Monitoring Insecticide Resistance and Target Site Mutations of L1014 Kdr And G119 Ace Alleles in Five Mosquito Populations in Korea
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Mosquitoes are globally distributed and important vectors for the transmission of many human diseases. Mos- quito control is a difficult task and the cost of preventing mosquito-borne diseases is much lower than that for curing the associated diseases. Thus, chemical control remains the most effective tool for mosquito. Due to the long-term intensive use of insecticides to control mosquito vectors, resistance to most chemical insecticides has been reported. This study aimed to investigate the relationship between insecticide resistance and target site mutation of L1014 kdr and G119 ace alleles in 5 species/species group of mosquitoes (Aedes vexans, Ae. albopictus, Anopheles spp., Culex pipiens complex, and Cx. tritaeniorhynchus) obtained from 6 collection sites. For Anopheles spp., the proportion of mosquitoes with mutat- ed alleles in L1014 was 88.4%, homozygous resistant genotypes were observed in 46.7%, and heterozygous resistant genotypes were observed in 41.8%. For the Cx. pipiens complex and Cx. tritaeniorhynchus species, homozygous resis- tant genotypes were found in 25.9% and 9.8%, respectively. However, target site mutation of L1014 in the Ae. vexans nipponii and Ae. albopictus species was not observed. Anopheles spp., Cx. pipiens complex, and Cx. tritaeniorhynchus mosquitoes were resistant to deltamethrin and chlorpyriphos, whereas Ae. vexans nipponii and Ae. albopictus were clear- ly susceptible. We also found a correlation between the resistance phenotype and the presence of the L1014 kdr and G119 ace mutations only in the Anopheles spp. population. In this study, we suggest that insecticide resistance poses a growing threat and resistance management must be integrated into all mosquito control programs.
Abstract
Mosquitoes are globally distributed and important vectors for the transmission of many human diseases. Mos- quito control is a difficult task and the cost of preventing mosquito-borne diseases is much lower than that for curing the associated diseases. Thus, chemical control remains the most effective tool for mosquito. Due to the long-term intensive use of insecticides to control mosquito vectors, resistance to most chemical insecticides has been reported. This study aimed to investigate the relationship between insecticide resistance and target site mutation of L1014 kdr and G119 ace alleles in 5 species/species group of mosquitoes (Aedes vexans, Ae. albopictus, Anopheles spp., Culex pipiens complex, and Cx. tritaeniorhynchus) obtained from 6 collection sites. For Anopheles spp., the proportion of mosquitoes with mutat- ed alleles in L1014 was 88.4%, homozygous resistant genotypes were observed in 46.7%, and heterozygous resistant genotypes were observed in 41.8%. For the Cx. pipiens complex and Cx. tritaeniorhynchus species, homozygous resis- tant genotypes were found in 25.9% and 9.8%, respectively. However, target site mutation of L1014 in the Ae. vexans nipponii and Ae. albopictus species was not observed. Anopheles spp., Cx. pipiens complex, and Cx. tritaeniorhynchus mosquitoes were resistant to deltamethrin and chlorpyriphos, whereas Ae. vexans nipponii and Ae. albopictus were clear- ly susceptible. We also found a correlation between the resistance phenotype and the presence of the L1014 kdr and G119 ace mutations only in the Anopheles spp. population. In this study, we suggest that insecticide resistance poses a growing threat and resistance management must be integrated into all mosquito control programs.
참고문헌 (40)
- "2019 Abnormal Weather Report"[URL]
- 염준섭, "국내 모기매개 질환의 현황과 전망", 대한의사협회지, 60(6), pp.468~474 (2017), 대한의사협회[DOI]
- 박영일, "Epidemiological Characteristics of Re-emerging Vivax Malaria in the Republic of Korea (1993-2017)", Parasites, Hosts and Diseases, 56(6), pp.531~543 (2018), 대한기생충학ㆍ열대의학회
- Epidemiology and Management of Vaccine Preventable Diseases, "Korea Centers for Disease Control and Prevention", Korea Centers for Disease Control and Prevention, ~ (2017)
- Kharkongor M, "Effects of the insecticide chlorpyrifos, on hatching, mortality and morphology of Duttaphrynus melanostictus embryos", Chemosphere, 210, pp.917~921 (2018)
- Lu Z, "Understanding the bioavailability of pyrethroids in the aquatic environment using chemical approaches", Environ Int, 129, pp.194~207 (2019)
- Hemingway J, "An overview of insecticide resistance", Science, 298, pp.96~97 (2002)
- Ketterman AJ, "Insect glutathione transferases", Drug Metab Rev, 43, pp.253~265 (2011)
- Zaim M, "Alternative insecticides : an urgent need", Trends Parasitol, 18, pp.161~163 (2002)
- Liu N, "Insecticide resistance in mosquitoes : impact, mechanisms, and research directions", Ann Rev Entomol, 60, pp.537~559 (2015)
- Main BJ, "Genetic variation associated with increased insecticide resistance in the malaria mosquito, Anopheles coluzzii", Parasit Vectors, 11, pp.225~ (2018)
- Dong K, "A single amino acid change in the para sodium channel protein is associated with knockdown-resistance(kdr)to pyrethroids insecticides in German cockroach", Insect Biochem Mol Biol, 27, pp.93~100 (1997)
- Busvine JR, "Mechanism of resistance to insecticide in houseflies", Nature, 168, pp.193~195 (1951)
- Soderlund DM, "The molecular biology of knockdown resistance to pyrethroids insecticides", Insec Biochem Mol Biol, 33, pp.563~577 (2003)
- Weill M, "Comparative genomics : Insecticide resistance in mosquito vectors", Nature, 423, pp.136~137 (2003)
- Weill M, "The unique mutation in ace-1 giving high insecticide resistance is easily detectable in mosquito vectors", Insect Mol Biol, 13, pp.1~7 (2004)
- Lanzaro GC, "Complexities in the genetic structure of Anopheles gambiae populations in west Africa as revealed by microsatellite DNA analysis", Proc Natl Acad Sci USA, 95, pp.14260~14265 (1998)
- Schmidt JM, "Copy number variation and transposable elements feature in recent, ongoing adaptation at the Cyp6g1 locus", PLoS Genet, 6, pp.e1000998~ (2010)
- Tanaka K, "A Revision of the Adult and Larval Mosquitoes of Japan (including the Ryukyu Archipelago and the Ogasawara Islands) and Korea (Diptera: Culicidae)", American Entomological Institute, ~ (1979)
- Lee KW, "A Revision of the Illustrated Taxonomic Keys to Genera and Species of Female Mosquitoes of Korea", Department of the Army, 5th Medical Detachment, 168th Medical Battalion, 18th Medical Command, 40~ (1998)
- Jegal S, "Three-year surveillance of culicine mosquiotes(Diptera : Culicidae)for flavivirus infections in Incheon metropolitan city and Hwaseong-si of Gyeonggi-do province, Republic of Korea", Acta Trop, 202, pp.105258~ (2020)
- 박영일, "Flaviviral Disease Mosquito Vector Surveillance in Incheon Metropolitan City and the Hwaseong Area, Gyeonggi-Do, Republic of Korea, in 2015", Entomological Research, 50(1), pp.3~13 (2020), 한국곤충학회
- "Test Procedures for Insecticide Resistance Monitoring in Malaria Vector Mosquitoes"[URL]
- Desmond H. Foley, "Mosquito Species Composition and Plasmodium vivax Infection Rates on Baengnyeong-do (Island), Republic of Korea", Parasites, Hosts and Diseases, 49(3), pp.313~316 (2011), 대한기생충학ㆍ열대의학회
- Zaim M, "Safety of pyrethroid-treated mosquito nets", Med Vet Entomol, 14, pp.1~5 (2000)
- World Wildlife Fund, "Hazards and Exposures Associated with DDT and Synthetic Pyrethroids used for Vector Control", World Wildlife Fund, ~ (1999)
- Martinez-Torres D, "Voltage-dependent Na+ channels in pyrethroid-resistant Culex pipiens L. mosquitoes", Pest Manag Sci, 55, pp.1012~1020 (1999)
- Xu Q, "Sodium channel genes and their differential genotypes at the F-to-F kdr locus in the mosquito Culex quinquefasciatus", Biochem Biophys Res Comm, 40, pp.645~649 (2011)
- Wang ZM, "Detection and widespread distribution of sodium channel alleles characteristic of insecticide resistance in Culex pipiens complex mosquitoes in China", Med Vet Entomol, 26, pp.228~232 (2012)
- Diabate A, "The spread of the Leu-Phe kdr mutation through Anopheles gambiae complex in Burkina Faso : genetic introgression and de novo phenomena", Tropical Med Int Health, 9, pp.1267~1273 (2004)
- Scott JG, "Pyrethroid resistance in Culex pipiens mosquitoes", Pest Biochem Physiol, 120, pp.68~76 (2015)
- Brooke BD, "kdr: can a single mutation produce an entire insecticide resistance phenotype?", Trans R Soc Trop Med Hyg, 102, pp.524~525 (2008)
- Rinkevich FD, "Diversity and convergence of sodium channel mutations involved in resistance to pyrethroids", Pestic Biochem Physiol, 106, pp.93~100 (2013)
- Weill M, "Comparative genomics : insecticide resistance in mosquito vectors", Nature, 423, pp.136~137 (2003)
- Sinegre G, "Acquisition progressive de la résistance a chlorpyiphos chez les larves de Culex pipiens(L)dans le midi la France", Parasitologia, 19, pp.1~2 (1977)
- Tmimi FZ, "Insecticide resistance and target site mutations(G119S ace-1 and L1014F kdr)of Culex pipiens in Morocco", Parasit Vectors, 11, pp.51~ (2018)
- Chavasse DC, "Chemical Methods for the Control of Vectors and Pests of Public Health Importance, Vol. 2", pp.129~ (1997), World Health Organization
- 여인석, "U.S. Military Administration’s Malaria Control Activities (1945-1948)", 의사학, 24(1), pp.35~65 (2015), 대한의사학회[DOI]
- Yadouléton A, "Insecticide resistance status in Culex quinquefasciatus in Benin", Parasit Vectors, 8, pp.17~ (2015)
- Antonio-Nkondjio C, "Investigation of bendiocarb resistance in Anopheles gambiae populations from the city of Yaoundé, Cameroon", Malar J, 15, pp.424~ (2016)
