Parasite Infiltration and Apoptosis in Spleen upon Toxoplasma gondii Infection
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Toxoplasma gondii infection induces parasite infiltration and apoptosis in the spleen. However, dose-dependent parasite infiltration, apoptosis, body weight alternations and survival in mice remain largely unknown. In this study, mice were intraperitoneally infected with 10, 30 or 100 tachyzoites of T. gondii, respectively. Parasite infiltration and apoptosis in the spleen were analyzed on days 3, 7, and 9 post-infection by immunohistochemistry and flow cytometry. Significantly higher levels of T. gondii infiltration and apoptosis in the spleen were found in 30 and 100 tachyzoites infected mice compared to 10 tachyzoites infected mice on days 7 and 9 post-infection. Although 30 and 100 tachyzoites infected mice showed significant body weight loss compared to 10 tachyzoites infected mice, all of the 100, 30, and 10 tachyzoites infected mice died by days 12, 15, and 17, each respectively. Interestingly, T. gondii infiltration in 10 tachyzoites infected mice were limited to capsule area of the spleen on day 9 post-infection. Several areas of parasite infiltrations were found in the 30 tachyzoites infected mice, where noticeable levels of splenic capsule de-adhesion occurred. These results indicated that parasite infiltration and apoptosis in the spleen, as well as body weight loss (survival) are closely correlated with infection dosage. The level of T. gondii infiltration and apoptosis in the spleen and splenic de-adhesion were dependent on the parasite dose.
Abstract
Toxoplasma gondii infection induces parasite infiltration and apoptosis in the spleen. However, dose-dependent parasite infiltration, apoptosis, body weight alternations and survival in mice remain largely unknown. In this study, mice were intraperitoneally infected with 10, 30 or 100 tachyzoites of T. gondii, respectively. Parasite infiltration and apoptosis in the spleen were analyzed on days 3, 7, and 9 post-infection by immunohistochemistry and flow cytometry. Significantly higher levels of T. gondii infiltration and apoptosis in the spleen were found in 30 and 100 tachyzoites infected mice compared to 10 tachyzoites infected mice on days 7 and 9 post-infection. Although 30 and 100 tachyzoites infected mice showed significant body weight loss compared to 10 tachyzoites infected mice, all of the 100, 30, and 10 tachyzoites infected mice died by days 12, 15, and 17, each respectively. Interestingly, T. gondii infiltration in 10 tachyzoites infected mice were limited to capsule area of the spleen on day 9 post-infection. Several areas of parasite infiltrations were found in the 30 tachyzoites infected mice, where noticeable levels of splenic capsule de-adhesion occurred. These results indicated that parasite infiltration and apoptosis in the spleen, as well as body weight loss (survival) are closely correlated with infection dosage. The level of T. gondii infiltration and apoptosis in the spleen and splenic de-adhesion were dependent on the parasite dose.
참고문헌 (21)
- Montoya JG, "Toxoplasmosis", Lancet, 363, pp.1965~1976 (2004)
- Tenter AM, "Toxoplasma gondii : from animals to humans", Int J Parasitol, 30, pp.1217~1258 (2000)
- Kim K, "Toxoplasma gondii : the model apicomplexan", Int J Parasitol, 34, pp.423~432 (2004)
- Moncada PA, "Toxoplasmosis in the fetus and newborn : an update on prevalence, diagnosis and treatment", Expert Rev Anti Infect Ther, 10, pp.815~828 (2012)
- Wang S, "Protective immunity against acute toxoplasmosis in BALB", c mice induced by a DNA vaccine encoding Toxoplasma gondii 10 kDa excretory–secretory antigen(TgESA10), 2015, pp.214, 40~48
- Wang S, "Protective immunity against acute toxoplasmosis in BALB", c mice induced by a DNA vaccine encoding Toxoplasma gondii elongation factor 1-alpha, 2015, pp.15, 448~
- Chen J, "DNA immunization with eukaryotic initiation factor-2α of Toxoplasma gondii induces protective immunity against acute and chronic toxoplasmosis in mice", Vaccine, 31, pp.6225~6231 (2013)
- Guo J, "High immunogenicity of virus-like-particle vaccine containing multiple antigenic epitopes of Toxoplasma gondii against acute and chronic toxoplasmosis in mice", Front Immunol, 10, pp.592~ (2019)
- Li ZY, "Synergy of mIL-21 and mIL-15 in enhancing DNA vaccine efficacy against acute and chronic Toxoplasma gondii infection in mice", Vaccine, 32, pp.3058~3065 (2014)
- Lee SH, "Protective immunity induced by incorporating multiple antigenic proteins of Toxoplasma gondii into influenza virus-like particles", Front Immunol, 9, pp.3073~ (2018)
- Lee SH, "Virus-like particle vaccines expressing Toxoplasma gondii rhoptry protein 18 and microneme protein 8 provide enhanced protection", Vaccine, 36, pp.5692~5700 (2018)
- Lee SH, "Protection induced by virus-like particles containing Toxoplasma gondii microneme protein 8 against highly virulent RH strain of Toxoplasma gondii infection", PLoS One, 12, pp.e0175644~ (2017)
- Saraf P, "On the determination of Toxoplasma gondii virulence in mice", Exp Parasitol, 174, pp.25~30 (2017)
- Unno A, "Toxoplasma gondii tachyzoite-infected peripheral blood mononuclear cells are enriched in mouse lungs and liver", Exp Parasitol, 134, pp.160~164 (2013)
- Montazeri M, "In vitro and in vivo evaluation of kojic acid against Toxoplasma gondii in experimental models of acute toxoplasmosis", Exp Parasitol, 200, pp.7~12 (2019)
- Mordue DG, "Acute toxoplasmosis leads to lethal overproduction of Th1 cytokines", J Immunol, 167, pp.4574~4584 (2001)
- Elmore S, "Apoptosis : a review of programmed cell death", Toxicol Pathol, 35, pp.495~516 (2007)
- Joyce BR, "Phosphorylation of eukaryotic initiation factor-2{alpha} promotes the extracellular survival of obligate intracellular parasite Toxoplasma gondii", Proc Natl Acad Sci USA, 107, pp.17200~17205 (2010)
- Chen J, "Protective immunity induced by a DNA vaccine expressing eIF4A of Toxoplasma gondii against acute toxoplasmosis in mice", Vaccine, 31, pp.1734~1739 (2013)
- Bastos da Silva I, "Genotyping of Toxoplasma gondii : DNA extraction from formalin-fixed paraffin-embedded autopsy tissues from AIDS patients who died by severe disseminated toxoplasmosis", Exp Parasitol, 165, pp.16~21 (2016)
- Ramos-Vara JA, "When tissue antigens and antibodies get along : revisiting the technical aspects of immunohistochemistry—the red, brown, and blue technique", Vet Pathol, 51, pp.42~87 (2014)