Distribution Status of Hybrid Types in Large Liver Flukes, Fasciola Species (Digenea: Fasciolidae), from Ruminants and Humans in Vietnam
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
The aim of this study is to delineate ‘admixed hybrid’ and ‘introgressive’ Fasciola genotypes present in the Fas- ciola population in Vietnam. Adult liver flukes collected from ruminants in 18 Provinces were morphologically sorted out by naked eyes for small (S), medium (M) and large (L) body shapes; and human samples (n = 14) from patients. Nuclear ri- bosomal (rDNA) ITS1 and ITS2, and mitochondrial (mtDNA) nad1 markers were used for determination of their genetic status. Total 4,725 worm samples of ruminants were tentatively classified by their size: 6% (n=284) small (S)-, 13% (n=614) medium (M)-, and 81% (n=3,827) large (L)-forms. All the representative (n=120, as 40 each group) and 14 hu- man specimens, possessed maternal mtDNA of only F. gigantica and none of F. hepatica. Paternally, all (100%) of the L- (n=40) and 77.5% (n=31) of the M-flukes had single F. gigantica rDNA indicating ‘pure’ F. gigantica. A majority (90%, n = 36) of the S- and 15% (n = 6) of the M-worms had single F. hepatica rDNA, indicating their introgressive; the rest (10%, n = 4) of the S- and 7.5% (n = 3) of the M-flukes had mixture of both F. gigantica and F. hepatica rDNAs, confirming their admixed hybrid genetic status. Fourteen human samples revealed 9 (64%) of pure F. gigantica, 3 (22%) of introgressive and 2 (14%) of admixed hybrid Fasciola spp. By the present study, it was confirmed that the small worms, which are mor- phologically identical with F. hepatica, are admixed and/or introgressive hybrids of Fasciola spp., and able to be the pathogens of human fascioliasis.
Abstract
The aim of this study is to delineate ‘admixed hybrid’ and ‘introgressive’ Fasciola genotypes present in the Fas- ciola population in Vietnam. Adult liver flukes collected from ruminants in 18 Provinces were morphologically sorted out by naked eyes for small (S), medium (M) and large (L) body shapes; and human samples (n = 14) from patients. Nuclear ri- bosomal (rDNA) ITS1 and ITS2, and mitochondrial (mtDNA) nad1 markers were used for determination of their genetic status. Total 4,725 worm samples of ruminants were tentatively classified by their size: 6% (n=284) small (S)-, 13% (n=614) medium (M)-, and 81% (n=3,827) large (L)-forms. All the representative (n=120, as 40 each group) and 14 hu- man specimens, possessed maternal mtDNA of only F. gigantica and none of F. hepatica. Paternally, all (100%) of the L- (n=40) and 77.5% (n=31) of the M-flukes had single F. gigantica rDNA indicating ‘pure’ F. gigantica. A majority (90%, n = 36) of the S- and 15% (n = 6) of the M-worms had single F. hepatica rDNA, indicating their introgressive; the rest (10%, n = 4) of the S- and 7.5% (n = 3) of the M-flukes had mixture of both F. gigantica and F. hepatica rDNAs, confirming their admixed hybrid genetic status. Fourteen human samples revealed 9 (64%) of pure F. gigantica, 3 (22%) of introgressive and 2 (14%) of admixed hybrid Fasciola spp. By the present study, it was confirmed that the small worms, which are mor- phologically identical with F. hepatica, are admixed and/or introgressive hybrids of Fasciola spp., and able to be the pathogens of human fascioliasis.
참고문헌 (39)
- Mas-Coma S, "Chapter 2. Fasciola, lymnaeids and human fascioliasis, with a global overview on disease transmission, epidemiology, evolutionary genetics, molecular epidemiology and control", Adv Parasitol, 69, pp.41~146 (2009)
- Cwiklinski K, "A prospective view of animal and human fasciolosis", Parasite Immunol, 38, pp.558~568 (2016)
- Lin RQ, "Sequence analysis of the first internal transcribed spacer of rDNA supports the existence of the intermediate Fasciola between F. hepatica and F. gigantica in mainland China", Parasitol Res, 101, pp.813~817 (2007)
- Peng M, "Molecular characterization of Fasciola hepatica, Fasciola gigantica, and aspermic Fasciola sp. in China based on nuclear and mitochondrial DNA", Parasitol Res, 105, pp.809~815 (2009)
- Ai L, "Genetic characterization, species differentiation and detection of Fasciola spp. by molecular approaches", Parasit Vectors, 4, pp.101~ (2011)
- Hayashi K, "Molecular phylogenetic analysis of Fasciola flukes from eastern India", Parasitol Int, 64, pp.334~338 (2015)
- Amer S, "Identity of Fasciola spp. in sheep in Egypt", Parasit Vectors, 9, pp.623~ (2016)
- Saijuntha W, "Revealing genetic hybridization and DNA recombination of Fasciola hepatica and Fasciola gigantica in nuclear introns of the hybrid Fasciola flukes", Mol Biochem Parasitol, 223, pp.31~36 (2018)
- Agatsuma T, "Molecular evidence of natural hybridization between Fasciola hepatica and F. gigantica", Parasitol Int, 49, pp.231~238 (2000)
- Huang WY, "Characterisation of Fasciola species from Mainland China by ITS-2 ribosomal DNA sequence", Vet Parasitol, 120, pp.75~83 (2004)
- Le TH, "Human fascioliasis and the presence of hybrid", introgressed forms of Fasciola hepatica and Fasciola gigantica in Vietnam, 2008, pp.38, 725~730
- Nguyen TG, "Genotypic characterization and species identification of Fasciola spp. with implications regarding the isolates infecting goats in Vietnam", Exp Parasitol, 123, pp.354~361 (2009)
- Choe SE, "Genetic analysis of Fasciola isolates from cattle in Korea based on second internal transcribed spacer (ITS-2) sequence of nuclear ribosomal DNA", Parasitol Res, 109, pp.833~839 (2011)
- Nguyen S, "Molecular identification of Fasciola spp. (Digenea: Platyhelminthes)in cattle from Vietnam", Parasite, 19, pp.85~89 (2012)
- Amor N, "Molecular characterization of Fasciola spp. from the endemic area of northern Iran based on nuclear ribosomal DNA sequences", Exp Parasitol, 128, pp.196~204 (2011)
- Amer S, "Identification of Fasciola species isolated from Egypt based on sequence analysis of genomic (ITS1 and ITS2) and mitochondrial (NDI and COI) gene markers", Parasitol Int, 60, pp.5~12 (2011)
- Ichikawa-Seki M, "Nuclear and mitochondrial DNA analysis reveals that hybridization between Fasciola hepatica and Fasciola gigantica occurred in China", Parasitology, 144, pp.206~213 (2017)
- Hayashi K, "Hybrid origin of Asian aspermic Fasciola flukes is confirmed by analyzing two single-copy genes, pepck and pold", J Vet Med Sci, 80, pp.98~102 (2018)
- Periago MV, "First phenotypic description of Fasciola hepatica", Fasciola gigantica intermediate forms from the human endemic area of the Nile Delta, Egypt, 2008, pp.8, 51~58
- Wannasan A, "Identification of Fasciola species based on mitochondrial and nuclear DNA reveals the co-existence of intermediate Fasciola and Fasciola gigantica in Thailand", Exp Parasitol, 146, pp.64~70 (2014)
- Ichikawa M, "Discrimination of the ITS1 types of Fasciola spp. based on a PCR-RFLP method", Parasitol Res, 106, pp.757~761 (2010)
- Ai L, "Rapid identification and differentiation of Fasciola hepatica and Fasciola gigantica by a loop-mediated isothermal amplification (LAMP) assay", Vet Parasitol, 174, pp.228~233 (2010)
- Martínez-Valladares M, "Loop-mediated isothermal amplification(LAMP)assay for the diagnosis of fasciolosis in sheep and its application under field conditions", Parasit Vectors, 9, pp.73~ (2016)
- Itagaki T, "Occurrence of spermic diploid and aspermic triploid forms of Fasciola in Vietnam and their molecular characterization based on nuclear and mitochondrial DNA", Parasitol Int, 58, pp.81~85 (2009)
- Shoriki T, "Novel methods for the molecular discrimination of Fasciola spp. on the basis of nuclear protein-coding genes", Parasitol Int, 65, pp.180~183 (2016)
- Terasaki K, "Abnormal spermatogenesis in the common liver fluke (Fasciola sp.) from Japan and Korea", J Vet Med Sci, 60, pp.1305~1309 (1998)
- Ichikawa M, "Characterization of Fasciola spp. in Myanmar on the basis of spermatogenesis status and nuclear and mitochondrial DNA markers", Parasitol Int, 60, pp.474~479 (2011)
- Chaichanasak P, "Identification of Fasciola flukes in Thailand based on their spermatogenesis and nuclear ribosomal DNA, and their intraspecific relationships based on mitochondrial DNA", Parasitol Int, 61, pp.545~549 (2012)
- Mohanta UK, "Characteristics and molecular phylogeny of Fasciola flukes from Bangladesh, determined based on spermatogenesis and nuclear and mitochondrial DNA analyses", Parasitol Res, 113, pp.2493~2501 (2014)
- Bui TD, "Current status of fasciolosis in Vietnam : an update and perspectives", J Helminthol, 90, pp.511~522 (2016)
- Nguyen NT, "High prevalence of cattle fascioliasis in coastal areas of Thua Thien Hue province, Vietnam", J Vet Med Sci, 79, pp.1035~1042 (2017)
- Kumar S, "MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets", Mol Biol Evol, 33, pp.1870~1874 (2016)
- Itagaki T, "Genetic characterization of parthenogenic Fasciola sp. in Japan on the basis of the sequences of ribosomal and mitochondrial DNA", Parasitology, 131, pp.679~685 (2005)
- Walker SM, "Substantial genetic divergence between morphologically indistinguishable populations of Fasciola suggests the possibility of cryptic speciation", Int J Parasitol, 42, pp.1193~1199 (2012)
- King KC, "Hybridization in Parasites: Consequences for Adaptive Evolution, Pathogenesis, and Public Health in a Changing World", PLoS Pathog, 11, pp.e1005098~ (2015)
- Shoriki T, "Molecular phylogenetic identification of Fasciola flukes in Nepal", Parasitol Int, 63, pp.758~762 (2014)
- Dar Y, "Molecular identification of Fasciola spp. (Digenea: Fasciolidae) in Egypt", Parasite, 19, pp.177~182 (2012)
- Hayashi K, "Molecular characterization and phylogenetic analysis of Fasciola gigantica from western Java, Indonesia", Parasitol Int, 65, pp.424~427 (2016)
- Le TH, "Complete DNA sequence and gene organization of the mitochondrial genome of the liverfluke, Fasciola hepatica L. (Platyhelminthes; Trematoda)", Parasitology, 123, pp.609~621 (2001)
