The average molecular weights of the fractionated polysaccharides ranged from 28 to 1,187 kDa. The significant monosaccharide components of the polysaccharides were galactose (27.91-62.21%), glucose (14.79-37.63%), and glucosamine (10.74-28.31%). FT-IR analysis indicates that SP, SF-1, SF-2, and SF-3 were sulfated polysaccharides. SF-1 increased the production of nitric oxide and cytokines, such as tumor necrosis factor alpha and interleukin 6, in a dose-dependent manner in RAW 264.7 cells. Further, polymerase chain reaction analysis revealed that the mRNA expression of inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2) was markedly increased. These results indicate that SF-1 can enhance the activation of macrophages and that increasing the molecular weight and the uronic acid and sulfate content of fractionated polysaccharides can enhance their immunomodulatory function.

참고문헌 (40)

  1. Hooper LV, "Interactions between the microbiota and the immune system", Science, 336, pp.1268~1273 (2012)
  2. Klimp AH, "A potential role of macrophage activation in the treatment of cancer", Crit Rev Oncol Hematol, 44, pp.143~161 (2002)
  3. Commins SP, "Immunologic messenger molecules: cytokines, interferons, and chemokines", J Allergy Clin Immunol, 125, pp.53~72 (2010)
  4. Medzhitov R, "Innate immune recognition : mechanisms and pathways", Immunol Rev, 173, pp.89~97 (2000)
  5. Lee JS, "Purification, characterization and immunomodulating activity of a pectic polysaccharide isolated from Korean mulberry fruit Oddi (Morus alba L.)", Int Immunopharmacol, 17, pp.858~866 (2013)
  6. Li JE, "Isolation and partial characterization of a neutral polysaccharide from Mosla chinensis Maxim. cv. Jiangxiangru and its antioxidant and immunomodulatory activities", J Funct Foods, 6, pp.410~418 (2014)
  7. Schepetkin IA, "Botanical polysaccharides : macrophage immunomodulation and therapeutic potential", Int Immunopharmacol, 6, pp.317~333 (2006)
  8. Liu J, "A review of bioactive plant polysaccharides: Biological activities, functionalization, and biomedical applications", Bioact Carbohydr Diet Fibre, 5, pp.31~61 (2015)
  9. Yang W, "Purification and structural characterization of Chinese yam polysaccharide and its activities", Carbohydr Polym, 117, pp.1021~1027 (2015)
  10. Ko SC, "Effect of angiotensin I-converting enzyme(ACE)inhibitory peptide purified from enzymatic hydrolysates of Styela plicata", Eur Food Res Technol, 233, pp.915~922 (2011)
  11. Yang Y, "In vitro antioxidant activities of sulfated polysaccharide fractions extracted from Corallina officinalis", Int J Biol Macromol, 49, pp.1031~1037 (2011)
  12. Gan D, "Production, preliminary characterization and antitumor activity in vitro of polysaccharides from the mycelium of Pholiota dinghuensis Bi", Carbohydr Polym, 84, pp.997~1003 (2011)
  13. Sun Z, "Purification, characterization and immunomodulatory activity of a polysaccharide from Celosia cristata", Carbohydr Polym, 133, pp.337~344 (2015)
  14. David GK, "Latitudinal variability in spatial genetic structure in the invasive ascidian, Styela plicata", Mar Biol, 157, pp.1955~1965 (2010)
  15. Maltagliati F, "The genetic structure of the exotic ascidian Styela plicata (Tunicata) from Italian ports, with a re-appraisal of its worldwide genetic pattern", Mar Ecol, 37, pp.492~502 (2016)
  16. 서보영, "오만둥이(Styela plicata)의 항유전독성 및 대장암 억제효과에 관한 연구", 한국식품영양과학회지, 38(7), pp.839~845 (2009), 한국식품영양과학회
  17. Nair SV, "A C-type lectin from the tunicate, Styela plicata, that modulates cellular activity", Comp Biochem Part C: Toxicol Pharmacol, 129, pp.11~24 (2001)
  18. Dubois M, "Colorimetric method for determination of sugars and related substances", Anal Chem, 28, pp.350~356 (1956)
  19. Cesaretti M, "A 96-well assay for uronic acid carbazole reaction", Carbohydr Polym, 54, pp.59~61 (2003)
  20. Dodgson KS, "A note on the determination of the ester sulphate content of sulphated polysaccharides", Biochem J, 84, pp.106~110 (1962)
  21. Lowry OH, "Protein measurement with the Folin phenol reagent", J Biol Chem, 193, pp.265~275 (1951)
  22. Schepetkin IA, "Botanical polysaccharides : macrophage immunomodulation and therapeutic potential", Int Immunopharmacol, 6, pp.317~333 (2006)
  23. Leiro JM, "Immunomodulating activities of acidic sulphated polysaccharides obtained from the seaweed Ulva rigida C. Agardh", Int Immunopharmacol, 7, pp.879~888 (2007)
  24. Zhang Q, "Antioxidant activities of sulfated polysaccharide fractions from Porphyra haitanesis", J Appl phycol, 15, pp.305~310 (2003)
  25. Zhou G, "In vivo antitumor and immunomodulation activities of different molecular weight lambda-carrageenans from Chondrus ocellatus", Pharmacol Res, 50, pp.47~53 (2004)
  26. Pavao MS, "Structural heterogeneity among unique sulfated L-galactans from different species of ascidians(tunicates)", J Biol Chem, 264, pp.9972~9979 (1989)
  27. Mourao PA, "Structural features of sulfated glycans from the tunic of Styela plicata(Chordata Tunicata)", Eur J Biochem, 166, pp.431~436 (1987)
  28. Albano RM, "Isolation, fractionation, and preliminary characterization of a novel class of sulfated glycans from the tunic of Styela plicata (Chordata Tunicata)", J Biol Chem, 261, pp.758~765 (1986)
  29. Yang L, "Chemical structural and chain conformational characterization of some bioactive polysaccharides isolated from natural sources", Carbohydr Polym, 76, pp.349~361 (2009)
  30. Jiang C, "Extraction, preliminary characterization, antioxidant and anticancer activities in vitro of polysaccharides from Cyclina sinensis", Carbohydr Polym, 84, pp.851~857 (2011)
  31. Qiao D, "Extraction optimized by using response surface methodology, purification and preliminary characterization of polysaccharides from Hyriopsis cumingii", Carbohydr Polym, 76, pp.422~429 (2009)
  32. Wang ML, "Immunomodulatory activities of Gelidium amansii gel extracts on murine RAW 264. 7 macrophages", J Food Drug Anal, 21, pp.397~403 (2013)
  33. Yu Q, "Macrophage immunomodulatory activity of a purified polysaccharide isolated from Ganoderma atrum", Phytother Res, 27, pp.186~191 (2013)
  34. Kadowaki T, "Induction of nitric oxide production in RAW264. 7 cells under serum-free conditions by O-antigen polysaccharide of lipopolysaccharide", Anticancer Res, 33, pp.2875~2879 (2013)
  35. Ruotsalainen M, "Cytotoxicity, production of reactive oxygen species and cytokines induced by different strains of Stachybotrys sp. from moldy buildings in RAW264. 7 macrophages", Environ Toxicol Pharmacol, 6, pp.193~199 (1998)
  36. Leiro JM, "Immunomodulating activities of acidic sulphated polysaccharides obtained from the seaweed Ulva rigida C. Agardh", Int Immunopharmacol, 7, pp.879~888 (2007)
  37. Schepetkin IA, "Macrophage immunomodulatory activity of polysaccharides isolated from Opuntia polyacantha", Int Immunopharmacol, 8, pp.1455~1466 (2008)
  38. Wang W, "Immunomodulatory effects of a polysaccharide purified from Lepidium meyenii Walp. on macrophages", Process Biochem, 51, pp.542~553 (2016)
  39. Na YS, "Purification, characterization and immunostimulating activity of water-soluble polysaccharide isolated from Capsosiphon fulvescens", Int Immunopharmacol, 10, pp.364~370 (2010)
  40. Karnjanapratum S, "Characterization and immunomodulatory activities of sulfated polysaccharides from Capsosiphon fulvescens", Int J Biol Macromol, 51, pp.720~729 (2012)

관련 논문AI추천

Immune-enhancing effects of polysaccharides with different molecular weights obtained from Gloiopeltis furcata이대훈, 홍주헌한국식품저장유통학회 · Food Science and Preservation2018 · 제25권 제1호Anti-inflammatory activity of a sulfated polysaccharide isolated from an enzymatic digest of brown seaweed Sargassum horneri in RAW 264.7 cells김은아, 안긴내, 전유진, 지영흔, Ilekkuttige Priyan Shanura Fernando, KALU KAPUGE ASANKA SANJEEWA한국영양학회 · Nutrition Research and Practice2017 · 제11권 제1호Immunomodulatory effects of fucoidan extracted from Sargassum fusiforme in RAW 264.7 macrophagesBomi Ryu, Jun-Geon Je, Seonghui Kim, Seonmi Ji, Seon Yeong Ji, Serim Park, Suengmok Cho, You-Jin Jeon, Yung Hyun Choi, Yunjin Choi한국수산과학회 · Fisheries and Aquatic Sciences2024 · 제27권 제12호효소종류에 따른 불등풀가사리 유래 다당류의 이화학적 특성 및 생리활성이대훈, 홍주헌한국식품저장유통학회 · Food Science and Preservation2017 · 제24권 제3호Anti-inflammatory effect of enzymatic hydrolysates from Styela clava flesh tissue in lipopolysaccharide-stimulated RAW 264.7 macrophages and in vivo zebrafish model고석천, 전유진한국영양학회 · Nutrition Research and Practice2015 · 제9권 제3호Immune Enhancement Effects of Neutral Lipids, Glycolipids, Phospholipids from Halocynthia aurantium Tunic on RAW264.7 MacrophagesJang A-yeong, Park Woo Jung, Rod-in Weerawan, Shin Il-shik한국미생물·생명공학회 · Journal of Microbiology and Biotechnology2024 · 제34권 제2호

이 간행물의 다른 논문 (9건)