Detection of Tick-Borne Pathogens in the Korean Water Deer (Hydropotes inermis argyropus) from Jeonbuk Province, Korea
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The objective of this study was to investigate the prevalence of tick-borne pathogens in the Korean water deer (Hydropotes inermis argyropus). Pathogens were identified using PCR which included Anaplasma, Ehrlichia, Rickettsia, and Theileria. Rickettsia was not detected, whereas Anaplasma, Ehrlichia, and Theileria infections were detected in 4, 2, and 8 animals, respectively. The most prevalent pathogen was Theileria. Of the 8 Theileria-positive animals, 2 were mixed-infected with 3 pathogens (Anaplasma, Ehrlichia, and Theileria) and another 2 animals showed mixed-infection with 2 pathogens (Anaplasma and Theileria). Sequencing analysis was used to verify the PCR results. The pathogens found in this study were identified as Anaplasma phagocytophilum, Ehrlichia canis, and Theileria sp. To the best of our knowledge, this is the first report identifying these 3 pathogens in the Korean water deer. Our results suggest that the Korean water deer may serve as a major reservoir for these tick-borne pathogens, leading to spread of tick-borne diseases to domestic animals, livestock, and humans. Further studies are needed to investigate their roles in this respect.
Abstract
The objective of this study was to investigate the prevalence of tick-borne pathogens in the Korean water deer (Hydropotes inermis argyropus). Pathogens were identified using PCR which included Anaplasma, Ehrlichia, Rickettsia, and Theileria. Rickettsia was not detected, whereas Anaplasma, Ehrlichia, and Theileria infections were detected in 4, 2, and 8 animals, respectively. The most prevalent pathogen was Theileria. Of the 8 Theileria-positive animals, 2 were mixed-infected with 3 pathogens (Anaplasma, Ehrlichia, and Theileria) and another 2 animals showed mixed-infection with 2 pathogens (Anaplasma and Theileria). Sequencing analysis was used to verify the PCR results. The pathogens found in this study were identified as Anaplasma phagocytophilum, Ehrlichia canis, and Theileria sp. To the best of our knowledge, this is the first report identifying these 3 pathogens in the Korean water deer. Our results suggest that the Korean water deer may serve as a major reservoir for these tick-borne pathogens, leading to spread of tick-borne diseases to domestic animals, livestock, and humans. Further studies are needed to investigate their roles in this respect.
참고문헌 (23)
- Taylor MA, "Emerging parasitic diseases of sheep", Vet Parasitol, 189, pp.2~7 (2012)
- Dantas-Torres F, "Ticks and tick-borne diseases: a one health perspective", Trends Parasitol, 28, pp.437~446 (2012)
- Uilenberg G, "Importance of ticks and tick-borne diseases of domestic ruminants in Southern Europe", Parassitologia, 41, pp.107~109 (1999)
- Eygelaar D, "Tick-borne haemoparasites in African buffalo (Syncerus caffer) from two wildlife areas in Northern Botswana", Parasit Vectors, 8, pp.26~ (2015)
- Kang JG, "New genetic variants of Anaplasma phagocytophilum and Anaplasma bovis from Korean water deer (Hydropotes inermis argyropus)", Vector Borne Zoon Dis, 11, pp.929~938 (2011)
- Ko S, "Molecular detection of Bartonella grahamii and B. schoenbuchensis-related species in Korean water deer (Hydropotes inermis argyropus)", Vector Borne Zoon Dis, 13, pp.415~418 (2013)
- Shin GW, "The prevalence of Coxiella burnetii infection in wild Korean water deer, Korea", J Vet Med Sci, 76, pp.1069~1071 (2014)
- Harris RB, "Hydropotes inermis. In: IUCN 2010. IUCN Red List of Threatened Species. Version 2010.1", ~ (2008)
- Kim BJ, "Distribution, density, and habitat use of the Korean water deer (Hydropotes inermis argyropus) in Korea", Landscape Ecol Eng, 7, pp.291~297 (2011)
- Thompson JD, "The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools", Nucleic Acids Res, 25, pp.4876~4882 (1997)
- Saitou N, "The neighbor-joining method: a new method for reconstructing phylogenetic trees", Mol Biol Evol, 4, pp.406~425 (1987)
- Page RD, "TreeView: an application to display phylogenetic trees on personal computers", Comp Appl Biosci, 12, pp.357~358 (1996)
- Han JI, "High prevalence of Theileria sp. in wild Chinese water deer (Hydropotes inermis argyropus) in South Korea", Vet Parasitol, 164, pp.311~314 (2009)
- Dumler JS, "Human granulocytic anaplasmosis and Anaplasma phagocytophilum", Emerg Infect Dis, 11, pp.1828~1834 (2005)
- Santos AS, "Human exposure to Anaplasma phagocytophilum in Portugal", Ann N Y Acad Sci, 1078, pp.100~105 (2006)
- Stuen S, "Anaplasma phagocytophilum--a widespread multi-host pathogen with highly adaptive strategies", Front Cell Infect Microbiol, 3, pp.31~ (2013)
- Perez M, "Ehrlichia canis-like agent isolated from a man in Venezuela: antigenic and genetic characterization", J Clin Microbiol, 34, pp.2133~2139 (1996)
- Aktas M, "A survey of ixodid tick species and molecular identification of tick-borne pathogens", Vet Parasitol, 200, pp.276~283 (2014)
- Ebani VV, "Serological survey of Borrelia burgdorferi sensu lato, Anaplasma phagocytophilum, and Ehrlichia canis infections in rural and urban dogs in Central Italy", Ann Agric Environ Med, 21, pp.671~675 (2014)
- Andre MR, "Molecular detection of tick-borne bacterial agents in Brazilian and exotic captive carnivores", Ticks Tick Borne Dis, 3, pp.247~253 (2012)
- Tateno M, "Molecular epidemiologic survey of Bartonella, Ehrlichia, and Anaplasma infections in Japanese Iriomote and Tsushima leopard cats", J Wildl Dis, 49, pp.646~652 (2013)
- Torina A, "A molecular survey of Anaplasma spp., Rickettsia spp., Ehrlichia canis and Babesia microti in foxes and fleas from Sicily", Transbound Emerg Dis, 60, pp.125~130 (2013)
- Luo J, "Theileriosis of sheep and goats in China", Trop Anim Health Prod, 29, pp.8S~10S (1997)