Utility of a Direct 16S rDNA PCR and Sequencing for Etiological Diagnosis of Infective Endocarditis
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Background: Cases of infective endocarditis (IE) require prompt etiological diagnosis for effective treatment. Molecular methods can aid in rapid and reliable diagnosis of culture-negative IE cases. We evaluated the utility of 16S rDNA PCR and sequencing in determining the causative agents of IE in valve tissues, especially when specimens were obtained after initiation of antimicrobial therapy. Methods: We performed 16S rDNA PCR and sequencing in heart valve specimens and medical records review of 80 patients who underwent protocol-based cardiac surgery from 2013 to 2015. One patient did not meet the criteria for IE. Sixty-five (81.3%) and 14 patients (17.5%) were diagnosed as having definite IE and possible IE, respectively. Blood and heart valve biopsy tissue were examined by using routine microbiological methods. Results: Blood cultures in our hospital were IE-positive for 33 patients (41.8%), whereas 49 patients (62.0%) showed positive blood cultures when initial blood cultures performed at the referring hospital were included. Eighteen (22.8%) and 40 patients (50.6%) were IE-positive in valve tissue cultures and 16S rDNA PCR, respectively. Bacteria in the Streptococcus mitis group (n=26) were the most common etiological agents of IE. Eight (10.1%) culture-negative specimens tested positive by 16S rDNA PCR. In five of eight PCR-positive and culture-negative cases, fastidious or anaerobic organisms were the cause of IE. Conclusions: Direct 16S rDNA PCR and sequencing can be used as a supplementary method to conventional blood and biopsy culture testing, especially in culture-negative IE cases that are negative for IE by culture,
Abstract
Background: Cases of infective endocarditis (IE) require prompt etiological diagnosis for effective treatment. Molecular methods can aid in rapid and reliable diagnosis of culture-negative IE cases. We evaluated the utility of 16S rDNA PCR and sequencing in determining the causative agents of IE in valve tissues, especially when specimens were obtained after initiation of antimicrobial therapy. Methods: We performed 16S rDNA PCR and sequencing in heart valve specimens and medical records review of 80 patients who underwent protocol-based cardiac surgery from 2013 to 2015. One patient did not meet the criteria for IE. Sixty-five (81.3%) and 14 patients (17.5%) were diagnosed as having definite IE and possible IE, respectively. Blood and heart valve biopsy tissue were examined by using routine microbiological methods. Results: Blood cultures in our hospital were IE-positive for 33 patients (41.8%), whereas 49 patients (62.0%) showed positive blood cultures when initial blood cultures performed at the referring hospital were included. Eighteen (22.8%) and 40 patients (50.6%) were IE-positive in valve tissue cultures and 16S rDNA PCR, respectively. Bacteria in the Streptococcus mitis group (n=26) were the most common etiological agents of IE. Eight (10.1%) culture-negative specimens tested positive by 16S rDNA PCR. In five of eight PCR-positive and culture-negative cases, fastidious or anaerobic organisms were the cause of IE. Conclusions: Direct 16S rDNA PCR and sequencing can be used as a supplementary method to conventional blood and biopsy culture testing, especially in culture-negative IE cases that are negative for IE by culture,
참고문헌 (23)
- Wouters BJ, "Segmental uniparental disomy as a recurrent mechanism for homozygous CEBPA mutations in acute myeloid leukemia", Leukemia, 21, pp.2382~2384 (2007)
- Lamas CC, "Blood culture negative endocarditis: analysis of 63 cases presenting over 25 years", Heart, 89, pp.258~262 (2003)
- Gould FK, "Guidelines for the diagnosis and antibiotic treatment of endocarditis in adults: a report of the Working Party of the British Society for Antimicrobial Chemotherapy", J Antimicrob Chemother, 67, pp.269~289 (2012)
- Nikkari S, "Yersinia-triggered reactive arthritis. Use of polymerase chain reaction and immunocytochemical staining in the detection of bacterial components from synovial specimens", Arthritis Rheum, 35, pp.682~687 (1992)
- Kotilainen P, "Diagnosis of meningococcal meningitis by broad-range bacterial PCR with cerebrospinal fluid", J Clin Microbiol, 36, pp.2205~2209 (1998)
- Rantakokko-Jalava K, "Direct amplification of rRNA genes in diagnosis of bacterial infections", J Clin Microbiol, 38, pp.32~39 (2000)
- Greub G, "Diagnosis of infectious endocarditis in patients undergoing valve surgery", Am J Med, 118, pp.230~238 (2005)
- Rovery C, "PCR detection of bacteria on cardiac valves of patients with treated bacterial endocarditis", J Clin Microbiol, 43, pp.163~167 (2005)
- Houpikian P, "Blood culture-negative endocarditis in a reference center: etiologic diagnosis of 348 cases", Medicine (Baltimore), 84, pp.162~173 (2005)
- Li JS, "Proposed modifications to the Duke criteria for the diagnosis of infective endocarditis", Clin Infect Dis, 30, pp.633~638 (2000)
- Durack DT, "New criteria for diagnosis of infective endocarditis: utilization of specific echocardiographic findings. Duke Endocarditis Service", Am J Med, 96, pp.200~209 (1994)
- Universal bacterial 16S rDNA amplification and sequencing. Diagnostic molecular microbiology: principles and applications, "Relman DA", American Society for Microbiology, 489~495 (1993)
- Choi SH, "Usefulness of a direct 16S rRNA gene PCR assay of percutaneous biopsies or aspirates for etiological diagnosis of vertebral osteomyelitis", Diagn Microbiol Infect Dis, 78, pp.75~78 (2014)
- CLSI, "Interpretive criteria for identification of bacteria and fungi by dna target sequencing; Approved guideline MM18-A", CLSI, ~ (2008)
- Kim OS, "Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species", Int J Syst Evol Microbiol, 62, pp.716~721 (2012)
- Park KS, "Evaluation of the GenBank, EzTaxon, and BIBI services for molecular identification of clinical blood culture isolates that were unidentifiable or misidentified by conventional methods", J Clin Microbiol, 50, pp.1792~1795 (2012)
- Pant S, "Trends in infective endocarditis incidence, microbiology, and valve replacement in the United States from 2000 to 2011", J Am Coll Cardiol, 65, pp.2070~2076 (2015)
- Miller RJ, "Development and evaluation of a novel fast broad-range 16S ribosomal DNA PCR and sequencing assay for diagnosis of bacterial infective endocarditis: multi-year experience in a large Canadian healthcare zone and a literature review", BMC Infect Dis, 16, pp.146~ (2016)
- Kotilainen P, "Aetiological diagnosis of infective endocarditis by direct amplification of rRNA genes from surgically removed valve tissue. An 11-year experience in a Finnish teaching hospital", Ann Med, 38, pp.263~273 (2006)
- Fournier PE, "Infective endocarditis: epidemiology, diagnosis, imaging, therapy, and prevention", pp.245~258 (2016), Springer International Publishing AG
- Horstkotte D, "Guidelines on prevention, diagnosis and treatment of infective endocarditis executive summary; the task force on infective endocarditis of the European society of cardiology", Eur Heart J, 25, pp.267~276 (2004)
- "Task Force sull’Endocardite Infettiva della Società Europea di Cardiologia. Guidelines on prevention, diagnosis and treatment of infective endocarditis", Ital Heart J Suppl, 5, pp.548~590 (2004)
- Morris AJ, "Clinical impact of bacteria and fungi recovered only from broth cultures", J Clin Microbiol, 33, pp.161~165 (1995)