Production of Polyclonal Antibodies against the Tegument of Sparganum (Plerocercoid of Spirometra mansoni) and Its Immunolocalization
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In a previous study, the author developed a method for separation of the tegument of spargana (plerocercoids of Spirometra mansoni) from the parenchyme using urea. The present study, as a next step, was performed to evaluate which molecules are present in the outer tegument. Two major proteins, 180 and 200 kDa, are present in the tegument and we could make polyclonal antibodies against these molecules. Their immunolocalization was processed and the outermost layer of the spargana showed strong positive staining. Conclusively, we could confirm that the 180 and 200 kDa molecules might be tightly bound membrane proteins in the tegument of spargana.
Abstract
In a previous study, the author developed a method for separation of the tegument of spargana (plerocercoids of Spirometra mansoni) from the parenchyme using urea. The present study, as a next step, was performed to evaluate which molecules are present in the outer tegument. Two major proteins, 180 and 200 kDa, are present in the tegument and we could make polyclonal antibodies against these molecules. Their immunolocalization was processed and the outermost layer of the spargana showed strong positive staining. Conclusively, we could confirm that the 180 and 200 kDa molecules might be tightly bound membrane proteins in the tegument of spargana.
참고문헌 (14)
- Chang KH, "Cerebral sparganosis: analysis of 34 cases with emphasis on CT features", Neuroradiology, 34, pp.1~8 (1992)
- Cho SY, "Some aspects of human sparganosis in Korea", Korean J Parasitol, 13, pp.60~77 (1975)
- Wilson RA, "From genomes to vaccines via the proteome", Mem Inst Oswaldo Cruz, 99, pp.45~50 (2004)
- Kim LS, "Immunohistochemical localization of 36 and 29 kDa proteins in sparganum", Korean J Parasitol, 30, pp.25~31 (1992)
- Ohnishi Y, "Antibody production in experimental sparganosis of rabbits: agglutination and indirect fluorescent antibody techniques", Jpn J Parasitol, 35, pp.25~33 (1986)
- Kim CH, "Immunohistochemical observation on the antigens inducing IgG antibodies and IgM against sparganum", Korean J Parasitol, 29, pp.339~353 (1991)
- 양현종, "Separation of the Syncytial Layer of Spargana using Urea", The Korean Journal of Parasitology, 47(1), pp.69~71 (2009), 대한기생충학회
- Mills GL, "Lipid and protein composition of the surface tegument from larvae of Taenia taeniformis", J Parasitol, 70, pp.197~207 (1984)
- Kim SI, "Protein composition and antigenicity of the tegument from Paragonimus westermani", Korean J Parasitol, 31, pp.269~276 (1993)
- Kusel JR, "Protein composition and protein synthesis in the surface membranes of Schistosoma mansoni", Parasitology, 65, pp.55~69 (1972)
- Oak JA, "Disruption and removal of the tegument from Schistosoma mansoni with Triton X-100", J Parasitol, 67, pp.761~775 (1981)
- van Balkom BW, "Mass spectrometric analysis of the Schistosoma mansoni tegumental sub-proteome", J Proteome Res, 4, pp.958~966 (2005)
- Jones MK, "The cytoskeleton and motor proteins of human schistosomes and their roles in surface maintenance and host-parasite interactions", Bioessays, 26, pp.752~765 (2004)
- Chung YB, "IgG antibody responses in early experimental sparganosis and IgG subclass responses in human sparganosis", Korean J Parasitol, 38, pp.145~150 (2000)