Prevalence of Hymenolepis nana and H. diminuta from Brown Rats (Rattus norvegicus) in Heilongjiang Province, China
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Hymenolepis nana and Hymenolepis diminuta are globally widespread zoonotic cestodes. Rodents are the main reservoir host of these cestodes. Brown rats (Rattus norvegicus) are the best known and most common rats, and usually live wherever humans live, especially in less than desirable hygiene conditions. Due to the little information of the 2 hymenolepidid species in brown rats in China, the aim of this study was to understand the prevalence and genetic characterization of H. nana and H. diminuta in brown rats in Heilongjiang Province, China. Total 114 fecal samples were collected from brown rats in Heilongjiang Province. All the samples were subjected to morphological examinations by microscopy and genetic analysis by PCR amplification of the mitochondrial cytochrome c oxidase subunit 1 (COX1) gene and the internal transcribed spacer 2 (ITS2) region of the nuclear ribosomal RNA gene. In total, 6.1% (7/114) and 14.9% (17/114) of samples were positive for H. nana and H. diminuta, respectively. Among them, 7 and 3 H. nana isolates were successfully amplified and sequenced at the COX1 and ITS2 loci, respectively. No nucleotide variations were found among H. nana isolates at either of the 2 loci. Seventeen H. diminuta isolates produced 2 different COX1 sequences while 7 ITS2 sequences obtained were identical to each other. The present results of H. nana and H. diminuta infections in brown rats implied the risk of zoonotic transmission of hymenolepiasis in China. These molecular data will be helpful to deeply study intra-specific variations within Hymenolepis cestodes in the future.
Abstract
Hymenolepis nana and Hymenolepis diminuta are globally widespread zoonotic cestodes. Rodents are the main reservoir host of these cestodes. Brown rats (Rattus norvegicus) are the best known and most common rats, and usually live wherever humans live, especially in less than desirable hygiene conditions. Due to the little information of the 2 hymenolepidid species in brown rats in China, the aim of this study was to understand the prevalence and genetic characterization of H. nana and H. diminuta in brown rats in Heilongjiang Province, China. Total 114 fecal samples were collected from brown rats in Heilongjiang Province. All the samples were subjected to morphological examinations by microscopy and genetic analysis by PCR amplification of the mitochondrial cytochrome c oxidase subunit 1 (COX1) gene and the internal transcribed spacer 2 (ITS2) region of the nuclear ribosomal RNA gene. In total, 6.1% (7/114) and 14.9% (17/114) of samples were positive for H. nana and H. diminuta, respectively. Among them, 7 and 3 H. nana isolates were successfully amplified and sequenced at the COX1 and ITS2 loci, respectively. No nucleotide variations were found among H. nana isolates at either of the 2 loci. Seventeen H. diminuta isolates produced 2 different COX1 sequences while 7 ITS2 sequences obtained were identical to each other. The present results of H. nana and H. diminuta infections in brown rats implied the risk of zoonotic transmission of hymenolepiasis in China. These molecular data will be helpful to deeply study intra-specific variations within Hymenolepis cestodes in the future.
참고문헌 (30)
- Cheng T, "The complete mitochondrial genome of the dwarf tapeworm Hymenolepis nana—a neglected zoonotic helminth", Parasitol Res, 115, pp.1253~1262 (2016)
- Thompson RC, "Neglected zoonotic helminths: Hymenolepis nana, Echinococcus canadensis and Ancylostoma ceylanicum", Clin Microbiol Infect, 21, pp.426~432 (2015)
- Crompton DW, "How much human helminthiasis is there in the world?", J Parasitol, 85, pp.397~403 (1999)
- Tena D, "Human infection with Hymenolepis diminuta : case report from Spain", J Clin Microbiol, 36, pp.2375~2376 (1998)
- Karuna T, "A case of Hymenolepis diminuta in a young male from Odisha", Trop Parasitol, 3, pp.145~147 (2013)
- Kim, Bong Jin, "Heavy Hymenolepis nana infection possibly through organic foods: report of a case", The Korean Journal of Parasitology, 52(1), pp.85~87 (2014), 대한기생충학ㆍ열대의학회[DOI]
- Olson PD, "Lethal invasive cestodiasis in immunosuppressed patients", J Infect Dis, 187, pp.1962~1966 (2003)
- Muehlenbachs A, "Malignant transformation of Hymenolepis nana in a human host", N Engl J Med, 373, pp.1845~1852 (2015)
- Franssen F, "Helminth parasites in black rats(Rattus rattus)and brown rats(Rattus norvegicus)from different environments in the Netherlands", Infect Ecol Epidemiol, 6, pp.31413~ (2016)
- Simões RO, "Biotic and abiotic effects on the intestinal helminth community of the brown rat Rattus norvegicus from Rio de Janeiro, Brazil", J Helminthol, 90, pp.21~27 (2016)
- Tung KC, "Study of the endoparasitic fauna of commensal rats and shrews caught in traditional wet markets in Taichung City, Taiwan", J Microbiol Immunol Infect, 46, pp.85~88 (2013)
- d'Ovidio D, "Survey of Hymenolepis spp. in pet rodents in Italy", Parasitol Res, 114, pp.4381~4384 (2015)
- Kataranovski M, "Intestinal helminthes infection of rats (Ratus norvegicus) in the Belgrade area (Serbia): the effect of sex, age and habitat", Parasite, 18, pp.189~196 (2011)
- Malsawmtluangi C, "Helminth parasite spectrum in rodent hosts from bamboo growing areas of Mizoram, North-east India", J Parasit Dis, 33, pp.28~35 (2009)
- Wu J, "Investigation on infection of Hymenolepis among Rattus noruegicus and Rattus flavipectus in Zhanjiang city, Guangdong province", Chin J Parasit Dis Con, 17, pp.306~307 (2004)
- Li BS, "Preliminary studies on the correlativity of Hymenolepis nana and Hymenolepis murina infection in Rural Communities, Xinjiang", Endemic Dis Bulletin, 18, pp.15~17 (2003)
- Nkouawa A, "Cryptic diversity in hymenolepidid tapeworms infecting humans", Parasitol Int, 65, pp.83~86 (2016)
- Sharma S, "Differential diagnosis and molecular characterization of Hymenolepis nana and Hymenolepis diminuta(Cestoda : Cyclophyllidea : Hymenolepididae)based on nuclear rDNA ITS2 gene marker", Parasitol Res, 115, pp.4293~4298 (2016)
- Qu H, "Important investigation of zoonotic parasites in Heilongjiang Province", Agricult Technol, 19, pp.33~36 (1999)
- Bessho Y, "Planarian mitochondria. I. Heterogeneity of cytochrome coxidase subunit I gene sequences in the freshwater planarian, Dugesia japonica", J Mol Evol, 34, pp.324~330 (1992)
- Blair D, "Relationships between Schistosoma malayensis and other Asian schistosomes deduced from DNA sequences", Mol Biochem Parasitol, 85, pp.259~263 (1997)
- Steinmann P, "FLOTAC for the diagnosis of Hymenolepis spp. infection: proof-of-concept and comparing diagnostic accuracy with other methods", Parasitol Res, 111, pp.749~754 (2012)
- Mirjalali H, "Molecular analysis of isolates of the cestode Rodentolepis nana from the great gerbil, Rhombomys opimus", J Helminthol, 90, pp.252~255 (2016)
- Macnish MG, "A molecular phylogeny of nuclear and mitochondrial sequences in Hymenolepis nana (Cestoda) supports the existence of a cryptic species", Parasitology, 125, pp.567~575 (2002)
- Okamoto M, "Phylogenetic relationships of three hymenolepidid species inferred from nuclear ribosomal and mitochondrial DNA sequences", Parasitology, 115, pp.661~666 (1997)
- Mohammadzadeh T, "Study on the genomic diversity of Hymenolepis nana between rat and mouse isolates by RAPD-PCR", Iran J Vet Res, 8, pp.16~19 (2007)
- Cheng T, "Genetic variability among Hymenolepis nana isolates from different geographical regions in China revealed by sequence analysis of three mitochondrial genes", Mitochondrial DNA A DNA Mapp Seq Anal, 27, pp.4646~4650 (2016)
- Foronda P, "Distribution and genetic variation of hymenolepidid cestodes in murid rodents on the Canary Islands(Spain)", Parasit Vectors, 26, pp.185~ (2011)
- Kang QD, "An evaluation on survey of human parasite distribution in Heilongjiang Province", Chin J Parasitol Parasit Dis, S1, pp.61~63 (1994)
- Shi FP, "A case of Hymenolepis diminuta in human", J Jiamusi Med Coll, 12 (1989)