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논문KCI 등재

침습 유방암 환자의 HER2 유전자 증폭 검사를 위한 미세침흡인물의 유용성

Availability of Fine Needle Aspirates for the Assessment of HER2 Gene Amplification in Invasive Breast Cancer Patients

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Abstract

Background : FISH and immunohistochemistry (IHC) on formalin-fixed paraffin embedded (FFPE) tissue are currently used in the clinical laboratory to determine HER2 status in invasive breast cancer patients. Since tissue-based methods are relatively time-consuming and have a limitation for standardization of procedure, we evaluated the availability of fine needle aspirates (FNA) for the assessment of HER2 status in invasive breast cancer patients. Methods : FNA were obtained from 51 invasive breast cancer patients and were submitted for the evaluation of HER2 status. After invasive breast cancer components were ascertained by morphological evaluation, HER2 gene amplification was evaluated by FISH. The results of HER2 FISH on FNA cells were compared with those of both FISH and IHC on corresponding FFPE tissues. FISH results were interpreted by American Society of Clinical Oncology (ASCO)/College of American Pathologists (CAP) guidelines issued in 2007. Results : Of 51 FNA specimens, one was excluded due to an insufficient number of cancer cells for tests. Excluding the cases that showed ‘equivocal’ results, 47 (98%) out of 48 cases were concordant between the results of FISH on FNA and FISH on corresponding FFPE tissue (kappa, 0.969), and 43 (93%) out of 46 cases were concordant between the results of FISH on FNA and IHC on corresponding FFPE tissue (kappa, 0.912). Conclusions : An excellent correlation was found between FISH on FNA cells and corresponding FFPE sections. We recommend FNA specimens for more rapid determination of HER2 status by FISH, which will be helpful for patient selection for individualized therapy. (Korean J Lab Med 2008;28:392-9)

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관련 논문AI추천

유방암종의 바늘 생검 조직에 대한 동결절편 검사의 유용성강성수, 김의정, 김혜선, 김희숙, 이성공, 이지현, 전이경, 홍성란대한병리학회 · Journal of Pathology and Translational Medicine2002 · 제36권 제3호유방암에서 HER2/ neu 유전자 정량과 MAGE 검사의 임상적 의의박호용, 서장수, 이난영, 이영하대한진단검사의학회 · Annals of Laboratory Medicine2004 · 제24권 제6호유방암종의 HER-2/neu검색을 위한 형광제자리부합화와 면역조직화학 염색법의 비교김정연, 박경미, 임성직대한병리학회 · Journal of Pathology and Translational Medicine2002 · 제36권 제4호유방암에서 색소원제자리부합화에 의한 HER-2/neu 종양유전자 증폭과 Topoisomerase II-α 의 면역조직화학적 발현권귀영, 김미경, 박언섭, 오형균, 유재형, 이태진대한병리학회 · Journal of Pathology and Translational Medicine2003 · 제37권 제1호유방암에서 HER2 유전자 검사를 위한 자동화 은제자리부합법 검사의 유용성: 형광제자리부합법과 면역조직화학염색과의 비교구미진, 박석주, 배영경, 성우정대한병리학회 · Journal of Pathology and Translational Medicine2010 · 제44권 제1호유방암 환자에서 인간표피성장인자수용체 2의 발현 평가 방법인 면역조직화학염색법과 은제자리부합법의 일치율과 중등도 양성의 비율권순하, 김성용, 백무준, 오미혜, 이득영, 이종은, 이지현, 이현주, 한선욱한국유방암학회 · Journal of Breast Disease2014 · 제2권 제2호

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