Epidemiological Characteristics of Strongyloidiasis in Inhabitants of Indigenous Communities in Borneo Island, Malaysia
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Epidemiological study on strongyloidiasis in humans is currently lacking in Malaysia. Thus, a cross-sectional study was carried out to determine the prevalence of Strongyloides stercoralis infection among the inhabitants of longhouse indigenous communities in Sarawak. A single stool and blood sample were collected from each participant and subjected to microscopy, serological and molecular techniques. Five species of intestinal parasites were identified by stool microscopy. None of the stool samples were positive for S. stercoralis. However, 11% of 236 serum samples were seropositive for strongyloidiasis. Further confirmation using molecular technique on stool samples of the seropositive individuals successfully amplified 5 samples, suggesting current active infections. The prevalence was significantly higher in adult males and tended to increase with age. S. stercoralis should no longer be neglected in any intestinal parasitic survey. Combination of more than 1 diagnostic technique is necessary to increase the likelihood of estimating the ‘true’ prevalence of S. stercoralis.
Abstract
Epidemiological study on strongyloidiasis in humans is currently lacking in Malaysia. Thus, a cross-sectional study was carried out to determine the prevalence of Strongyloides stercoralis infection among the inhabitants of longhouse indigenous communities in Sarawak. A single stool and blood sample were collected from each participant and subjected to microscopy, serological and molecular techniques. Five species of intestinal parasites were identified by stool microscopy. None of the stool samples were positive for S. stercoralis. However, 11% of 236 serum samples were seropositive for strongyloidiasis. Further confirmation using molecular technique on stool samples of the seropositive individuals successfully amplified 5 samples, suggesting current active infections. The prevalence was significantly higher in adult males and tended to increase with age. S. stercoralis should no longer be neglected in any intestinal parasitic survey. Combination of more than 1 diagnostic technique is necessary to increase the likelihood of estimating the ‘true’ prevalence of S. stercoralis.
참고문헌 (28)
- Bethony J, "Soil transmitted helminth infections: ascariasis, trichuriasis, and hookworm", Lancet, 367, pp.1521~1532 (2006)
- Hotez PJ, "Incorporating a rapid-impact package for neglected tropical diseases with programs for HIV", AIDS, tuberculosis, and malaria, 2006, pp.3, e102~
- Olsen A, "Strongyloidiasis-the most neglected of the neglected tropical diseases?", Trans R Soc Trop Med Hyg, 103, pp.967~972 (2009)
- Ahmad AF, "Serological and molecular detection of Strongyloides stercoralis infection among Orang Asli community in Malaysia", Parasitol Res, 112, pp.2811~2816 (2013)
- Karim R, "Parasitic infections in the aboriginal community at Temenggor,Hulu Perak, Malaysia", Malay Nat J, 48, pp.425~432 (1995)
- Rahmah N, "Parasitic infections among aborigine children at Post Brooke, Kelantan, Malaysia", Med J Malaysia, 52, pp.412~415 (1997)
- Sithithaworn P, "Epidemiology of Strongyloides stercoralis in northeast Thailand : application of the agar plate culture technique compared with the enzyme-linked immunosorbent assay", Trans R Soc Trop Med Hyg, 97, pp.398~402 (2003)
- Nontasut P, "Prevalence of Strongyloides in Northern Thailand and treatment with ivermectin vs albendazole", Southeast Asian J Trop Med Public Health, 36, pp.442~444 (2005)
- Khieu V, "Prevalence and risk factors of Strongyloides stercoralis in Takeo Province, Cambodia", Parasit Vectors, 7, pp.221~ (2014)
- Sayasone S, "Diversity of human intestinal helminthiasis in Lao PDR", Trans R Soc Trop Med Hyg, 103, pp.247~254 (2009)
- Steinmann P, "Occurrence of Strongyloides stercoralis in Yunnan Province, China, and comparison of diagnostic methods", PLoS Negl Trop Dis, 1, pp.e75~ (2007)
- Ohta N, "Disease burden and epidemiology of soil-transmitted helminthiases and schistosomiasis in Asia : the Japanese perspective", Trends Parasitol, 23, pp.30~35 (2007)
- Schar F, "Strongyloides stercoralis: Global Distribution and Risk Factors", PLoS Negl Trop Dis, 7, pp.e2288~ (2013)
- Ericsson CD, "Diagnosis of Strongyloides stercoralis Infection", Clin Infect Dis, 33, pp.1040~1047 (2001)
- Garcia LS, "Diagnostic Medical Parasitology", pp.791~ (2001), ASM Press
- Koga K, "A modified agar plate method for detection of Strongyloides stercoralis", Am J Trop Med Hyg, 45, pp.518~521 (1991)
- Nilforoushan MR, "A DNA-Based identification of Strongyloides stercoralis isolates from Iran", Iran J Pub Health, 36, pp.16~20 (2007)
- Verweij JJ, "Molecular diagnosis of Strongyloides stercoralis in faecal samples using real-time PCR", Trans R Soc Trop Med Hyg, 103, pp.342~346 (2009)
- Chessbrough M, "Parasitological Test: District Laboratory Practice in Tropical Countries, Part 1" (1998), Cambridge University Press
- Rayan HZ, "Detection of Strongyloides stercoralis in fecal samples using conventional parasitological techniques and Real-Time PCR : a comparative study", Parasitol United J, 5, pp.27~34 (2012)
- Liu LX, "Strongyloidiasis and other intestinal nematode infections", Infect Dis Clin North Am, 7, pp.655~682 (1993)
- Paula FM, "Epidemiological aspects of strongyloidiasis in Brazil", Parasitology, 138, pp.1331~1340 (2011)
- Norsyahida A, "Laboratory detection of strongyloidiasis: IgG-, IgG4 - and IgE-ELISAs and cross-reactivity with lymphatic filariasis", Parasite Immunol, 35, pp.174~179 (2013)
- Yori PP, "Seroepidemiology of strongyloidiasis in the Peruvian Amazon", Am J Trop Med Hyg, 74, pp.97~102 (2006)
- Hall A, "Strongyloides stercoralis in an urban slum community in Bangladesh : factors independently associated with infection", Trans R Soc Trop Med Hyg, 88, pp.527~530 (1994)
- Wu TC, "Strongyloides stercoralis infection : a health issue regarding indigenous people in Taiwan", Trans R Soc Trop Med Hyg, 106, pp.468~472 (2012)
- Traub RJ, "The role of dogs in transmission of gastrointestinal parasites in a remote tea-growing community in northeastern India", Am J Trop Med Hyg, 67, pp.539~545 (2002)
- Anderson RM, "Infectious diseases of humans: dynamics and control" (1992), Oxford University Press