Genotype and Phenotype of Echinococcus granulosus Derived from Wild Sheep (Ovis orientalis) in Iran
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The aim of the present study is to determine the characteristics of genotype and phenotype of Echinococcus granulosus derived from wild sheep and to compare them with the strains of E. granulosus sensu stricto (sheep-dog) and E. granulosus camel strain (camel-dog) in Iran. In Khojir National Park, near Tehran, Iran, a fertile hydatid cyst was recently found in the liver of a dead wild sheep (Ovis orientalis). The number of protoscolices (n=6,000) proved enough for an experimental infection in a dog. The characteristics of large and small hooks of metacestode were statistically determined as the sensu stricto strain but not the camel strain (P=0.5). To determine E. granulosus genotype, 20 adult worms of this type were collected from the infected dog. The second internal transcribed spacer (ITS2) of the nuclear ribosomal DNA (rDNA) and cytochrome c oxidase 1 subunit (COX1) of the mitochondrial DNA were amplified from individual adult worm by PCR. Subsequently, the PCR product was sequenced by Sanger method. The lengths of ITS2 and COX1 sequences were 378 and 857 bp, respectively, for all the sequenced samples. The amplified DNA sequences from both ribosomal and mitochondrial genes were highly similar (99% and 98%, respectively) to that of the ovine strain in the GenBank database. The results of the present study indicate that the morpho-molecular features and characteristics of E. granulosus in the Iranian wild sheep are the same as those of the sheep-dog E. granulosus sensu stricto strain.
Abstract
The aim of the present study is to determine the characteristics of genotype and phenotype of Echinococcus granulosus derived from wild sheep and to compare them with the strains of E. granulosus sensu stricto (sheep-dog) and E. granulosus camel strain (camel-dog) in Iran. In Khojir National Park, near Tehran, Iran, a fertile hydatid cyst was recently found in the liver of a dead wild sheep (Ovis orientalis). The number of protoscolices (n=6,000) proved enough for an experimental infection in a dog. The characteristics of large and small hooks of metacestode were statistically determined as the sensu stricto strain but not the camel strain (P=0.5). To determine E. granulosus genotype, 20 adult worms of this type were collected from the infected dog. The second internal transcribed spacer (ITS2) of the nuclear ribosomal DNA (rDNA) and cytochrome c oxidase 1 subunit (COX1) of the mitochondrial DNA were amplified from individual adult worm by PCR. Subsequently, the PCR product was sequenced by Sanger method. The lengths of ITS2 and COX1 sequences were 378 and 857 bp, respectively, for all the sequenced samples. The amplified DNA sequences from both ribosomal and mitochondrial genes were highly similar (99% and 98%, respectively) to that of the ovine strain in the GenBank database. The results of the present study indicate that the morpho-molecular features and characteristics of E. granulosus in the Iranian wild sheep are the same as those of the sheep-dog E. granulosus sensu stricto strain.
참고문헌 (23)
- Fasihi Harandi M, "Molecular and morphological characterization of Echinococcus granulosus of human and animal origin in Iran", Parasitology, 125, pp.367~373 (2002)
- Eslami A, "Helminth parasites of wild boar, Sus scrofa, in Iran", J Wildl Dis, 28, pp.316~318 (1992)
- Meshgi B, "Prevalence of parasitic infections in the red fox (Vulpes vulpes) and golden jackal (Canis aureus) in Iran", Iran J Vet Res Shiraz Univ, 10, pp.387~391 (2002)
- Sadighian A, "Helminth parasites of stray dogs and jackals in Shahsavar area, Caspian Sea region", J Parasitol, 55, pp.372~374 (1969)
- Hosseini SH, "Morphological and developmental characteristics of Echinococcus granulosus derived of sheep, cattle and camel in Iran", J Helminthol, 72, pp.337~341 (1998)
- Thompson RCA, "Towards a taxonomic revision of the genus Echinococcus", Trend Parasitol, 18, pp.452~457 (2002)
- Zhang L, "Indication of the presence of two distinct strains of Echinococcus granulosus in Iran by mitochondrial DNA markers", Am J Trop Med Hyg, 59, pp.171~174 (1998)
- Nakao M, "A molecular phylogeny of the genus Echinococcus inferred from complete mitochondrial genomes", Parasitology, 134, pp.713~724 (2007)
- Moks E, "First report of Echinococcus granulosus G8 in Eurasia and a reappraisal of the phylogenetic relationships of' ‘genotypes’ G5-G10", Parasitology, 135, pp.647~654 (2008)
- Aminpour A, "Comparative genotyping of Echinococcus granulosus infecting buffalo in Iran using cox1 gene", Parasitol Res, 108, pp.1229~1234 (2011)
- Sharifi I, "Seasonal prevalence of hydatid cyst in slaughterhouse of the city of Kerman", Iran J Public Health, 25, pp.39~46 (1996)
- Daryani A, "The prevalence, intensity and viability of hydatid cyst in slaughtered animals in the Ardabil province ofIran", J Helminthol, 81, pp.13~17 (2007)
- Sharbatkhori M, "Echinococcus granulosus genotypes in livestock of Iran indicating high frequency of G1 genotype in camel", Experiment Parasitol, 124, pp.373~379 (2010)
- Eckert J, "Intraspecific variation of Echinococcus granulosus and related species with emphasis on their infectivity to humans", Acta Trop, 64, pp.19~34 (1997)
- McManus DP, "Echinococcus and Hydatid Disease", pp.135~181 (1995), CAB International
- McManus DP, "Molecular genetic approaches to parasite identification: their value in diagnostic parasitology and systematics", Int J Parasitol, 26, pp.687~704 (1996)
- Hobbs RP, "Rostellar hook morphology of Echinococcus granulosus (Batsch, 1786) from natural and experimental Australian hosts and its implications for strain recognition", Parasitology, 101, pp.273~281 (1990)
- Chilton NB, "Differences in a ribosomal DNA sequence of morphologically indistinguishable species within the Hypodontus macropi complex (Nematoda: Strongyloidea)", Int J Parasitol, 25, pp.647~651 (1995)
- Ahmadi N, "Characterization of Echinococcus granulosus isolates from human, sheep and camel in Iran", Infect Genet Evol, 6, pp.85~90 (2006)
- Eslami A, "Echinococcus granulosus infection of farm dogs of Iran", Parasitol Res, 84, pp.205~207 (1998)
- Himsworth CG, "Emergence of sylvatic Echinococcus granulosus as a parasitic zoonosis of public health concern in an indigenous community in Canada", Am J Trop Med Hyg, 82, pp.643~645 (2010)
- Lavikainen A, "Molecular characterization of Echinococcus isolates of cervid origin from Finland and Sweden", Parasitology, 133, pp.567~570 (2006)
- Claridge AW, "Satellite tracking of wild dogs in south-eastern mainland Australian Forests: implications for management of a problematic top-order carnivore", Forset Ecol Manag, 256, pp.814~822 (2009)