약용식품 추출에 대한 항미생물 활성 검색과 폴리페놀 함량
Antimicrobial Effect of Methanol Extracts from Some Medicinal Herbs and the Content of Phenolic Compounds
2
조회
0
다운로드
54
인용
0
참고문헌
초록
다양한 기능성을 갖는 생약재 32종을 대상으로 천연보존료 개발의 일환으로 메탄올로 추출한 항균활성 물질을 몇종의 병원균과 식중독균, 식품과 관련이 있는 세균, 젖산균 및 효모 등 12종의 균주 B. subtilis, M. luteus, Sta. aureus, Sta. epidermidis, Strep. mutans, E. coli, Pse. aeruginosa, Sal. typhimurium, V. vulnificus, Lac. brevis, Leu. mesenteroides, Sacch. cerevisiae에 대하여 항균활성을 검색하였으며, 항균력이 강한 생약재 추출물은 최소저해농도(MIC)를 측정하였다. 검색한 결과 계피, 목단, 적양, 정향, 팔각향 등의 메탄올 추출물은 12균주 중 세균에 대하여 항균활성이 강하였으나, 젖산균 및 효모에 대해서는 낮은 항균활성을 보였다. 최소저해농도는 B. subtilis에 대해 강한 증식억제효과를 보였고, Sta. epidermidis나 Pse. aeruginosa에 대해서도 거의 모든 추출물이 저해활성을 나타내었다. 하지만 항균활성 측정시 거의 저해가 없었던 Lue. mesenteroides도 대부분의 추출물에서 억제 효과를 나타내 항균성과는 약간 다른 결과를 보였다. 생약재 중 강한 항균력을 보인 녹차, 정향, 적양, 인진호 등은 폴리페놀 함량이 약 7 mg/g 이상으로 높아 이들 페놀성 물질들이 항균활성에 많은 영향을 준 것으로 사료된다. 따라서 여러 미생물에 의해 변패될 수 있는 식품에서 천연보존료로의 이용이 가능할 것으로 생각된다.
Abstract
Methanol extracts were prepared from 32 medicinal herbs of the extracts were tested their microbial inhibition activities against food borne pathogens and/or food poisoning microorganisms, food-related bacteria and yeast. Methanol extracts of Cinnamomum cassia, Paeonia suffruticosa, Alnus japonica, Eugenia caryophyllata and Illicium verum exhibited antimicrobial activity for the microorganisms tested, except lactic acid bacteria and yeast. Minimum inhibitory concentrations(MIC) were about 5 mg/mL for Bacillus subtilis, Staphylococcus epidermidis and Pseudomonas aeruginosa. Cell growth of lactic acid bacteria was inhibitied, but greatly on Leuconostoc mesenteroides. The phenolic compound contents were 10.98 mg/g, 10.31 mg/g, 8.55 mg/g and 6.69 mg/g in Thea sinensis, Eugenia caryophyllata, Alnus japonica and Artenisia capillaris, respectively. Antimicrobial activity appeared to be related to phenol compound content in medicinal herbs. The methanol extracts of medicinal herbs could be suitable for the development of a food preservative.
참고문헌 (31)
- "Antibacterial substances in food. Jpn. J. Food Microbial.", pp.209~213 (1996)
- "Antimicrobial of natural medicinal herbs on the food microorganisms. Kor. J. Food Sci. Technol.", pp.957~963 (1998)
- "Antimicrobial activity of benzylisoquinoline alkaloids. Pharmazie", pp.248~250 (1987)
- "Composition and antimicrobial activity of the essential oils of Micromeria cristata subsp. phrygia and enantiomeric distribution of borneol. J. Agric. Food Chem.", pp.4300~4303 (2001)
- "Growth inhibition of Listeria monocytogenes by pure compound isolated from extract of Morus alba Linne bark. Kor. J. Food Sci. Technol.", pp.1236~1240 (1997)
- "Screening of natural antimicrobial plant extract on food spoilage microorganisms. Korean J. Food Sci. Technol."
- "The effect of hot water extract and flavour compounds of mugwort on microbial growth. J. Kor. Soc. Food Nutr.", pp.994~998 (1994)
- "Inhibitory effect of anethole and eugenol on the growth and toxin production of Aspergillus parasiticus. Intl. J. Food Microbiol."
- "Antibacterial substances in Japanese green tea extract against Streptococcus mutans", pp.2307~2312 (1989)
- "Relation between the RM value and biological activity of transhinones. J. Nanjing Coll. Pharm.", pp.42~48 (1982)
- "The antimicrobial activity of phenolic compounds against Listeria monocytogenes and their effectiveness in a model milk system. J. Food Prot. 52", rico-mu(z), pp.151~155 (1989)
- "Studies on the mechanism of the antimicrobial action of oleuropein. J. Appl. Bacteriol.", pp.559~564 (1972)
- "Effect of ethanol extracts of leaf mustard on the growth of microorganisms. J. Kor. Soc. Food Nutr.", pp.1014~1019 (1994)
- "Antimicrobial activity of water extract of green tea against cooked rice putrefactive microorganisms. Kor. J. Food Sci. Technol.", pp.66~71 (1996)
- "Antimicrobial substance in leek", pp.604~608 (1996)
- "Isolation and characterization of cinnamic acid with antimicrobial activity from needle of Pinus densiflora. Kor. J. Food Sci. Technol.", pp.823~826 (1997)
- "Naturally occurring antimicrobials in foods. Regul. Toxicol. Pharmacol.", pp.71~72 (1998)
- "Specific inhibition of Photobacterium phosphoreum extends the shelf life of modifide - atmosphere - packed cod fillets. J. Food Prot.", garcia-mu(z), pp.1191~1194 (1998)
- "Antioxidant activity of methanolic extracts of various oilseed protein ingredients. J. Food Sci.", pp.75~77 (1981)
- "A study on efficacy of Ulmi cortex. J. Kor. Soc. Pharm.", pp.217~222 (1990)
- "Screening of antimicrobial activity for medicinal herb extracts. J. Kor. Soc. Food Sci. Nutr.", pp.91~96 (1992)
- "Antimicrobial activity of Ulmi cortex extracts. J. Kor. Soc. Food Sci. Nutr.", pp.1022~1028 (1999)
- "Antioxidative and antimicrobial effects of water extracts with Castanea crenata leaf tea. Kor. J. Food Sci. Tech.", pp.1128~1131 (1999)
- "Antibacterial activities of extracts from chrysanthemun boreale M. J. Agri. Chem. Biotech. 38", pp.82~91 (1997)
- "Isolation and identification of antimicrobial compound from Sancho. Kor. J. Soc. Food Sci.", pp.56~63 (1997)
- "Antimicrobial effect of some plant extracts and their fractionates for food spoilage microorganisms. Kor. J. Food Sci. Tech.", pp.205~211 (1991)
- "Isolation and identification of antimicrobial compound from Mokdan bark", pp.1059~1065 (2003)
- "a class of semiessential food components their role in human nutrition. World Rev. Nutr. Diet.", the flavo(ids), pp.117~120 (1976)
- "Antimicrobial activity and composition of extract from Smilax china root. J. Kor. Soc. Food Nutr.", pp.574~584 (1998)
- "Isolation and identification of antimicrobial compound from sancho", pp.56~63 (1997)
- "Antimicrobial activity of phenolic constituents of Magnolia grandiflora L. J. Pharm. Sci.", pp.951~952 (1981)