Development of Indicators to Assess the Stability of Remnant Blood Samples Stored in a Biobank: Experience at One Institution
0
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Background : One of the major concerns with biobanking is the absence of standard operating procedures to eliminate pre-analytical variation arising from sample collection, preparation, and storage. Currently, there is a lack of tools to carry out quality control procedures for stored blood samples. The aim of this study is to assess the quality of stored blood samples in our biobank and to suggest appropriate indicators for their quality control. Methods : The stored blood samples that we tested have been registered into our biobank since 2003. These were transferred to our biobank after carrying out routine requested tests, because the samples would have otherwise been discarded. For the purpose of quality control, we analyzed the concentrations and the integrity of DNA and RNA extracted from the stored samples and tested the levels of several serum proteins; the results were compared with the corresponding pre-storage levels. Results : A total of 19 samples were stored from 2006 to 2009. Of the 22 samples stored between 2003 and 2005, 50% showed complete DNA integrity. However, sufficient RNA integrity was noted in only 1 sample stored as recently as 2009. High blood urea nitrogen levels were also noted in the stored sera, but the increase did not correlate to the duration of storage. Conclusions : The amount and integrity of nucleic acids extracted from stored blood samples are potential indicators that can be used for quality control. A guideline for the quality assessment of stored blood samples in a biobank is urgently needed. (Korean J Lab Med 2010;30:718-25)
Abstract
Background : One of the major concerns with biobanking is the absence of standard operating procedures to eliminate pre-analytical variation arising from sample collection, preparation, and storage. Currently, there is a lack of tools to carry out quality control procedures for stored blood samples. The aim of this study is to assess the quality of stored blood samples in our biobank and to suggest appropriate indicators for their quality control. Methods : The stored blood samples that we tested have been registered into our biobank since 2003. These were transferred to our biobank after carrying out routine requested tests, because the samples would have otherwise been discarded. For the purpose of quality control, we analyzed the concentrations and the integrity of DNA and RNA extracted from the stored samples and tested the levels of several serum proteins; the results were compared with the corresponding pre-storage levels. Results : A total of 19 samples were stored from 2006 to 2009. Of the 22 samples stored between 2003 and 2005, 50% showed complete DNA integrity. However, sufficient RNA integrity was noted in only 1 sample stored as recently as 2009. High blood urea nitrogen levels were also noted in the stored sera, but the increase did not correlate to the duration of storage. Conclusions : The amount and integrity of nucleic acids extracted from stored blood samples are potential indicators that can be used for quality control. A guideline for the quality assessment of stored blood samples in a biobank is urgently needed. (Korean J Lab Med 2010;30:718-25)
참고문헌 (28)
- "Cancer human biobank"[URL]
- "International Society for Biological and Environmental Repositories"[URL]
- "조직도"[URL]
- Elliott P, "The UK Biobank sample handling and storage protocol for the collection, processing and archiving of human blood and urine", Int J Epidemiol, 37, pp.234~244 (2008)
- Friede A, "National biospecimen network blueprint", pp.29~48 (2003), Constella Group, Inc.
- 유수진, "분변 검체의 분석 전 보관 상태가 분변잠혈반응검사 결과에 미치는 영향", The Korean Journal of Laboratory Medicine, 29(3), pp.262~267 (2009), 대한진단검사의학회
- Peakman TC, "The UK Biobank sample handling and storage validation studies", Int J Epidemiol, 37(S1), pp.i2~i6 (2008)
- Holland NT, "Biological sample collection and processing for molecular epidemiological studies", Mutat Res, 543, pp.217~234 (2003)
- Scherczinger CA, "DNA extraction from liquid blood using QIAamp", J Forensic Sci, 42, pp.893~896 (1997)
- Schroeder A, "The RIN: an RNA integrity number for assigning integrity values to RNA measurements", BMC Mol Biol, 7, pp.3~ (2006)
- Imbeaud S, "Towards standardization of RNA quality assessment using user-independent classifiers of microcapillary electrophoresis traces", Nucleic Acids Res, 33, pp.e56~ (2005)
- "인체유래검체거점은행소개"[URL]
- National Cancer Institute, "National Institute of Health, U.S. Department of Health and Human Services. National Cancer Institute best practices for biospecimen resources", National Cancer Institute, 9~10 (2007)
- International Society for Biological and Environmental Repositories, "2008 best practices for repositories: collection, storage, retrieval and distribution biological materials for research. 2nd ed", pp.23~27 (2007), International Society for Biological and Environmental Repositories
- 대한진단검사의학회, "진단검사의학", pp.9~16 (2009), 이퍼블릭
- 김순호, "진단검사의 활용", pp.135~940 (2006), 서울의학사
- 대한진단검사의학회, "진단검사의학", pp.821~824 (2009), 이퍼블릭
- Raman T, "Quality control in microarray assessment of gene expression in human airway epithelium", BMC Genomics, 10, pp.493~ (2009)
- Ribeiro-Silva A, "RNA extraction from ten year old formalin-fixed paraffin-embedded breast cancer samples: a comparison of column purification and magnetic bead-based technologies", BMC Mol Biol, 8, pp.118~ (2007)
- Strand C, "RNA quality in frozen breast cancer samples and the influence on gene expression analysis--a comparison of three evaluation methods using microcapillary electrophoresis traces", BMC Mol Biol, 8, pp.38~ (2007)
- Fajardy I, "Time course analysis of RNA stability in human placenta", BMC Mol Biol, 10, pp.21~ (2009)
- Meyer S, "Messenger RNA turnover in eukaryotes:pathways and enzymes", Crit Rev Biochem Mol Biol, 39, pp.197~216 (2004)
- Creighton TE, "Protein folding", Biochem J, 270, pp.1~16 (1990)
- Voet D, "Post-translational modification in Biochemistry", pp.1009~ (1995), John Wiley & Sons, Inc.
- Gislefoss RE, "Stability of selected serum proteins after long-term storage in the Janus Serum Bank", Clin Chem Lab Med, 47, pp.596~603 (2009)
- Boyanton BL Jr, "Stability studies of twenty-four analytes in human plasma and serum", Clin Chem, 48, pp.2242~2247 (2002)
- Zhang DJ, "Effect of serum-clot contact time on clinical chemistry laboratory results", Clin Chem, 44, pp.1325~1333 (1998)
- Tate J, "Interferences in immunoassay", Clin Biochem Rev, 25, pp.105~120 (2004)