Prevalence of Enteric Protozoan Oocysts with Special Reference to Sarcocystis cruzi among Fecal Samples of Diarrheic Immunodeficient Patients in Iran
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The genus Sarcocystis is not usually considered as an important enteric pathogen in immune compromised patients. It might be expected that species for which humans are the final host (Sarcocystis hominis and Sarcocystis suihominis as well as possibly others) would be encountered increasingly often in immunodeficient persons. This study aimed to address how to detect and differentiate Sarcocystis oocysts and/or sporocysts from enteric protozoans in the diarrheal samples of immunodeficient patients in Shiraz, Iran. Diarrheal samples of 741 immunodeficient patients with recurrent persistent or chronic diarrhea were examined by microscopy and molecular biological analysis. Oocysts-positive samples were 68 Cryptosporidium spp., 9 Cystoisospora belli (syn. Isospora belli), 2 Cyclospora cayetanensis, and 15 microsporidia (Enterocytozoon bieneusi). Sarcocystis-like sporocysts found from a woman were identified as Sarcocystis cruzi through 18S rDNA amplification and phylogenetic analysis. To the best of our knowledge, this is the first report of S. cruzi from a human.
Abstract
The genus Sarcocystis is not usually considered as an important enteric pathogen in immune compromised patients. It might be expected that species for which humans are the final host (Sarcocystis hominis and Sarcocystis suihominis as well as possibly others) would be encountered increasingly often in immunodeficient persons. This study aimed to address how to detect and differentiate Sarcocystis oocysts and/or sporocysts from enteric protozoans in the diarrheal samples of immunodeficient patients in Shiraz, Iran. Diarrheal samples of 741 immunodeficient patients with recurrent persistent or chronic diarrhea were examined by microscopy and molecular biological analysis. Oocysts-positive samples were 68 Cryptosporidium spp., 9 Cystoisospora belli (syn. Isospora belli), 2 Cyclospora cayetanensis, and 15 microsporidia (Enterocytozoon bieneusi). Sarcocystis-like sporocysts found from a woman were identified as Sarcocystis cruzi through 18S rDNA amplification and phylogenetic analysis. To the best of our knowledge, this is the first report of S. cruzi from a human.
참고문헌 (28)
- Dwivedi KK, "Enteric opportunistic parasites among HIV infected individuals : associated risk factors and immune status", Jpn J Infect Dis, 60, pp.76~81 (2007)
- Agholi M, "HIV", AIDS-associated opportunistic protozoal diarrhea, 2013, pp.29, 35~41
- Agholi M, "Microsporidia and coccidia as causes of persistence diarrhea among liver transplant children: incidence rate and species", genotypes, 2013, pp.32, 185~187
- Bhaijee F, "Human immunodeficiency virus-associated gastrointestinal disease: common endoscopic biopsy diagnoses", Pathol Res Int, 2011, pp.247923~ (2011)
- Stark D, "Clinical significance of enteric protozoa in the immunosuppressed human population", Clin Microbiol Rev, 22, pp.634~650 (2009)
- Fayer R, "Human infections with Sarcocystis species", Clin Microbiol Rev, 28, pp.295~311 (2015)
- Poulsen CS, "Current status of epidemiology and diagnosis of human sarcocystosis", J Clin Microbiol, 52, pp.3524~3530 (2014)
- Agholi M, "First detection of acalculous cholecystitis associated with Sarcocystis infection in a patient with AIDS", Acta Parasitol, 59, pp.310~315 (2014)
- Xiang Z, "Non-invasive methods for identifying oocysts of Sarcocystis spp. from definitive hosts", Parasitol Int, 58, pp.293~296 (2009)
- Marcos LA, "Intestinal protozoan infections in the immunocompromised host", Curr Opin Infect Dis, 26, pp.295~301 (2013)
- Danziger-Isakov L, "Gastrointestinal infections after transplantation", Curr Opin Gastroenterol, 30, pp.40~46 (2014)
- Daryani A, "Prevalence of intestinal parasites and profile of CD4+ counts in HIV+", AIDS people in north of Iran, 2007-2008, 2009, pp.12, 1277~1281
- Shafiei R, "Severe diarrhea due to Cystoisospora belli infection in an HTLV-1 woman", Iran J Parasitol, 11, pp.121~125 (2016)
- Nateghi Rostami M, "Isospora belli associated recurrent diarrhea in a child with AIDS", J Parasit Dis, 38, pp.444~446 (2014)
- Mirjalali H, "Genotyping and molecular analysis of Enterocytozoon bieneusi isolated from immunocompromised patients in Iran", Infect Genet Evol, 36, pp.244~249 (2015)
- Wilairatana P, "Intestinal sarcocystosis in Thai laborers", Southeast Asian J Trop Med Public Health, 27, pp.43~46 (1996)
- Oryan A, "The distribution pattern of Sarcocystis species, their transmission and pathogenesis in sheep in Fars Province of Iran", Vet Res Commun, 20, pp.243~453 (1996)
- Shekarforoush SS, "Prevalence of Sarcocystis species in slaughtered goats in Shiraz, Iran", Vet Rec, 156, pp.418~420 (2005)
- Nourollahi-Fard SR, "Prevalence and histopathological finding of thin-walled and thick-walled Sarcocysts in slaughtered cattle of Karaj abattoir, Iran", J Parasit Dis, 39, pp.272~275 (2015)
- Nourani H, "Prevalence of thin-walled Sarcocystis cruzi and thick-walled Sarcocystis hirsuta or Sarcocystis hominis from cattle in Iran", Trop Anim Health Prod, 42, pp.1225~1227 (2010)
- Nourollahi Fard SR, "Survey of Sarcocystis infection in slaughtered cattle in Kerman, Iran", Trop Anim Health Prod, 41, pp.1633~1636 (2009)
- Shekarforoush SS, "Prevalence of Sarcocystis species in slaughtered goats in Shiraz, Iran", Vet Rec, 156, pp.418~420 (2005)
- Oryan A, "Prevalence, biology, and distribution pattern of Sarcocystis infection in water buffalo (Bubalus bubalis) in Iran", Trop Anim Health Prod, 42, pp.1513~1518 (2010)
- Rommel M, "Sarcocystosis of domestic animals and humans", In Pract, 7, pp.158~160 (1985)
- Gjerde B, "Molecular characterisation of three regions of the nuclear ribosomal DNA unit and the mitochondrial cox1 gene of Sarcocystis fusiformis from water buffaloes (Bubalus bubalis) in Egypt", Parasitol Res, 114, pp.3401~3413 (2015)
- Coelho C, "Unraveling Sarcocystis miescheriana and Sarcocystis suihominis infections in wild boar", Vet Parasitol, 212, pp.100~104 (2015)
- Dubey JP, "Sarcocystis caninum and Sarcocystis svanai n. spp. (Apicomplexa: Sarcocystidae) associated with severe myositis and hepatitis in the domestic dog (Canis familiaris)", J Eukaryot Microbiol, 62, pp.307~317 (2015)
- Calero-Bernal R, "In the United States, negligible rates of zoonotic sarcocystosis occur in feral swine that, by contrast, frequently harbour infections with Sarcocystis miescheriana, a related parasite contracted from canids", Parasitology, 142, pp.549~556 (2015)