들깨박으로부터 항산화 가수분해물 생산을 위한 단백질 추출 및 trypsin 가수분해 조건
Determination of protein extraction and trypsin hydrolysis conditions for producing hydrolysates with antioxidant activity from perilla seed meal
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Perilla seed meal (PSM) is an agricultural by-product that contains a lot of functional substances and protein, and is thus a functional food material. This study aimed to determine the optimal conditions of protein extraction and enzymatic hydrolysis required to produce hydrolysate with antioxidant activity from PSM. We determined the optimal conditions required to extract proteins from PSM by measuring the browning degree and protein content of extracts obtained at pH 5 and of precipitates at pH 3. The protein yield and content were high (67.3% and 109.2 mg/g, respectively), and the browning degree was low (1.00) after extraction at pH 10 and precipitation at pH 4. We hydrolyzed PSM protein with trypsin at various concentrations for different periods to establish the optimal hydrolysis conditions needed to produce PSM protein hydrolysates with antioxidant activity. The antioxidant activity was maximal in hydrolysates obtained by incubation for 3 h with 25 units of trypsin. Therefore, the optimal conditions required to extract protein from PSM comprised extraction at pH 10, precipitation at pH 4, and enzymatic hydrolysis with 25 units of trypsin for 3 h.
Abstract
Perilla seed meal (PSM) is an agricultural by-product that contains a lot of functional substances and protein, and is thus a functional food material. This study aimed to determine the optimal conditions of protein extraction and enzymatic hydrolysis required to produce hydrolysate with antioxidant activity from PSM. We determined the optimal conditions required to extract proteins from PSM by measuring the browning degree and protein content of extracts obtained at pH 5 and of precipitates at pH 3. The protein yield and content were high (67.3% and 109.2 mg/g, respectively), and the browning degree was low (1.00) after extraction at pH 10 and precipitation at pH 4. We hydrolyzed PSM protein with trypsin at various concentrations for different periods to establish the optimal hydrolysis conditions needed to produce PSM protein hydrolysates with antioxidant activity. The antioxidant activity was maximal in hydrolysates obtained by incubation for 3 h with 25 units of trypsin. Therefore, the optimal conditions required to extract protein from PSM comprised extraction at pH 10, precipitation at pH 4, and enzymatic hydrolysis with 25 units of trypsin for 3 h.
참고문헌 (26)
- Ajandouz EH, "Effect of pH on carmelization and Maillard reaction kinetics in fructose-lysine model system", J Food Sci, 66, pp.926~931 (2001)
- Bae SH, "Influences of extraction pH on the functionality of soybean protein isolate", Korean J Food Sci Technol, 30, pp.557~561 (1998)
- 백다현, "삼치 어육 가수분해물의 항산화 및 ACE 저해 활성", 한국식품영양과학회지, 48(1), pp.56~63 (2019), 한국식품영양과학회
- Chen N, "Purification and identification of antioxidant peptide from walnut (Juglans regia L.) protein hydrolysates", Peptides, 38, pp.344~349 (2012)
- Gang HJ, "Bioactive of perilla", Food Industry and Nutrition, 3, pp.65~72 (1998)
- Hoyle NT, "Quality of fish protein hydrolysates from herring(Clupea harengus)", J Food Sci, 59, pp.76~79 (1994)
- 장혜림, "알칼리에 의한 천마 단백질 추출의 최적 pH 조건", Food Science and Preservation, 22(2), pp.256~260 (2015), 한국식품저장유통학회
- 장혜림, "도루묵 가수분해물 유래 항염증 펩타이드 제조를 위한효소 가수분해 최적 조건", 한국식품과학회지, 50(2), pp.203~208 (2018), 한국식품과학회[DOI]
- 장종태, "홍게 가공부산물의 효소적 단백질 가수분해 최적화", 한국식품과학회지, 41(6), pp.622~627 (2009), 한국식품과학회
- Kim JM, "Purification and identification of an antioxidant peptide from perilla seed(Perilla frutescens)meal protein hydrolysate", Food Sci Nutr, 7, pp.1645~1655 (2019)
- 김원, "알칼리 추출법에 의한 탈지 미강 단백질 추출의 최적 pH 조건", 산업식품공학, 15(2), pp.143~147 (2011), 한국산업식품공학회
- 권효정, "Protease 처리가 누에번데기 단백질 추출 및기능성에 미치는 영향", Applied Biological Chemistry, 49(4), pp.304~308 (2006), 한국응용생명화학회
- Lee SH, "Optimal conditions for the enzymatic hydrolysis of isolated sesame meal protein", J Korean Soc Appl Biol Chem, 38, pp.248~253 (1995)
- Longvah T, "Effect of dehulling, cooking and roasting on the protein quality of Perilla frutescens seed", Food Chem, 63, pp.519~523 (1998)
- 노경희, "효소적 가수분해에 의한 홍게껍질 단백질의 특성", Journal of Nutrition and Health, 45(5), pp.429~436 (2012), 한국영양학회[DOI]
- 박보연, "들깨박단백질로부터 가수분해물과 한외여과 펩타이드 생산을 위한 효소가수분해 조건 및 항산화 활성", Food Science and Preservation, 25(5), pp.605~612 (2018), 한국식품저장유통학회
- 박주영, "국내 및 미얀마에서 재배된 삼채뿌리의 항산화, α-Glucosidase 저해 및 Acetylcholinesterase 저해 활성", Food Science and Preservation, 23(2), pp.239~245 (2016), 한국식품저장유통학회
- Rahma EH, "Effect of acetylation and succinylation of cottonseed flour on its functional properties", J Agric Food Chem, 31, pp.352~355 (1983)
- 서민철, "알칼리 법으로 추출한 갈색거저리 유충 단백질의 항염증 효능", 생명과학회지, 29(8), pp.854~860 (2019), 한국생명과학회[DOI]
- Smith PK, "Measurement of protein using bicinchoninic acid", Anal Biochem, 150, pp.76~85 (1985)
- Song PS, "Kinetic behavior and mechanism of inhibition in the Maillard reaction. Ⅰ. Kinetic behavior of the reaction between D-glucose and glycine", J Food Sci, 31, pp.906~913 (1966)
- Wang M, "Preparation and functional properties of rice bran protein isolate", J Agric Food Chem, 47, pp.411~416 (1999)
- Yamamoto H, "Antimicrobial activity of perilla seed polyphenols against oral pathogenic bacteria", Biosci Biotechnol Biochem, 66, pp.921~924 (2002)
- Zhang B, "Alkaline extraction method of cottonseed protein isolate", Mod Appl Sci, 3, pp.77~82 (2009)
- Zhang T, "Purification and characterisation of a new antioxidant peptide from chickpea (Cicer arietium L.) protein hydrolysates", Food Chem, 128, pp.28~33 (2011)
- Jianfei Zhu, "Optimization of Ultrasound-assisted Extraction Process of Perilla Seed Meal Proteins", Food Science and Biotechnology, 21(6), pp.1701~1706 (2012), 한국식품과학회[DOI]