일반혈액검사와 C-Reactive Protein 동시 분석기 LC-175CRPTM의 평가
Performance Evaluation of the LC-175CRPTM Analyzer for Determination of CompleteBlood Cell Count and Quantitative C-Reactive Protein
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배경 : 일반혈액검사와 CRP 정량 검사를 동시에 시행하는 LC- 175CRPTM (Horiba Ltd., Kyoto, Japan)의 분석 능력을 평가하고, 백혈구수와 CRP 동시 측정의 임상적 유용성을 평가하고자하였다. 방법 : 일반혈액검사 및 CRP 정량 검사가 의뢰된 환자 검체와 정도관리물질을 이용하여 기기의 직선성, 정밀도과 상관성을 평가 하였다. 감염성 또는 염증성 질환으로 내원한 환자들 중 일반혈액 검사와 CRP 정량 검사가 시행된 114명의 환자를 선정하여 백혈 구수와 CRP 동시 측정의 유용성을 분석하였다. 결과 : 직선성은 모든 항목에서 결정계수(R2)가 0.98 이상( P< 0.001)이었고, 정밀도는 평가한 모든 항목에 대하여 8% 이하의 총 정밀도 변이계수(total CV)와 검사내정밀도 변이계수(within-run CV)를 보였다. 상관성은 단구 백분율(r=0.35, P=0.02)을 제외한 모든 평가 항목에서 상관계수(r)가 모두 0.94 이상( P<0.001)이었다. 백혈구수와 CRP 둘 중 하나만 이상 소견을 보인 감염/염증성 질환 환자 55명 중 CRP만 증가된 환자가 48명(87.3%)이었다. 결론 : 소형 장비인 LC-175CRP는 직선성, 정밀도, 기존 장비와의 상관성이 모두 우수할 뿐만 아니라, 조작이 쉽고 일반혈액검사와 CRP 정량 결과를 동시에 신속하게 얻을 수 있어 응급실이나 소규모 병의원에서 실용적으로 사용될 수 있는 장비로 생각된다.
Abstract
Background : We evaluated the analytical performance of LC-175CRPTM (Horiba Ltd., Kyoto, Japan), an automated blood cell counter and C-reactive protein (CRP) measuring instrument, and its clinical usefulness. Methods : LC-175CRP was evaluated for linearity, precision, and comparison using patient specimens and quality control material. Usefulness of simultaneous measurement of WBC count and CRP was analyzed using results from 114 patients with infectious or inflammatory diseases. Results : LC-175CRP showed a good linearity (R2≥0.98, P<0.001) and within-run and total-run CVs within 8% for all items analysed. Good correlations were found for all analyzed parameters (r≥ 0.94, P<0.001) except monocyte proportion (r=0.35, P=0.02). Among the 55 patients who showed abnormal results of either WBC or CRP, 48 patients (87.3%) showed CRP elevation alone. Conclusions : LC-175CRP is a small instrument and easy to operate, offers simultaneous, rapid measurement of blood cell count and CRP, and shows an excellent performance. It can be very useful at the emergency room or physician’s office for patients suspected to have acute infections or inflammations.
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