본문 바로가기
KERD
논문KCI 등재

In vitro culture of Cryptosporidium muris in a human stomach adenocarcinoma cell line

홍성태|채종일|최민호|박우윤|유재란
대한기생충학ㆍ열대의학회 (The Korean Society For Parasitology and Tropical Medicine)|2004.03|Parasites, Hosts and Diseases|제42권 제1호|pp. 27-34

0

조회

0

다운로드

0

인용

0

참고문헌

주제분류예방의학/직업환경의학

초록

Abstract

We investigated the optimal culture conditions for Cryptosporidium muris in a human stomach adenocarcinoma (AGS) cell line by determining the effects of medium pH and of selected supplements on the development of C. muris. The optimum pH of the culture medium required for the development of C. muris was determined to be 6.6. The number of parasites significantly increased during cultivation for 72 hr (p<0.05) at this level. On the other hand, numbers decreased linearly after 24 hr of incubation at pH 7.5. When cultured in different concentrations of serum, C. muris in media containing 5% FBS induced 4-7 times more parasites than in 1% or 10% serum. Of the six medium supplements examined, only 1 mM pyruvate enhanced the number of C. muris in vitro. Transmission electron microscopic observation showed the developmental stages of C. muris in the cytoplasm of the cells, not in an extracytoplasmic location. The growth of C. muris in AGS cells provides a means of investigating its biological characteristics and of testing its response to therapeutic agents. However, a more optimized culture system is needed for the recovery of oocysts on a large scale in vitro.

참고문헌 (17)

  1. "The effects of reducing conditions and time on in vitro excystation of Cryptosporidium", pp.567~569 (1984)
  2. "Cryptosporidium muris infection in an HIV-infected adult, Kenya", 8, pp.204~206 (2002)
  3. "in vitro cultivation in Madin-Darby canine kidney cells" (1991)
  4. "Attachment of Cryptosporidium parvum sporozoites to MDCK cells in vitro", pp.2208~2213 (1994)
  5. "Complete development and long-term maintenance of Cryptosporidium parvum human and cattle genotypes in cell culture", 31, pp.1048~1055 (2001)
  6. "Successful in vitro cultivation of Cryptosporidium andersoni: evidence for the existence of novel extracellular stages in the life cycle and implications for the classification of Cryptosporidium", 32, pp.1719~1726 (2002)
  7. "Infectivity of Cryptosporidium muris", pp.218~222 (1989)
  8. "Short report: possible Cryptosporidium muris infection in humans", 62, pp.70~72 (2000)
  9. "Simplified methods for obtaining purified oocysts from mice and for growing Cryptosporidium parvum in vitro", 82, pp.757~762 (1996)
  10. "Murine infection model for maintenance and amplification of Cryptosporidium parvum oocysts", 33, pp.1922~1924 (1995)
  11. "Cryptosporidiosis: biology, pathogenesis and disease", 4, pp.1047~1058 (2002)
  12. "Ultrastructure of Cryptosporidium muris", pp.123~132 (1987)
  13. "Effect of select media supplements on motility and development of Eimeria nieschulzi in vitro", pp.329~333 (1992)
  14. "Effects of select medium supplements on in vitro development of Cryptosporidium parvum in HCT-8 cells", 33, pp.371~375 (1995)
  15. "Host cell apoptosis impairs Cryptosporidium parvum development in vitro", 86, pp.922~928 (2000)
  16. "TThe effect of microfilament inhibitor on the Cryptosporidium infection in vitro", 38, pp.257~261 (2000)
  17. "In vitro infection of Cryptosporidium parvum to four different cell lines", 38, pp.59~64 (2000)

관련 논문AI추천

Viability of preserved Cryptosporidium baileyi oocystsChan-GuSURL, Hyeon-CheolKIM, Se-MinKIM대한기생충학ㆍ열대의학회 · Parasites, Hosts and Diseases2003 · 제41권 제4호Genotype and animal infectivity of a human isolate of Cryptosporidium parvum in the Republic of Korea (Brief Communication)국상미, 박순정, 박재환, 용태순, 채종일대한기생충학ㆍ열대의학회 · Parasites, Hosts and Diseases2004 · 제42권 제2호Infection status of pigs with Cryptosporidium parvum (Brief Communication)서민, 유재란대한기생충학ㆍ열대의학회 · Parasites, Hosts and Diseases2004 · 제42권 제1호Role of murine Peyer’s patch lymphocytes against primary and challenge infections withCryptosporidium parvum국상미, 채종일대한기생충학ㆍ열대의학회 · Parasites, Hosts and Diseases2007 · 제45권 제3호Quantitative Evaluation of Infectivity Change of Cryptosporidium parvum after Gamma Irradiation박우윤, 유재란, 이수응, Mikyo Joung, Taekyoung Nam대한기생충학ㆍ열대의학회 · Parasites, Hosts and Diseases2009 · 제47권 제1호Time gap between oocyst shedding and antibody responses in mice infected with Cryptosporidium parvum유재란, 이수응대한기생충학ㆍ열대의학회 · Parasites, Hosts and Diseases2007 · 제45권 제3호

이 간행물의 다른 논문 (6건)

Prevalence of cryptosporidiosis among the villagers and domestic animals in several rural areas of Korea이규재, 유재란, 서민, 손운목, 김동수, 최해연, 허선, 김석일pp. 1-6Bile duct changes in rats reinfected with Clonorchis sinensis홍성태, 임재훈, 정병석, Shunyu Li, 최동일, 이순형pp. 7-17Development of resistance to reinfection by Clonorchis sinensis in ratsHongman Zhang, Shunyu Li, 정병석, 이매정, 홍성태, 최민호, 전동규pp. 19-26Decreasing effect of an anti-Nfa1 polyclonal antibody on the in vitro cytotoxicity of pathogenic Naegleria fowleri정석률, 강수연, 이상철, 송경주, 신호준, 임경일pp. 35-40Clonorchis sinensis metacercarial infection in the pond smelt Hypomesus olidus and the minnow Zacco platypus collected from the Soyang and Daechung Lakes (Brief Communication)김태윤, 신은희, 박지연, 김재립, 박재환, 국상미, Aifen Lin, 채종일, 홍성태pp. 41-44
원문 보기