Immune Correlates of Resistance to Trichinella spiralis Reinfection in Mice
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The immune correlate of host resistance induced by reinfection of Trichinella spiralis remains unclear. In this study, we investigated immune correlates between the resistance and serum IgG antibody level, CD23+ IgM+ B cells, and eosinophil responses induced by T. spiralis reinfection. Mice were primarily infected with 10 or 100 T. spiralis larvae (10 TS, 100 TS), respectively, and after 4 weeks, they were challenge infected with 100 T. spiralis larvae (10-100 TS, 100-100 TS). Upon challenge infections, 10-100 TS mice induced significantly higher levels of T. spiralis-specific total IgG antibody responses in sera and antibody secreting cell responses in spleens compared to 100-100 TS mice, resulting in significantly reduced worm burdens in 10-100 TS mice (60% and 70% reductions for adult and larvae, respectively). Higher levels of eosinophils were found in mice primarily infected with 10 TS compared to those of 100 TS at week 8 upon challenge. CD23+ IgM+ B cells were found to be increased significantly in mice primarily infected with 10 TS. These results indicate that primary infection of 10 larvae of T. spiralis, rather than 100 larvae, induces significant resistance against reinfection which closely correlated with T. spiralis-specific IgG, eosinophil, and CD23+ IgM+ B cell responses.
Abstract
The immune correlate of host resistance induced by reinfection of Trichinella spiralis remains unclear. In this study, we investigated immune correlates between the resistance and serum IgG antibody level, CD23+ IgM+ B cells, and eosinophil responses induced by T. spiralis reinfection. Mice were primarily infected with 10 or 100 T. spiralis larvae (10 TS, 100 TS), respectively, and after 4 weeks, they were challenge infected with 100 T. spiralis larvae (10-100 TS, 100-100 TS). Upon challenge infections, 10-100 TS mice induced significantly higher levels of T. spiralis-specific total IgG antibody responses in sera and antibody secreting cell responses in spleens compared to 100-100 TS mice, resulting in significantly reduced worm burdens in 10-100 TS mice (60% and 70% reductions for adult and larvae, respectively). Higher levels of eosinophils were found in mice primarily infected with 10 TS compared to those of 100 TS at week 8 upon challenge. CD23+ IgM+ B cells were found to be increased significantly in mice primarily infected with 10 TS. These results indicate that primary infection of 10 larvae of T. spiralis, rather than 100 larvae, induces significant resistance against reinfection which closely correlated with T. spiralis-specific IgG, eosinophil, and CD23+ IgM+ B cell responses.
참고문헌 (30)
- 김가연, "An Outbreak of Trichinellosis with Detection of Trichinella Larvae in Leftover Wild Boar Meat", Journal of Korean Medical Science, 26(12), pp.1630~1633 (2011), 대한의학회
- Gottstein B, "Epidemiology, diagnosis, treatment, and control of trichinellosis", Clin Microbiol Rev, 22, pp.127~145 (2009)
- Kolodziej-Sobocinska M, "Trichinella spiralis reinfection : macrophage activity in BALB", c mice, 2007, pp.101, 629~637
- Grove DI, "Kinetics of immunological responses, resistance to reinfection, and pathological reactions to infection with Trichinella spiralis", J Infect Dis, 136, pp.562~570 (1977)
- Franssen FF, "Antibody response against Trichinella spiralis in experimentally infected rats is dose dependent", Vet Res, 42, pp.113~ (2011)
- Smith HJ, "Pathogenesis and serodiagnosis of experimental Trichinella spiralis spiralis and Trichinella spiralis nativa infections in cattle", Can J Vet Res, 54, pp.355~359 (1990)
- Tomasovicova O, "Experimental Trichinella spiralis infection in sheep", Vet Parasitol, 40, pp.119~126 (1991)
- Pajersky A, "Susceptibility and reactivity of sheep to Trichinella spiralis infection", Vet Med, 41, pp.233~240 (1996)
- Reina D, "Experimental trichinellosis in goats", Vet Parasitol, 62, pp.125~132 (1996)
- Korinkova K, "Distribution of muscle larvae and antibody dynamics in goats experimentally infected with Trichinella spiralis", Parasitol Res, 99, pp.643~647 (2006)
- Smith HJ, "Experimental Trichinella infections in ponies", Can J Vet Res, 51, pp.415~416 (1987)
- Soule C, "Biological and parasitic variations in horses infested and reinfested by Trichinella spiralis", Vet Res, 24, pp.21~31 (1993)
- Pozio E, "Evaluation of ELISA and western blot analysis using three antigens to detect anti-Trichinella IgG in horses", Vet Parasitol, 108, pp.163~178 (2002)
- Moller LN, "Comparison of two antigens for demonstration of Trichinella spp. antibodies in blood and muscle fluid of foxes, pigs and wild boars", Vet Parasitol, 132, pp.81~84 (2005)
- Nockler K, "Experimental studies in pigs on Trichinella detection in different diagnostic matrices", Vet Parasitol, 132, pp.85~90 (2005)
- Korinkova K, "Serological detection of Trichinella spiralis in swine by ELISA(enzyme-linked immunosorbent assay)using an excretory-secretory(E", S)antigen, 2008, pp.102, 1317~1320
- Dvoroznakova E, "Trichinella spiralis reinfection : Changes in cellular and humoral immune response in BALB", c mice, 2012, pp.49, 201~210
- Dvoroznakova E, "Differences in cellular immune responses of mice BALB", c to low and high infective doses of Trichinella spiralis, 2013, pp.50, 244~253
- Huang L, "Eosinophils mediate protective immunity against secondary nematode infection", J Immunol, 194, pp.283~290 (2015)
- Mwinzi PN, "Circulating CD23+ B cell subset correlates with the development of resistance to Schistosoma mansoni reinfection in occupationally exposed adults who have undergone multiple treatments", J Infect Dis, 199, pp.272~279 (2009)
- Reynaud CA, "IgM memory B cells : A mouse", human paradox, 2012, pp.69, 1625~1634
- Woon-Mok SOHN, "Molecular identification of Korean Trichinella isolates", The Korean Journal of Parasitology, 41(2), pp.125~127 (2003), 대한기생충학ㆍ열대의학회
- Quan FS, "Relationships between IgG, IgM, IgE and resistance to reinfection during the early phase of infection with Clonorchis sinensis in rats", Immunol Invest, 33, pp.51~60 (2004)
- Robinson K, "Immunity to Trichinella spiralis transferred by serum from vaccinated mice not protected by immunization", Parasite Immunol, 17, pp.85~90 (1995)
- Quan FS, "Virus-like particle vaccine protects against 2009 H1N1 pandemic influenza virus in mice", PLoS One, 5, pp.e9161~ (2010)
- Karanja DM, "Resistance to reinfection with Schistosoma mansoni in occupationally exposed adults and effect of HIV-1 co-infection on susceptibility to schistosomiasis : A longitudinal study", Lancet, 360, pp.592~596 (2002)
- Blackwell NM, "B cells and antibodies are required for resistance to the parasitic gastrointestinal nematode Trichuris muris", Infect Immun, 69, pp.3860~3868 (2001)
- McVay CS, "Participation of parasite surface glycoproteins in antibody-mediated protection of epithelial cells against Trichinella spiralis", Infect Immun, 66, pp.1941~1945 (1998)
- McVay CS, "Antibodies to tyvelose exhibit multiple modes of interference with the epithelial niche of Trichinella spiralis", Infect Immun, 68, pp.1912~1918 (2000)
- Alba-Hurtado F, "Immune responses associated with resistance to haemonchosis in sheep", Biomed Res Int, 2013, pp.162158~ (2013)