Seroprevalence of Toxoplasmosis with ELISA and Rapid Diagnostic Test among Residents in Gyodong-do, Inchon city, Korea: A Four-Year Follow-up
2
조회
0
다운로드
5
인용
0
참고문헌
키워드
초록
ELISA has been used for the diagnosis of toxoplasmosis, but it is being gradually replaced by a rapid diagnostic test (RDT). We compared and analyzed ELISA and RDT results using the sera collected during 4 consecutive years from residents of Gyodong-do (Island), Incheon-city, Korea. Sera from 921, 993, 940, and 838 adult residents were collected on a yearly basis (2010-2013). ELISA was performed by using a crude extract of T. gondii RH strain antigen and IgG/IgM RDT mounted with recombinant fragment of major surface antigen (SAG1), GST-linker-SAG1A, were applied to the sera. Comparison between groups was analyzed by the Student’s t-test. The positive seroprevalence surged from 14.7% (135/921, 2010), 23.1% (231/993, 2011), 23.6% (222/940, 2012), and 32.1% (269/838, 2013) by ELISA. In contrast, RDT showed a more moderate increasing trend from 21.7% (200/921, 2010), 25.5% (253/993, 2011), 28.9% (272/940, 2012) and 33.1% (277/838, 2013). Discrepancies between ELISA and RDT were noted near the cut-off value. At the OD 0.15-0.24 range, RDT could detect 16.1% (169/1051) more positives, which suggests an early or acute toxoplasmosis, but at the OD 0.25-0.34 range, ELISA could detect 35.9% (92/256) more positives of possible chronic infections. Over the OD > 0.35 ELISA and RDT agreed in the majority of the cases. This surge in seroprevalence may be caused by the organic agriculture in addition to eating behavior or increase in pets among Koreans. These facts may be applied on a full-scale national survey using RDT to supplement ELISA to define the characteristics of the infection.
Abstract
ELISA has been used for the diagnosis of toxoplasmosis, but it is being gradually replaced by a rapid diagnostic test (RDT). We compared and analyzed ELISA and RDT results using the sera collected during 4 consecutive years from residents of Gyodong-do (Island), Incheon-city, Korea. Sera from 921, 993, 940, and 838 adult residents were collected on a yearly basis (2010-2013). ELISA was performed by using a crude extract of T. gondii RH strain antigen and IgG/IgM RDT mounted with recombinant fragment of major surface antigen (SAG1), GST-linker-SAG1A, were applied to the sera. Comparison between groups was analyzed by the Student’s t-test. The positive seroprevalence surged from 14.7% (135/921, 2010), 23.1% (231/993, 2011), 23.6% (222/940, 2012), and 32.1% (269/838, 2013) by ELISA. In contrast, RDT showed a more moderate increasing trend from 21.7% (200/921, 2010), 25.5% (253/993, 2011), 28.9% (272/940, 2012) and 33.1% (277/838, 2013). Discrepancies between ELISA and RDT were noted near the cut-off value. At the OD 0.15-0.24 range, RDT could detect 16.1% (169/1051) more positives, which suggests an early or acute toxoplasmosis, but at the OD 0.25-0.34 range, ELISA could detect 35.9% (92/256) more positives of possible chronic infections. Over the OD > 0.35 ELISA and RDT agreed in the majority of the cases. This surge in seroprevalence may be caused by the organic agriculture in addition to eating behavior or increase in pets among Koreans. These facts may be applied on a full-scale national survey using RDT to supplement ELISA to define the characteristics of the infection.
참고문헌 (30)
- Black MW, "Lytic cycle of Toxoplasma gondii", Microbiol Mol Biol Rev, 64, pp.607~623 (2000)
- Furtado JM, "Toxoplasmosis : a global threat", J Glob Infect Dis, 3, pp.281~284 (2011)
- Tenter AM, "Toxoplasma gondii : from animals to humans", Int J Parasitol, 30, pp.1217~1258 (2000)
- Montoya JG, "Management of Toxoplasma gondii infection during pregnancy", Clin Infect Dis, 47, pp.554~566 (2008)
- 송경주, "Seroprevalence of toxoplasmosis in Korean pregnant women", The Korean Journal of Parasitology, 43(2), pp.1~10 (2005), 대한기생충학ㆍ열대의학회
- Weiss LM, "Toxoplasmosis: A history of clinical observations", Int J Parasitol, 39, pp.895~901 (2009)
- Choi WY, "Foodborne outbreaks of human toxoplasmosis", J Infect Dis, 175, pp.1280~1282 (1997)
- Kim MH, "A toxoplasmic uveitis case of a 60-year-old male in Korea", Korean J Parasitol, 38, pp.29~31 (2000)
- 박영훈, "Clinical Features of Ocular Toxoplasmosis in Korean Patients", The Korean Journal of Parasitology, 49(2), pp.167~171 (2011), 대한기생충학ㆍ열대의학회
- Tsubota N, "Studies on latex agglutination test for toxoplasmosis. 2. Evaluation the microtiter test as a serological test for toxoplasmosis in man", Jpn J Parasitol, 26, pp.286~290 (1977)
- Kobayashi A, "Evaluaion of a commercial Toxoplasma latex agglutination test", Jpn J Parasitol, 26, pp.175~180 (1977)
- Choi WY, "Toxoplasma antibody titers by indirect latex agglutination test in patients of Kangnam St. Mary's hospital and Cheju medical center", Korean J Parasitol, 27, pp.171~175 (1989)
- Choi WY, "Detection of antibodies in serum and cerebrospinal fluid to Toxoplasma gondii by indirect latex agglutination test and enzyme-linked immunosorbent assay", Korean J Parasitol, 30, pp.83~90 (1992)
- 홍성종, "Maintained Seroprevalence of Toxoplasmosis among the Residents of Jeju Island, Korea", The Korean Journal of Parasitology, 49(3), pp.309~311 (2011), 대한기생충학ㆍ열대의학회
- Yang HJ, "Seroprevalence of toxoplasmosis in the residents of Cheju island, Korea", Korean J Parasitol, 38, pp.91~93 (2000)
- 안혜진, "Seroprevalence of Toxoplasmosis in the Residents of Cheorwon-gun, Gangwon-do, Korea", The Korean Journal of Parasitology, 50(3), pp.225~227 (2012), 대한기생충학ㆍ열대의학회
- Choi WY, "Toxoplasmacidal effect of HL-60 cells differentiated by dimethylsulfoxide", Korean J Parasitol, 26, pp.229~238 (1988)
- Sohn WM, "Western blot analysis of stray cat sera against Toxoplasma gondii and the diagnostic availability of monoclonal antibodies in sandwich-ELISA", Korean J Parasitol, 37, pp.249~256 (1999)
- 송경주, "A Rapid Diagnostic Test for Toxoplasmosis using Recombinant Antigenic N-terminal Half of SAG1 Linked with Intrinsically Unstructured Domain of GRA2 Protein", The Korean Journal of Parasitology, 51(5), pp.503~510 (2013), 대한기생충학ㆍ열대의학회
- Soh CT, "Latent infection by Toxoplasma gondii in Korea", Yonsei Med J, 1, pp.52~54 (1960)
- Dubey JP, "High prevalence and genotypes of Toxoplasma gondii isolated from organic pigs in northern USA", Vet Parasitol, 188, pp.14~18 (2012)
- Meerburg BG, "Neospora caninum and Toxoplasma gondii in brain tissue of feral rodents and insectivores caught on farms in the Netherlands", Vet Parasitol, 184, pp.317~320 (2012)
- Kim KC, "Preserving biodiversity in Korea's demilitarized zone", Science, 278, pp.242~243 (1997)
- Deok-Gyu Kim, "Intestinal Nematodes from Small Mammals Captured near the Demilitarized Zone, Gyeonggi Province, Republic of Korea", The Korean Journal of Parasitology, 53(1), pp.135~139 (2015), 대한기생충학ㆍ열대의학회
- André MR1, "Antibodies to Toxoplasma gondii and Neospora caninum in captive neotropical and exotic wild canids and felids", J Parasitol, 96, pp.1007~1009 (2010)
- McIntyre KM, "Using open-access taxonomic and spatial information to create a comprehensive database for the study of mammalian and avian livestock and pet infections", Prev Vet Med, 116, pp.325~335 (2014)
- Tilahun G, "Prevalence of Toxoplasma gondii from free-range chickens (Gallus domesticus) from Addis Ababa, Ethiopia", J Parasitol, 99, pp.740~741 (2013)
- Zhaoshou Yang, "A Surge in the Seroprevalence of Toxoplasmosis among the Residents of Islands in Gangwha-gun, Incheon, Korea", The Korean Journal of Parasitology, 50(3), pp.191~197 (2012), 대한기생충학ㆍ열대의학회
- Kim JY, "Further evidence of increasing diversity of Plasmodium vivax in the Republic of Korea in recent years", PLoS One, 11, pp.e0151514~ (2016)
- Young Eui Jeong, "Comparison of the Epidemiological Aspects of Imported Dengue Cases between Korea and Japan, 2006-2010", Osong Public Health and Research Persptectives, 7(1), pp.71~74 (2016), 질병관리본부