Hypolobocera guayaquilensis (Decapoda: Pseudothelphusidae): A New Crab Intermediate Host of Paragonimus mexicanus in Manabi Province, Ecuador
0
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
To determine that Paragonimus sp. is actively transmitted in a tropical area of the Pacific region of Ecuador where human cases of pulmonary paragonimiasis have recently been documented, a total of 75 freshwater crabs were collected from 2 different streams in the Pedernales area of Manabi Province, Ecuador. All collected crabs were identified as Hypolobocera guayaquilensis based on morphological characteristics of the male gonopods. The hepatopancreas of each crab was examined by compressing it between 2 glass plates followed by observation under a stereomicroscope. Excysted Paragonimus metacercariae were detected in 39 (52.0%) crabs and their densities varied from 1 to 32 per infected crab. There was a positive relationship between crab size and metacercarial density. Sequences of the second internal transcribed spacer region of the ribosomal RNA gene of the Paragonimus metacercariae obtained in this study were identical to those of Paragonimus mexicanus deposited in the DDBJ/EMBL/GenBank database. Thus, the present study is the first to confirm that the crab species H. guayaquilensis is the second intermediate host of P. mexicanus in Manabi Province, Ecuador. Because this crab might be the possible source of human infections in this area, residents should pay attention to improper crab-eating habits related with a neglected parasitic disease, i.e., paragonimiasis.
Abstract
To determine that Paragonimus sp. is actively transmitted in a tropical area of the Pacific region of Ecuador where human cases of pulmonary paragonimiasis have recently been documented, a total of 75 freshwater crabs were collected from 2 different streams in the Pedernales area of Manabi Province, Ecuador. All collected crabs were identified as Hypolobocera guayaquilensis based on morphological characteristics of the male gonopods. The hepatopancreas of each crab was examined by compressing it between 2 glass plates followed by observation under a stereomicroscope. Excysted Paragonimus metacercariae were detected in 39 (52.0%) crabs and their densities varied from 1 to 32 per infected crab. There was a positive relationship between crab size and metacercarial density. Sequences of the second internal transcribed spacer region of the ribosomal RNA gene of the Paragonimus metacercariae obtained in this study were identical to those of Paragonimus mexicanus deposited in the DDBJ/EMBL/GenBank database. Thus, the present study is the first to confirm that the crab species H. guayaquilensis is the second intermediate host of P. mexicanus in Manabi Province, Ecuador. Because this crab might be the possible source of human infections in this area, residents should pay attention to improper crab-eating habits related with a neglected parasitic disease, i.e., paragonimiasis.
참고문헌 (16)
- Acha P, "Zoonosis y Enfermedades Transmisibles Comunes al Hombre y a los Animales: Volumen III. Parasitosis", Organizacion Panamericana de la Salud, 158~164 (2003)
- Iwagami M, "A molecular phylogeographic study based on DNA sequences from individual metacercariae of Paragonimus mexicanus from Guatemala and Ecuador", J Helminthol, 77, pp.33~38 (2003)[DOI]
- Waikagul J, "Paragonimus infection in Pedernales, Ecuador", Bull Central Res Inst Fukuoka Univ, Series E, Interdisc Sci, 1, pp.259~273 (2003)
- Miyazaki I, "An illustrated book of helminthic zoonoses", pp.125~129 (1991), Southeast Asian Medical Information Center
- Lopez-Caballero J, "Detection of multiple species of human Paragonimus from Mexico using morphological data and molecular barcodes", Mol Ecol Resour, 13, pp.1125~1136 (2013)
- Voelker J, "Ein neuer Lungenegel aus der Kustenkordillere von Ecuador: Paragonimus ecuadoriensis n. sp. (Paragoninidae; Trematoda)", Tropenmed Parasit, 30, pp.249~263 (1979)
- Calvopina M, "Current status of Paragonimus and paragonimiasis in Ecuador", Mem Inst Oswaldo Cruz, 109, pp.849~855 (2014)[DOI]
- Ministerio del Ambiente del Ecuador (MAE), "Sistema de Clasificacion de los Ecosistemas del Ecuador Continental", Subsecretaria de Patrimonio Natural, ~ (2012)
- Sugiyama H, "Molecular Detection of Human Parasitic Pathogens", pp.423~436 (2013), CRC Press
- Vieira JC, "Paragonimiasis in Ecuador: prevalence and geographical distribution of parasitisation of second intermediate hosts with Paragonimus mexicanus in Esmeraldas Province", Trop Med Parasitol, 43, pp.249~252 (1992)
- Bowles J, "A molecular phylogeny of the human schistosomes", Mol Phylog Evol, 4, pp.103~109 (1995)[DOI]
- Blair D, "Geographical genetic structure within the human lung fluke, Paragonimus westermani, detected from DNA sequences", Parasitology, 115, pp.411~417 (1997)[DOI]
- Fischer PU, "Molecular characterization of the North American lung fluke Paragonimus kellicotti in Missouri and its development in Mongolian gerbils", Am J Trop Med Hyg, 84, pp.1005~1011 (2011)[DOI]
- Calvopina M, "Severe pleuropulmonary paragonimiasis caused by Paragonimus mexicanus treated as tuberculosis in Ecuador", Am J Trop Med Hyg, 96, pp.97~99 (2017)[DOI]
- Takeda M, "Recent collections of freshwater crabs from the Pacific and Amazonian regions of Ecuador, South America", J Teikyo Heisei Univ, 27, pp.1~16 (2016)
- Hernandez-Chea R, "Morphological and molecular characterization of the metacercaria of Paragonimus caliensis, as a separate species from P. mexicanus in Costa Rica", Parasitol Int, 66, pp.126~133 (2017)[DOI]