Comparison of Capillary Electrophoresis with Cellulose Acetate Electrophoresis for the Screening of Hemoglobinopathies
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Background: β-thalassemia is primarily found in individuals of Mediterranean and Southeast Asian ancestry. With rapid growth in the Southeast Asian segments of the Korean population, the geographic distribution of hemoglobinopathies is expected to become significantly different from what it is today. In this study, Hb fractions were measured in patients with hypochromic microcytosis to detect thalassemia and Hb variants. To evaluate the feasibility of replacing cellulose acetate electrophoresis (CA) with capillary electrophoresis (CE) in a clinical laboratory, both techniques were performed and the outcomes were compared. Methods: To evaluate hemoglobinopathies, complete blood cell counts (CBC), CA, and CE were carried out on samples from healthy and microcytic hypochromic groups. The microcytic hypochromic group consisted of 103 patients whose mean corpuscular volume (MCV) was less than 75 fL and mean corpuscular hemoglobin (MCH) was less than 24 pg. Quantitative analysis of Hb fractions was performed on 143 whole blood samples. Results: There was a good correlation for measurements of HbA (r=0.9370, P<0.0001), HbA2 (r=0.8973 P<0.0001), and HbF (r= 0.8010, P=0.0304) between the two methods. In the microcytic hypochromic group, there were 29 cases (28.2%) with decreased HbA2, 2 cases (1.9%) with increased HbA2, 3 cases (2.9%) with increased HbF, and 2 cases (1.9%) with increased HbA2 and HbF. Conclusions: CE is comparable to CA for reliable measurement of Hb fractions. It is suitable for screening of hemoglobinopathies in many clinical laboratories.
Abstract
Background: β-thalassemia is primarily found in individuals of Mediterranean and Southeast Asian ancestry. With rapid growth in the Southeast Asian segments of the Korean population, the geographic distribution of hemoglobinopathies is expected to become significantly different from what it is today. In this study, Hb fractions were measured in patients with hypochromic microcytosis to detect thalassemia and Hb variants. To evaluate the feasibility of replacing cellulose acetate electrophoresis (CA) with capillary electrophoresis (CE) in a clinical laboratory, both techniques were performed and the outcomes were compared. Methods: To evaluate hemoglobinopathies, complete blood cell counts (CBC), CA, and CE were carried out on samples from healthy and microcytic hypochromic groups. The microcytic hypochromic group consisted of 103 patients whose mean corpuscular volume (MCV) was less than 75 fL and mean corpuscular hemoglobin (MCH) was less than 24 pg. Quantitative analysis of Hb fractions was performed on 143 whole blood samples. Results: There was a good correlation for measurements of HbA (r=0.9370, P<0.0001), HbA2 (r=0.8973 P<0.0001), and HbF (r= 0.8010, P=0.0304) between the two methods. In the microcytic hypochromic group, there were 29 cases (28.2%) with decreased HbA2, 2 cases (1.9%) with increased HbA2, 3 cases (2.9%) with increased HbF, and 2 cases (1.9%) with increased HbA2 and HbF. Conclusions: CE is comparable to CA for reliable measurement of Hb fractions. It is suitable for screening of hemoglobinopathies in many clinical laboratories.
참고문헌 (23)
- Greer JP, "Wintrobe’s clinical hematology. 12th ed", pp.1083~ (2009), Lippincott Williams & Wilkins
- Olivieri NF, "The β-thalassemia", N Engl J Med, 341, pp.99~109 (1999)
- Spritz RA, "The thalassemias: molecular mechanisms of human genetic disease", Am J Hum Genet, 35, pp.333~361 (1983)
- Old J, "Haemoglobinopathies", Prenat Diagn, 16, pp.1181~1186 (1996)
- Rathod DA, "Usefulness of cell counter-based parameters and formulas in detection of β-thalassemia trait in areas of high prevalence", Am J Clin Pathol, 128, pp.585~589 (2007)
- Park SS, "Beta-thalassemia in the Korean population", Int J Hematol, 76(2), pp.93~95 (2002)
- Giambona A, "The significance of the hemoglobin A2 value in screening for hemoglobinopathies", Clin Biochem, 42, pp.1786~1796 (2009)
- Clarke GM, "Laboratory investigation of hemoglobinopathies and thalassemias: review and update", Clin Chem, 46, pp.1284~1290 (2000)
- Keren DF, "Comparison of Sebia Capillarys capillary electrophoresis with the Primus high-pressure liquid chromatography in the evaluation of hemoglobinopathies", Am J Clin Pathol, 130, pp.824~831 (2008)
- Higgins TN, "Quantification of HbA2 in patients with and without β-thalassemia and in the presence of HbS, HbC, HbE, and HbD Punjab hemoglobin variants: comparison of two systems", Am J Clin Pathol, 131, pp.357~362 (2009)
- Louahabi A, "Evaluation of a new Sebia kit for analysis of hemoglobin fractions and variants on the Capillarys system", Clin Chem Lab Med, 44, pp.340~345 (2006)
- Koo MS, "A case of β-thalassemia minor in a Korean family. The first case report in Korea", Korean J Clin Pathol, 8, pp.153~160 (1988)
- 양성은, "적혈구 소구성 저색소증 환자의 혈액학적 분석 및 고성능 액체크로마토그래피혈색소 분획 측정: β-thalassemia의 선별을 위한 시도", Annals of Laboratory Medicine, 25(3), pp.145~151 (2005), 대한진단검사의학회
- Tangvarasittichai S, "Comparison of fast protein liquid chromatography (FPLC) with HPLC, electrophoresis & microcolumn chromatography techniques for the diagnosis of β-thalassemia", Indian J Med Res, 129, pp.242~248 (2009)
- Yang Z, "Prevalence of elevated hemoglobin A2 measured by the CAPILLARYS system", Am J Clin Pathol, 131, pp.42~48 (2009)
- Kim AS, "Screening for hemoglobinopathies by complete blood cell count and hemoglobin electrophoresis", Korean J Clin Pathol, 15, pp.169~177 (1995)
- Lehmann H, "Variations in the structure of human haemoglobin. With particular reference to the unstable haemoglobins", Br Med Bull, 25, pp.14~23 (1969)
- Mais DD, "The range of hemoglobin A2 in hemoglobin E heterozygotes as determined by capillary electrophoresis", Am J Clin Pathol, 132, pp.34~38 (2009)
- McPherson RA, "Henry’s clinical diagnosis and management by laboratory methods. 21st ed", pp.521~ (2006), W.B. Saunders Company
- Schneider RG, "Identification of hemoglobins and hemoglobinopathies by electrophoresis on cellulose acetate plates impregnated with citrate agar", Clin Chem, 20, pp.74~77 (1974)
- Sirdah M, "Evaluation of the diagnostic reliability of different RBC indicies and formulas in the differentiation of the β-thalassemia minor from iron deficiency in Palestinian population", Int J Lab Hematol, 30, pp.324~330 (2008)
- McPherson RA, "Henry’s clinical diagnosis and management by laboratory methods. 21st ed", pp.532~ (2006), W.B. Saunders Company
- Young-JoonLee, "RFLP Haplotypes of β-Globin Gene Complex of -ThalassemicChromosomes in Koreans", Journal of Korean Medical Science, 17(4), pp.475~478 (2002), 대한의학회