본문 바로가기
KERD
논문KCI 등재

Prevalence and Multilocus Genotyping of Giardia lamblia in Cattle in Jiangxi Province, China: Novel Assemblage E Subtypes Identified

0

조회

0

다운로드

0

인용

0

참고문헌

키워드

초록

Giardia lamblia is a common enteric pathogen associated with diarrheal diseases. There are some reports of G. lamblia infection among different breeds of cattle in recent years worldwide. However, it is yet to know whether cattle in Jiangxi province, southeastern China is infected with G. lamblia. The objectives of the present study were to investigate the prevalence and examine the multilocus genotypes of G. lamblia in cattle in Jiangxi province. A total of 556 fecal sam- ples were collected from 3 cattle breeds (dairy cattle, beef cattle, and buffalo) in Jiangxi province, and the prevalence and genotypes of G. lamblia were determined by the nested PCR amplification of the beta-giardin (bg) gene. A total of 52 samples (9.2%) were positive for G. lamblia. The highest prevalence of G. lamblia was detected in dairy cattle (20.0%), followed by that in beef cattle (6.4%), and meat buffalo (0.9%). Multilocus sequence typing of G. lamblia was performed based on sequences of the bg, triose phosphate isomerase and glutamate dehydrogenase loci, and 22, 42, and 52 sam- ples were amplifiable, respectively, forming 15 MLGs. Moreover, one mixed G. lamblia infection (assemblages A and E) was found in the present study. Altogether, 6 novel assemblage E subtypes (E41*-E46*) were identified for the first time. These results not only provided baseline data for the control of G. lamblia infection in cattle in this southeastern province of China, but also enriched the molecular epidemiological data and genetic diversity of G. lamblia in cattle.

Abstract

Giardia lamblia is a common enteric pathogen associated with diarrheal diseases. There are some reports of G. lamblia infection among different breeds of cattle in recent years worldwide. However, it is yet to know whether cattle in Jiangxi province, southeastern China is infected with G. lamblia. The objectives of the present study were to investigate the prevalence and examine the multilocus genotypes of G. lamblia in cattle in Jiangxi province. A total of 556 fecal sam- ples were collected from 3 cattle breeds (dairy cattle, beef cattle, and buffalo) in Jiangxi province, and the prevalence and genotypes of G. lamblia were determined by the nested PCR amplification of the beta-giardin (bg) gene. A total of 52 samples (9.2%) were positive for G. lamblia. The highest prevalence of G. lamblia was detected in dairy cattle (20.0%), followed by that in beef cattle (6.4%), and meat buffalo (0.9%). Multilocus sequence typing of G. lamblia was performed based on sequences of the bg, triose phosphate isomerase and glutamate dehydrogenase loci, and 22, 42, and 52 sam- ples were amplifiable, respectively, forming 15 MLGs. Moreover, one mixed G. lamblia infection (assemblages A and E) was found in the present study. Altogether, 6 novel assemblage E subtypes (E41*-E46*) were identified for the first time. These results not only provided baseline data for the control of G. lamblia infection in cattle in this southeastern province of China, but also enriched the molecular epidemiological data and genetic diversity of G. lamblia in cattle.

참고문헌 (46)

  1. Feng YY, "Zoonotic potential and molecular epidemiology of Giardia species and Giardiasis", Clin Microbiol Rev, 24, pp.110~140 (2011)[DOI]
  2. Bartelt LA, "Advances in understanding Giardia : determinants and mechanisms of chronic sequelae", F1000Prime Rep, 7, pp.62~ (2015)[DOI]
  3. Ryan U, "Zoonotic potential of Giardia", Int J Parasitol, 43, pp.110~140 (2013)[DOI]
  4. Thompson RCA, "Molecular epidemiology of Giardia and Cryptosporidium infections", Infect Genet Evol, 40, pp.315~323 (2016)[DOI]
  5. Abdel-Moein KA, "The zoonotic potential of Giardia intestinalis assemblage E in rural settings", Parasitol Res, 115, pp.3197~3202 (2016)[DOI]
  6. Hogan JN, "Giardia in mountain gorillas(Gorilla beringei beringei), forest buffalo(Syncerus caffer), and domestic cattle in Volcanoes National Park, Rwanda", J Wildl Dis, 50, pp.21~30 (2014)[DOI]
  7. Feng Y, "Prevalence and genotypic identification of Cryptosporidium spp., Giardia duodenalis and Enterocytozoon bieneusi in preweaned dairy calves in Guangdong, China", Parasit Vectors, 12, pp.41~ (2019)[DOI]
  8. Wang R, "Infection patterns, clinical significance, and genetic characteristics of Enterocytozoon bieneusi and Giardia duodenalis in dairy cattle in Jiangsu, China", Parasitol Res, 118, pp.3053~3060 (2019)[DOI]
  9. Bartley PM, "Detection of potentially human infectious assemblages of Giardia duodenalis in fecal samples from beef and dairy cattle in Scotland", Parasitology, 146, pp.1123~1130 (2019)[DOI]
  10. Lee YJ, "Identification of zoonotic Giardia duodenalis in Korean native calves with normal feces", Parasitol Res, 117, pp.1969~1973 (2018)[DOI]
  11. Wang X, "High genetic diversity of Giardia duodenalis assemblage E in pre-weaned dairy calves in Shanghai, China, revealed by multilocus genotyping", Parasitol Res, 116, pp.2101~2110 (2017)[DOI]
  12. Yu F, "Molecular characterization of three intestinal protozoans in hospitalized children with different disease backgrounds in Zhengzhou, central China", Parasit Vectors, 12, pp.543~ (2019)[DOI]
  13. Wang T, "First survey of Cryptosporidium, Giardia and Enterocytozoon in diarrhoeic children from Wuhan, China", Infect Genet Evol, 51, pp.127~131 (2017)[DOI]
  14. Fantinatti M, "Identification of Giardia lamblia assemblage E in human points to a new anthropozoonotic cycle", J Infect Dis, 214, pp.1256~1259 (2016)[DOI]
  15. Zahedi A, "Molecular typing of Giardia duodenalis in humans in Queensland-first report of assemblage E", Parasitology, 144, pp.1154~1161 (2017)[DOI]
  16. Di Piazza F, "A study on occupational exposure of Sicilian farmers to Giardia and Cryptosporidium", J Prev Med Hyg, 54, pp.212~217 (2013)
  17. Mahato MK, "Prevalence and risk factors associated with Giardia duodenalis infection in dairy cattle of Chitwan, Nepal", J Parasit Dis, 42, pp.122~126 (2018)[DOI]
  18. Wang H, "Multilocus genotyping of Giardia duodenalis in dairy cattle in Henan, China", PLoS One, 9, pp.e100453~ (2014)[DOI]
  19. Li J, "Giardia duodenalis Infections in Humans and other animals in China", Front Microbiol, 8, pp.2004~ (2017)[DOI]
  20. Lalle M, "Genetic heterogeneity at the beta-giardin locus among human and animal isolates of Giardia duodenalis and identification of potentially zoonotic subgenotypes", Int J Parasitol, 35, pp.207~213 (2005)[DOI]
  21. Azcona-Gutiérrez JM, "Molecular diversity and frequency of the diarrheagenic enteric protozoan Giardia duodenalis and Cryptosporidium spp. in a hospital setting in northern Spain", PLoS One, 12, pp.e0178575~ (2017)[DOI]
  22. Thompson JD, "The CLUSTAL_X windows interface : flexible strategies for multiple sequence alignment aided by quality analysis tools", Nucleic Acids Res, 25, pp.4876~4882 (1997)[DOI]
  23. Saitou N, "The neighbor-joining method : a new method for reconstructing phylogenetic trees", Mol Biol Evol, 4, pp.406~425 (1987)[DOI]
  24. Burland TG, "DNASTAR's Lasergene sequence analysis software", Methods Mol Biol, 132, pp.71~91 (2000)[DOI]
  25. Zhang XX, "Prevalence, risk factors and multilocus genotyping of Giardia intestinalis in dairy cattle, Northwest China", J Eukaryot Microbiol, 63, pp.498~504 (2016)[DOI]
  26. Mahato MK, "Prevalence and risk factors associated with Giardia duodenalis infection in dairy cattle of Chitwan. Nepal", J Parasit Dis, 42, pp.122~126 (2018)[DOI]
  27. Maddox-Hyttel C, "Cryptosporidium and Giardia in different age groups of Danish cattle and pigs—occurrence and management associated risk factors", Vet Parasitol, 141, pp.48~59 (2006)[DOI]
  28. Cui Z, "Genetic characteristics and geographic segregation of Giardia duodenalis in dairy cattle from Guangdong Province, southern China", Infect Genet Evol, 66, pp.95~100 (2018)[DOI]
  29. Wang G, "Prevalence and molecular characterization of Cryptosporidium spp. and Giardia duodenalis in 1–2-month-old highland yaks in Qinghai Province, China", Parasitol Res, 117, pp.1793~1800 (2018)[DOI]
  30. Kiani-Salmi N, "Molecular typing of Giardia duodenalis in cattle, sheep and goats in an arid area of central Iran", Infect Genet Evol, 75, pp.104021~ (2019)[DOI]
  31. Inpankaew T, "Prevalence and genotype of Giardia duodenalis in dairy cattle from northern and northeastern part of Thailand", Acta Parasitol, 60, pp.459~461 (2015)[DOI]
  32. Malekifard F, "Molecular detection and identification of Giardia duodenalis in cattle of Urmia, northwest of Iran", Vet Res Forum, 9, pp.81~85 (2018)
  33. Fan Y, "Molecular investigation of Cryptosporidium and Giardia in pre-and post-weaned calves in Hubei Province, China", Parasit Vectors, 10, pp.519~ (2017)[DOI]
  34. Zhong Z, "Occurrence and genotyping of Giardia duodenalis and Cryptosporidium in pre-weaned dairy calves in central Sichuan province, China", Parasite, 25, pp.45~ (2018)[DOI]
  35. Naguib D, "Age patterns of Cryptosporidium species and Giardia duodenalis in dairy calves in Egypt", Parasitol Int, 67, pp.736~741 (2018)[DOI]
  36. Lee YJ, "Identification of zoonotic Giardia duodenalis in Korean native calves with normal feces", Parasitol Res, 117, pp.1969~1973 (2018)[DOI]
  37. Wegayehu T, "Multilocus genotyping of Giardia duodenalis isolates from calves in Oromia Special Zone, Central Ethiopia", Infect Genet Evol, 43, pp.281~288 (2016)[DOI]
  38. Nguyen ST, "Prevalence and first genotyping of Giardia duodenalis in beef calves in Vietnam", Trop Anim Health Prod, 48, pp.837~841 (2016)[DOI]
  39. Zhang HJ, "First report of Giardia duodenalis genotypes in Zangxiang pigs from China", Parasitol Res, 118, pp.2305~2310 (2019)[DOI]
  40. Chen D, "Occurrence and multilocus genotyping of Giardia duodenalis in black-boned sheep and goats in southwestern China", Parasit Vectors, 12, pp.102~ (2019)[DOI]
  41. Minetti C, "Occurrence and diversity of Giardia duodenalis assemblages in livestock in the UK", Transbound Emerg Dis, 61, pp.60~67 (2014)[DOI]
  42. Xie SC, "Occurrence and Multilocus Genotyping of Giardia duodenalis in Yunnan Black Goats in China", Biomed Res Int, 2018, pp.4601737~ (2018)[DOI]
  43. Wang SS, "Prevalence and multilocus genotyping of Giardia duodenalis in pigs of Shaanxi Province, northwestern China", Parasit Vectors, 10, pp.490~ (2017)[DOI]
  44. Roegner AF, "Giardia infection and Trypanosoma cruzi exposure in dogs in the Bosawás biosphere reserve, Nicaragua", Ecohealth, 16, pp.512~522 (2019)[DOI]
  45. Huang J, "Prevalence and molecular characterization of Cryptosporidium spp. and Giardia duodenalis in deer in Henan and Jilin, China", Parasit Vectors, 11, pp.239~ (2018)[DOI]
  46. Cacciò SM, "Multilocus genotyping of Giardia duodenalis reveals striking differences between assemblages A and B", Int J Parasitol, 38, pp.1523~1531 (2008)[DOI]

관련 논문AI추천

Genotyping of Giardia duodenalis isolates from dogs in Guangdong, China based on multi-locus sequence.Guochao Zheng, Guoqing Li, Liping Tan, Muhamd Alsarakibi, Qin Luo, Wei Hu, Yuanjia Liu대한기생충학ㆍ열대의학회 · Parasites, Hosts and Diseases2014 · 제52권 제3호Occurrence and Molecular Identification of Giardia duodenalis from Stray Cats in Guangzhou, Southern ChinaGuochao Zheng, Guoqing Li, Wei Hu,, Yuanjia Liu, Qin Luo대한기생충학ㆍ열대의학회 · Parasites, Hosts and Diseases2015 · 제53권 제1호Prevalence and Subtypes of Blastocystis in Alpacas, Vicugna pacos in Shanxi Province, ChinaQing Liu, Shi-Chen Xie, Tao-Shan Li, Wen-Wei Gao, Xiao-Dong Li, Xing-Quan Zhu, Ye-Ting Ma, Yuan-Yuan Ma대한기생충학ㆍ열대의학회 · Parasites, Hosts and Diseases2020 · 제58권 제2호Molecular Detection of Giardia intestinalis from Stray Dogs in Animal Shelters of Gyeongsangbuk-do (Province) and Daejeon, Korea김석, 서경원, 송근호, Hyung-Jin Park, Jin-Cheol Shin, REYESALISHA WEHDNESDAY, Sang-Hun Kim대한기생충학ㆍ열대의학회 · Parasites, Hosts and Diseases2015 · 제53권 제4호Cryptosporidium spp., Giardia intestinalis, and Enterocytozoon bieneusi in Captive Non-Human Primates in Qinling MountainsGe-Ru Tian, Guang-Hui Zhao, Hai-Yan Wang, Jun-Feng Shao, Meng Qi, Rong-Jun Wang, San-Ke Yu, Shuai-Zhi Du, Yan-Qin Fang대한기생충학ㆍ열대의학회 · Parasites, Hosts and Diseases2015 · 제53권 제4호Molecular Detection and Subtyping of Blastocystis in Korean Pigs곽동미, 이만희, 이해승, 한지은, 황미혜, Byeong Yeal Jung, Oh Deog Kwon, Seunghyun Paik, Tae-Hwan Kim대한기생충학ㆍ열대의학회 · Parasites, Hosts and Diseases2019 · 제57권 제5호

이 간행물의 다른 논문 (14건)