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IMP-1과 OXA -lactamase를 생성하는 Carbapenem 내성 Acinetobacter baumanni 의 확산

Dissemination of IMP-1 and OXA Type -Lactamase in Carbapenem-resistantAcinetobacter baumannii

신경섭|박종우|신소연|김종완|김연숙|김지명|송정훈|구선회|성지연|권계철|고지선
대한진단검사의학회 (Korean Society For Laboratory Medicine)|2008.02|Annals of Laboratory Medicine|제28권 제1호|pp. 16-23

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참고문헌

키워드

MBLA. baumanniiOXA-2
주제분류병리학

초록

Background : Acinetobacter baumannii is an aerobic, gram-negative, glucose-nonfermenting bacterium, which has emerged as a serious opportunistic pathogen. In recent years, the increasing instance of carbapenem-resistant A. baumannii producing metallo- -lactamases (MBLs) or OXAtype -lactamases is causing a serious clinical problem. In this study, we investigated the prevalence of Ambler class A, B, and D -lactamases and their extended-spectrum derivatives in carbapenem- resistant A. baumannii isolates. Methods : A total of 31 consecutive, non-duplicate, carbapenem-resistant A. baumannii were isolated from three university hospitals in the Chungcheong province of Korea. The modified Hodge and inhibitor-potentiated disk diffusion tests were conducted for the screening of carbapenemase and MBL production, respectively. PCR and DNA sequencing were performed for the detection of -lactamase genes. We also employed the enterobacterial repetitive intergenic consensus (ERIC)- PCR method for the epidemiologic study. Results : Twenty-three of 31 isolates harbored blaOXA-2 (51.6%), blaOXA-23 (22.6%), blaIMP-1 (48.4%), and blaVIM-2 (3.2%). All of the OXA-2-producing strains also evidenced MBLs. The strains that harbored blaOXA-23 were isolated only in hospital C, and only in a limited fashion. The ERIC-PCR pattern of the five OXA-23 strains indicated that the isolates were closely related in terms of clonality. The six strains producing IMP-1 isolated from hospital A were confirmed to be identical strains. Conclusions : A. baumannii strains harboring IMP-1 or OXA-type -lactamases are currently widely distributed throughout the Chungcheong province of Korea. The most notable finding in this study was that a blaOXA-2-producing A. baumannii harboring MBL, which has not been previously reported, can also lead to outbreaks. (Korean J Lab Med 2008;28:16-23)

Abstract

Background : Acinetobacter baumannii is an aerobic, gram-negative, glucose-nonfermenting bacterium, which has emerged as a serious opportunistic pathogen. In recent years, the increasing instance of carbapenem-resistant A. baumannii producing metallo- -lactamases (MBLs) or OXAtype -lactamases is causing a serious clinical problem. In this study, we investigated the prevalence of Ambler class A, B, and D -lactamases and their extended-spectrum derivatives in carbapenem- resistant A. baumannii isolates. Methods : A total of 31 consecutive, non-duplicate, carbapenem-resistant A. baumannii were isolated from three university hospitals in the Chungcheong province of Korea. The modified Hodge and inhibitor-potentiated disk diffusion tests were conducted for the screening of carbapenemase and MBL production, respectively. PCR and DNA sequencing were performed for the detection of -lactamase genes. We also employed the enterobacterial repetitive intergenic consensus (ERIC)- PCR method for the epidemiologic study. Results : Twenty-three of 31 isolates harbored blaOXA-2 (51.6%), blaOXA-23 (22.6%), blaIMP-1 (48.4%), and blaVIM-2 (3.2%). All of the OXA-2-producing strains also evidenced MBLs. The strains that harbored blaOXA-23 were isolated only in hospital C, and only in a limited fashion. The ERIC-PCR pattern of the five OXA-23 strains indicated that the isolates were closely related in terms of clonality. The six strains producing IMP-1 isolated from hospital A were confirmed to be identical strains. Conclusions : A. baumannii strains harboring IMP-1 or OXA-type -lactamases are currently widely distributed throughout the Chungcheong province of Korea. The most notable finding in this study was that a blaOXA-2-producing A. baumannii harboring MBL, which has not been previously reported, can also lead to outbreaks. (Korean J Lab Med 2008;28:16-23)

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OXA-23 혹은 IMP-1 β-Lactamase 생성 Acinetobacter baumannii에 의한 감염의 분자 역학박광옥, 배일권, 손한철, 정석훈대한임상미생물학회 · Annals of Clinical Microbiology2005 · 제8권 제2호Molecular and Epidemiological Characterization of Carbapenem-Resistant Acinetobacter baumannii in Non-Tertiary Korean Hospitals김화수, 박선옥, 유재일, 유정식, 이경민, 이영선, 정경태연세대학교의과대학 · Yonsei Medical Journal2013 · 제54권 제1호국내 대학병원에서 분리된 Metallo-β-Lactamase (MBL) 생성 Acinetobacter spp. 분리주의 높은 출현율과 유전형 특징염종화대한임상검사과학회 · 대한임상검사과학회지2019 · 제51권 제4호Characteristics of Acquired β-lactamase Gene in Clinical Isolates of Multidrug-resistant Pseudomonas aeruginosa고지선, 구선회, 권계철, 박종우, 성지연, 송정훈, 신소연, 정선양대한임상미생물학회 · Annals of Clinical Microbiology2008 · 제11권 제2호1·2차 의료기관 임상 검체에서 분리된 Acinetobacter spp.의 항균제 내성과 Metallo-β-Lactamase 생성주 검출김봉수, 유용선, 유재일, 이영선, 이점규대한감염학회 · Infection and Chemotherapy2004 · 제36권 제5호2006년 서울과 경기지역 4개 병원에서 분리된 Carbapenem 내성 Acinetobacter spp.의 내성 기전과 분자역학김창기, 노경호, 염종화, 용동은, 이경원, 이창규, 임채승, 정석훈, 정윤섭, 조윤정대한임상미생물학회 · Annals of Clinical Microbiology2010 · 제13권 제1호

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