본문 바로가기
KERD
논문KCI 등재

Inulin 분해효모 Wickerhamomyces anomalus JS01과 Saccharomyces cerevisiae KMBL7001의 혼합발효에 의한 돼지감자(Helianthus tuberosus L.)를 첨가한 약주의 발효 특성

Fermentation characteristics of yakju supplemented with Jerusalem artichoke (Helianthus tuberosus L.) and co-fermented by inulin-degrading yeast Wickerhamomyces anomalus JS01 and Saccharomyces cerevisiae KMBL7001

0

조회

0

다운로드

2

인용

0

참고문헌

키워드

주제분류식품과학

초록

Jerusalem artichoke (JA, Helianthus tuberosus L.) is a promising functional food material. JA consists of inulin and fructo-oligosaccharide which are indigestible carbohydrates in human. Previous research has develop various food products supplemented with JA, however no studies have involved yakju (Korean traditional rice wine) using JA because Saccharomyces cerevisiae cannot utilize inulin. We isolated several inulin-degrading non-Saccharomyces yeasts using nuruk (a traditional Korean fermentation starter). The isolate with the highest inulinase activity was Wickerhamomyces anomalus JS01 with 538.14 Unit/mL after 72 hours of incubation. To identify whether the inulin-degrading non-Saccharomyces yeast could improve yakju quality, W. anomalus JS01 and S. cerevisiae KMBL7001 were co-fermented to produce yakju supplemented with JA. We investigated the fermentation characteristics. At the completion of the fermentation process, the yakju co-fermented with W. anomalus JS01 and S. cerevisiae KMBL7001 (co- fermented yakju) had similar fermentation characteristics including pH, reducing sugar, alcohol, and organic acid contents compared to yakju fermented with S. cerevisiae KMBL7001 (control yakju). Moreover, co-fermented yakju had higher total amino acid contents compared with the control yakju. The W. anomalus JS01 digested some inulin into fructose which was fermented. Conversely, the control wine did not ferment any inulin during fermentation. As a result of the sensory evaluation, the co-fermented yakju obtained higher scores in all the sensory categories, suggesting that supplementing with JA and utilizing inulin-degrading yeast could improve yakju quality.

Abstract

Jerusalem artichoke (JA, Helianthus tuberosus L.) is a promising functional food material. JA consists of inulin and fructo-oligosaccharide which are indigestible carbohydrates in human. Previous research has develop various food products supplemented with JA, however no studies have involved yakju (Korean traditional rice wine) using JA because Saccharomyces cerevisiae cannot utilize inulin. We isolated several inulin-degrading non-Saccharomyces yeasts using nuruk (a traditional Korean fermentation starter). The isolate with the highest inulinase activity was Wickerhamomyces anomalus JS01 with 538.14 Unit/mL after 72 hours of incubation. To identify whether the inulin-degrading non-Saccharomyces yeast could improve yakju quality, W. anomalus JS01 and S. cerevisiae KMBL7001 were co-fermented to produce yakju supplemented with JA. We investigated the fermentation characteristics. At the completion of the fermentation process, the yakju co-fermented with W. anomalus JS01 and S. cerevisiae KMBL7001 (co- fermented yakju) had similar fermentation characteristics including pH, reducing sugar, alcohol, and organic acid contents compared to yakju fermented with S. cerevisiae KMBL7001 (control yakju). Moreover, co-fermented yakju had higher total amino acid contents compared with the control yakju. The W. anomalus JS01 digested some inulin into fructose which was fermented. Conversely, the control wine did not ferment any inulin during fermentation. As a result of the sensory evaluation, the co-fermented yakju obtained higher scores in all the sensory categories, suggesting that supplementing with JA and utilizing inulin-degrading yeast could improve yakju quality.

참고문헌 (44)

  1. Ahmed H, "Principles and Reactions of Protein Extraction, Purification, and Characterization", pp.350~352 (2004), CRC Press
  2. Byun SM, "Production of fructose from Jerusalem artichoke by enzymatic hydrolysis", J Food Sci, 43, pp.1871~1873 (1978)
  3. Chen L, "Effects of simultaneous and sequential cofermentation of Wickerhamomyces anomalus and Saccharomyces cerevisiae on physicochemical and flavor properties of rice wine", Food Sci Nutr, 9, pp.71~86 (2021)
  4. 조순덕, "포도의 관능적 품질과 객관적 품질인자 상호간의 상관성", 원예과학기술지, 28(4), pp.691~695 (2010), 한국원예학회
  5. 최정실, "시판 효모의 종류를 달리한 약주의 품질특성", 한국식품저장유통학회지, 25(6), pp.715~721 (2018), 한국식품저장유통학회
  6. Ciani M, "Non-conventional yeast species for lowering ethanol content of wines", Front Microbiol, 7, pp.642~ (2016)
  7. Costa GT, "Fructo-oligosaccharide effects on serum cholesterol levels An overview", Acta Cir Bras, 30, pp.366~370 (2015)
  8. Cunningham M, "Shaping the future of probiotics and prebiotics", Trends Microbiol, 29, pp.667~685 (2021)
  9. Duvnjak Z, "Production of sorbitol and ethanol from Jerusalem artichokes by Saccharomyces cerevisiae ATCC 36859", Appl Microbiol Biotechnol, 35, pp.711~715 (1991)
  10. Gonzalez R, "Yeast respiration of sugars by non-Saccharomyces yeast species : A promising and barely explored approach to lowering alcohol content of wines", Trends Food Sci Technol, 29, pp.55~61 (2013)
  11. 황은경, "증숙 건조 방식으로 제조한 돼지감자차의 성분 및 기능성", 한국응용과학기술학회지, 36(1), pp.1~12 (2019), 한국응용과학기술학회
  12. 황수정, "자색 돼지감자 첨가 설기떡의 항산화 활성 및 품질특성", 한국외식산업학회지, 17(3), pp.247~259 (2021), 한국외식산업학회[DOI]
  13. Ibrahim OO, "Functional oligo-saccharides : Chemicals structure, manufacturing, health benefits, applications and regulations", J Food Chem Nanotechnol, 4, pp.65~76 (2018)
  14. 정복미, "돼지감자의 품종별 유기산, 유리당 및 휘발성 향기성분", 한국식품영양과학회지, 46(7), pp.822~832 (2017), 한국식품영양과학회
  15. Kalyani Nair K, "Inulin dietary fiber with functional and health attributes : A review", Food Rev Int, 26, pp.189~203 (2010)
  16. 강지은, "누룩추출물로 제조한 약주의 품질특성", 한국식품과학회지, 48(3), pp.223~230 (2016), 한국식품과학회[DOI]
  17. 강지은, "누룩별 약주의 품질특성", 한국지역사회생활과학회지, 31(1), pp.15~24 (2020), 한국지역사회생활과학회
  18. Kango N, "Production and properties of microbial inulinases : Recent advances", Food Biotechnol, 25, pp.165~212 (2011)
  19. Kim DM, "Continuous production of gluconic acid and sorbitol from Jerusalem artichoke and glucose using an oxidoreductase of Zymomonas mobilis and inulinase", Biotechnol Bioeng, 39, pp.336~342 (1992)
  20. 김미혜, "돼지감자 분말 첨가 묵의 생리활성성분 분석 및 품질평가", 한국식품영양학회지, 28(4), pp.635~642 (2015), 한국식품영양학회
  21. 김순희, "누룩에서 분리한 국산 효모 종균(SC Y204와 Y283)으로 빚은 찹쌀막걸리의 품질 특성", 한국식품저장유통학회지, 25(7), pp.874~884 (2018), 한국식품저장유통학회
  22. Kumar CG, "Role of Materials Science in Food Bioengineering", pp.451~503 (2018), Academic Press
  23. Kurtzman CP, "A Taxonomic Study", pp.89~94 (1998), Elsevier Science Publishers
  24. Lee SB, "Effect of inoculation strategy of non-Saccharomyces yeasts on fermentation characteristics and volatile higher alcohols and esters in campbell early wines", Aust J Grape Wine Res, 25, pp.384~395 (2019)
  25. 이연리, "볶음 처리한 우엉과 돼지감자의 영양성분 및 항산화 활성", 한국식품영양학회지, 29(6), pp.870~877 (2016), 한국식품영양학회
  26. Liu GL, "Molecular characterization and expression of microbial inulinase genes", Crit Rev Microbiol, 39, pp.152~165 (2013)
  27. 나예림, "밀기울의 유산균 발효: 향기성분 및 유리아미노산 변화를 통한 제빵 소재로서의 가능성", 한국식품과학회지, 52(5), pp.524~528 (2020), 한국식품과학회
  28. Analysis Regulations of Alcoholic Beverages, "National Tax Service Liquors License Support Center", NTS, ~ (2014)
  29. Oh HI, "Food Chemistry", pp.191~192 (2008), Soohaksa
  30. 박병배, "돼지감자 분말 첨가 발효유의 이화학적 특성", Journal of Dairy Science and Biotechnology, 37(3), pp.196~205 (2019), 한국낙농식품응용생물학회[DOI]
  31. 박화연, "돼지감자 분말 첨가 쿠키의 품질특성 및 혈당에 미치는 영향", 한국지역사회생활과학회지, 24(2), pp.233~241 (2013), 한국지역사회생활과학회
  32. Park JB, "Development of Korean indigenous lowtemperature-tolerant yeast for enhancing the flavor of yakju", Fermentation, 7, pp.260~ (2021)
  33. Ravasio D, "Adding flavor to beverages with non-conventional yeasts", Fermentation, 4, pp.15~ (2018)
  34. 신동선, "콩 품종에 따른 발효물의 아미노산과 향기성분 특성", 한국식품영양학회지, 32(5), pp.434~441 (2019), 한국식품영양학회[DOI]
  35. Shin HY, "Manufacturing of non-Saccharomyces yeast starter with excellent flavor-enhancing effect of yakju by air-blast drying and characteristics of co-fermentation with S. cerevisiae", MS, pp.2021
  36. Shin SH, "Extraction and analysis of inulin from Jerusalem artichoke", Food Sci Ind, 45, pp.50~58 (2012)
  37. Slimestad R, "Norwegian-grown Jerusalem artichoke (Helianthus tuberosus L.): Morphology and content of sugars and fructo-oligosaccharides in stems and tubers", J Sci Food Agric, 90, pp.956~964 (2010)
  38. 송재철, "2단담금에서 무증자 발아현미를 이용한 막걸리 제조", 한국식품영양과학회지, 32(6), pp.847~854 (2003), 한국식품영양과학회
  39. Tamura K, "MEGA6 : Molecular evolutionary genetics analysis version 6. 0", Mol Biol Evol, 30, pp.2725~2729 (2013)
  40. Varela C, "Sensory profile and volatile aroma composition of reduced alcohol Merlot wines fermented with Metschnikowia pulcherrima and Saccharomyces uvarum", Int J Food Microbiol, 252, pp.1~9 (2017)
  41. Wei W, "Intergeneric protoplast fusion between Kluyveromyces and Saccharomyces cerevisiae to produce sorbitol from Jerusalem artichokes", Biotechnol Lett, 23, pp.799~803 (2001)
  42. Weng W, "Characterization of edible films based on tilapia(Tilapia zillii)scale gelatin with different extraction pH", Food Hydrocoll, 41, pp.19~26 (2014)
  43. Yuan X, "Free radical scavenging activities and bioactive substances of Jerusalem artichoke (Helianthus tuberosus L.) leaves", Food Chem, 133, pp.10~14 (2012)
  44. Zhang B, "Effects of three indigenous non-Saccharomyces yeasts and their pairwise combinations in co-fermentation with Saccharomyces cerevisiae on volatile compounds of Petit Manseng wines", Food Chem, 368, pp.130807~ (2022)

관련 논문AI추천

돼지감자(Helianthus tuberosus L.)의 첨가량과 증자처리에 따른 약주 발효 특성김찬우, 박희동, 이새벽, 최규택, 최준수한국식품저장유통학회 · Food Science and Preservation2023 · 제30권 제1호전통누룩에서 분리한 non-Saccharomyces cerevisiae의 이화학적 및 기능적 특성김명곤, 김소영, 김순희, 김아라, 여수환, 정우수한국식품저장유통학회 · Food Science and Preservation2022 · 제29권 제3호Non-Saccharomyces와 Saccharomyces cerevisiae Fermivin으로 혼합 발효한 사과와인의 동결농축에 따른 이화학적 특성박희동, 여수환, 위현정, 이새벽, 최규택, 함지연한국식품저장유통학회 · Food Science and Preservation2018 · 제25권 제6호Saccharomyces cerevisiae와 Hanseniaspora uvarum 효모 혼합발효를 이용한 증류식 소주의 휘발성 향기성분 및 아미노산 함량 변화박춘우, 오지윤, 이새벽, 이수현, 이예나, 최규택, 최덕영, 최준수한국식품저장유통학회 · Food Science and Preservation2023 · 제30권 제6호효모를 달리한 약주의 숙성 시 품질 변화강지은, 정석태, 최정실한국식품저장유통학회 · Food Science and Preservation2022 · 제29권 제1호생균제제 효모 Kazachstania exigua CCSY27 및 쌀누룩을 이용하여 제조한 쌀-밀 막걸리의 품질 특성조계만, 주옥수, 황정은한국식품저장유통학회 · Food Science and Preservation2020 · 제27권 제1호

이 간행물의 다른 논문 (14건)