Usefulness of the recombinant liver stage antigen-3 for an early serodiagnosis of Plasmodium falciparum infection
2
조회
0
다운로드
1
인용
0
참고문헌
초록
Abstract
In order to develop tools for an early serodiagnosis of Plasmodium falciparuminfection, we evaluated theusefulness of P. falciparumliver stage antigen-3 (LSA-3) as a serodiagnostic antigen. A portion of LSA-3 gene wascloned, and its recombinant protein (rLSA-3) was expressed in Escherichia coliand purified by column chromatogra-phy. The purified rLSA-3 and 120 test blood/serum samples collected from inhabitants in malaria-endemic areas ofMandalay, Myanmar were used for this study. In microscopic examinations of blood samples, P. falciparumpositiverate was 39.1% (47/120) in thin smear trials, and 33.3% (40/120) in thick smear trials. Although the positive rate asso-ciated with the rLSA-3 (30.8%) was lower than that of the blood stage antigens (70.8%), rLSA-3 based enzyme-linkedimmunosorbent assay could detect 12 seropositive cases (10.0%), in which blood stage antigens were not detected.These results indicate that the LSA-3 is a useful antigen for an early serodiagnosis of P. falciparum infection.
참고문헌 (4)
- "Cytotoxic T-lymphocyte epitopes for HLA-B-53 and other HLA types in the malaria vaccine candidate liver stage anti-gen 3", 68, pp.227~232 (2000)
- "Chloroquine resistance in Plasmodium vivax", 48, pp.4075~4083 (2004)
- "The ears of the hippopotamus and estimates of the malaria bur-den", 64, pp.1~11 (2001)
- "Liver stage antigen-3 for serodiagnosis of falciparum malaria 53" (1994)