Evaluation of Anti-dsDNA Antibody Tests: Crithidia luciliae Immunofluorescence Test, Immunoblot, Enzyme-linked Immunosorbent Assay, Chemiluminescence Immunoassay
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Background : Anti-double stranded DNA antibody (anti-dsDNA) test is useful for the diagnosis and monitoring of systemic lupus erythematosus (SLE). Although several methods are available, none of them is completely satisfactory and differences among them have been reported. We evaluated the diagnostic performance of 6 commercial kits for anti-dsDNA detection. Methods : A total of 142 sera (SLE [N=74], other systemic rheumatic diseases [N=50], other diseases [N=18]) were tested by 6 different assay kits using different antigenic sources of DNA: Crithidia luciliae immunofluorescence test (CLIFT), salmon testes (immunoblot, IB), human (ELISA I), salmon testes with nucleosome linker (ELISA II), plasmid (ELISA III), and synthetic oligonucleotides (chemiluminescence immunoassay, CLIA). Results : With manufacturers’ cut-off values, 6 test kits showed sensitivities of 55.4-91.9%. ELISA I had a greater sensitivity than the other five assays (P<0.001). The specificities of ELISA II, ELISA III, CLIA, and CLIFT were higher than those of ELISA I and IB (P<0.05). In ROC curve analysis, 3ELISA kits and CLIA showed AUC values of 0.845-0.893, and revealed no significant differences among them (P>0.05). With cut-off values set at 95% of specificity, ELISA II had a higher sensitivity than ELISA III (63.5% vs. 41.9%, P<0.05). IB had poor concordance rates with other assays (42.0-65.0%). Pearson correlation coefficients among 4 quantitative assays were 0.667-0.798. Conclusions : Six different assays showed various performances depending on the methods and cut-off values used. Except IB, the other five assays can be used for the detection of anti-dsDNA. (Korean J Lab Med 2010;30:675-84)
Abstract
Background : Anti-double stranded DNA antibody (anti-dsDNA) test is useful for the diagnosis and monitoring of systemic lupus erythematosus (SLE). Although several methods are available, none of them is completely satisfactory and differences among them have been reported. We evaluated the diagnostic performance of 6 commercial kits for anti-dsDNA detection. Methods : A total of 142 sera (SLE [N=74], other systemic rheumatic diseases [N=50], other diseases [N=18]) were tested by 6 different assay kits using different antigenic sources of DNA: Crithidia luciliae immunofluorescence test (CLIFT), salmon testes (immunoblot, IB), human (ELISA I), salmon testes with nucleosome linker (ELISA II), plasmid (ELISA III), and synthetic oligonucleotides (chemiluminescence immunoassay, CLIA). Results : With manufacturers’ cut-off values, 6 test kits showed sensitivities of 55.4-91.9%. ELISA I had a greater sensitivity than the other five assays (P<0.001). The specificities of ELISA II, ELISA III, CLIA, and CLIFT were higher than those of ELISA I and IB (P<0.05). In ROC curve analysis, 3ELISA kits and CLIA showed AUC values of 0.845-0.893, and revealed no significant differences among them (P>0.05). With cut-off values set at 95% of specificity, ELISA II had a higher sensitivity than ELISA III (63.5% vs. 41.9%, P<0.05). IB had poor concordance rates with other assays (42.0-65.0%). Pearson correlation coefficients among 4 quantitative assays were 0.667-0.798. Conclusions : Six different assays showed various performances depending on the methods and cut-off values used. Except IB, the other five assays can be used for the detection of anti-dsDNA. (Korean J Lab Med 2010;30:675-84)
참고문헌 (29)
- Kavanaugh AF, "American College of Rheumatology Ad Hoc Committee on Immunologic Testing Guidelines. Guidelines for immunologic laboratory testing in the rheumatic diseases: anti-DNA antibody tests", Arthritis Rheum, 47, pp.546~555 (2002)
- Tan EM, "The 1982 revised criteria for the classification of systemic lupus erythematosus", Arthritis Rheum, 25, pp.1271~1277 (1982)
- Reveille JD, "Predictive value of autoantibodies for activity of systemic lupus erythematosus", Lupus, 13, pp.290~297 (2004)
- Isenberg D, "Clinical laboratory assays for measuring anti-dsDNA antibodies. Where are we now?", Lupus, 11, pp.797~800 (2002)
- Sinico RA, "The use of laboratory tests in diagnosis and monitoring of systemic lupus erythematosus", J Nephrol, 15(S6), pp.S20~S27 (2002)
- Tozzoli R, "Guidelines for the laboratory use of autoantibody tests in the diagnosis and monitoring of autoimmune rheumatic diseases", Am J Clin Pathol, 117, pp.316~324 (2002)
- Emlen W, "A new ELISA for the detection of double-stranded DNA antibodies", J Immunol Methods, 132, pp.91~101 (1990)
- Radice A, "A new oligonucleotide-based ELISA for the detection of anti-double-stranded DNA antibodies", Autoimmunity, 39, pp.113~119 (2006)
- Nossent JC, "Low avidity antibodies to dsDNA as a diagnostic tool", Ann Rheum Dis, 48, pp.748~752 (1989)
- Deng JS, "Gilliam JN. The binding of antihistone antibodies to Crithidia luciliae kinetoplasts is growth cycledependent", Arthritis Rheum, 28, pp.163~168 (1985)
- Haugbro K, "AntidsDNA antibodies and disease classification in antinuclear antibody positive patients: the role of analytical diversity", Ann Rheum Dis, 63, pp.386~394 (2004)
- Sinico RA, "Interdisciplinary forum on autoimmune disease research:guidelines", G Ital Nefrol, 19, pp.212~224 (2002)
- Smeenk RJ, "Methodological update detection of antibodies to ds-DNA: current insights into its relevance", Clin Exp Rheumatol, 20, pp.294~300 (2002)
- Hylkema MN, "Clinical evaluation of a modified ELISA, using photobiotinylated DNA, for the detection of anti-DNA antibodies", J Immunol Methods, 170, pp.93~102 (1994)
- Rouquette AM, "Detection of antibodies to dsDNA:an overview of laboratory assays", Lupus, 15, pp.403~407 (2006)
- Bencivelli W, "Disease activity in systemic lupus erythematosus: report of the consensus study group of the European workshop for rheumatology research. III. Development of a computerised clinical chart and its application to the comparison of different indices of disease activity. The European Consensus Study Group for Disease Activity in SLE", Clin Exp Rheumatol, 10, pp.549~554 (1992)
- Kim KH, "Clinical significance of ELISA positive and immunofluorescence negative anti-dsDNA antibody", Clin Chim Acta, 380, pp.182~185 (2007)
- Villalta D, "Evaluation of a new automated enzyme fluoroimmunoassay using recombinant plasmid dsDNA for the detection of anti-dsDNA antibodies in SLE", J Clin Lab Anal, 16, pp.227~232 (2002)
- Antico A, "Diagnosing systemic lupus erythematosus: new-generation immunoassays for measurement of anti-dsDNA antibodies are an effective alternative to the Farr technique and the Crithidia luciliae immunofluorescence test", Lupus, 19, pp.906~912 (2010)
- Janyapoon K, "Detection of anti-dsDNA by ELISA using different sources of antigens", Pathology, 37, pp.63~68 (2005)
- Horák P, "Clinical utility of selected disease activity markers in patients with systemic lupus erythematosus", Clin Rheumatol, 20, pp.337~344 (2001)
- Wasmuth JC, "ROC analysis comparison of three assays for the detection of antibodies against double-stranded DNA in serum for the diagnosis of systemic lupus erythematosus", Clin Chem, 50, pp.2169~2171 (2004)
- Wong KH, "Measurement of anti-dsDNA: a comparative study of two ELISA and the Crithidia assay", Pathology, 30, pp.57~61 (1998)
- Tan EM, "A critical evaluation of enzyme immunoassays for detection of antinuclear autoantibodies of defined specificities. I. Precision, sensitivity, and specificity", Arthritis Rheum, 42, pp.455~464 (1999)
- Fritzler MJ, "A critical evaluation of enzyme immunoassay kits for detection of antinuclear autoantibodies of defined specificities. III. Comparative performance characteristics of academic and manufacturers’ laboratories", J Rheumatol, 30, pp.2374~2381 (2003)
- Blatt NB, "Anti-DNA autoantibodies and systemic lupus erythematosus", Pharmacol Ther, 83, pp.125~139 (1999)
- López-Longo FJ, "Simultaneous identification of various antinuclear antibodies using an automated multiparameter line immunoassay system", Lupus, 12, pp.623~629 (2003)
- 김지명, "전신 자가면역질환에서 Line Immunoassay를 이용한 항 ENA항체 및 항 dsDNA항체의 검출", The Korean Journal of Laboratory Medicine, 28(5), pp.353~361 (2008), 대한진단검사의학회
- Feltkamp TE, "The first international standard for antibodies to double stranded DNA", Ann Rheum Dis, 47, pp.740~746 (1988)