Influence of 120 kDa Pyruvate:Ferredoxin Oxidoreductase on Pathogenicity of Trichomonas vaginalis
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Trichomonas vaginalis is a flagellate protozoan parasite and commonly infected the lower genital tract in women and men. Iron is a known nutrient for growth of various pathogens, and also reported to be involved in establishment of trichomoniasis. However, the exact mechanism was not clarified. In this study, the author investigated whether the 120 kDa protein of T. vaginalis may be involved in pathogenicity of trichomonads. Antibodies against 120 kDa protein of T. vaginalis, which was identified as pyruvate:ferredoxin oxidoreductase (PFOR) by peptide analysis of MALDI-TOF-MS, were prepared in rabbits. Pretreatment of T. vaginalis with anti-120 kDa Ab decreased the proliferation and adherence to vaginal epithelial cells (MS74) of T. vaginalis. Subcutaneous tissue abscess in anti-120 kDa Ab-treated T. vaginalis-injected mice was smaller in size than that of untreated T. vaginalis-infected mice. Collectively, the 120 kDa protein expressed by iron may be involved in proliferation, adhesion to host cells, and abscess formation, thereby may influence on the pathogenicity of T. vaginalis.
Abstract
Trichomonas vaginalis is a flagellate protozoan parasite and commonly infected the lower genital tract in women and men. Iron is a known nutrient for growth of various pathogens, and also reported to be involved in establishment of trichomoniasis. However, the exact mechanism was not clarified. In this study, the author investigated whether the 120 kDa protein of T. vaginalis may be involved in pathogenicity of trichomonads. Antibodies against 120 kDa protein of T. vaginalis, which was identified as pyruvate:ferredoxin oxidoreductase (PFOR) by peptide analysis of MALDI-TOF-MS, were prepared in rabbits. Pretreatment of T. vaginalis with anti-120 kDa Ab decreased the proliferation and adherence to vaginal epithelial cells (MS74) of T. vaginalis. Subcutaneous tissue abscess in anti-120 kDa Ab-treated T. vaginalis-injected mice was smaller in size than that of untreated T. vaginalis-infected mice. Collectively, the 120 kDa protein expressed by iron may be involved in proliferation, adhesion to host cells, and abscess formation, thereby may influence on the pathogenicity of T. vaginalis.
참고문헌 (16)
- Gorrell TE, "Effects of culture medium content on the biochemical composition and metabolism of Trichomonas vaginalis", J Bacteriol, 161, pp.1228~1230 (1985)
- Ryu JS, "Effect of iron on the virulence of Trichomonas vaginalis", J Parasitol, 87, pp.457~460 (2001)
- Moreno-Brito V, "A Trichomonas vaginalis 120 kDa protein with identity to hydrogenosome pyruvate : ferredoxin oxidoreductase is a surface adhesin induced by iron", Cell Microbiol, 7, pp.245~258 (2005)
- Meza-Cervantez P, "Pyruvate:ferredoxin oxidoreductase (PFO) is a surface-associated cell-binding protein in Trichomonas vaginalis and is involved in trichomonal adherence to host cells", Microbiology, 157(Pt 12), pp.3469~3482 (2011)
- Diamond LS, "The establishment of various trichomonads of animals and man in axenic cultures", J Parasitol, 43, pp.488~490 (1957)
- Lehker MW, "The regulation by iron of the synthesis of adhesins and cytoadherence levels in the protozoan Trichomonas vaginalis", J Exp Med, 174, pp.311~318 (1991)
- Weinberg ED, "The role of iron in protozoan and fungal infectious diseases", J Eukaryot Microbiol, 46, pp.231~238 (1999)
- Lehker MW, "Iron regulates growth of Trichomonas vaginalis and the expression of immunogenic trichomonad proteins", Mol Microbiol, 6, pp.123~132 (1992)
- Griffith E, "In Iron and Infection: Molecular, Physiological and Clinical Aspects", pp.1~25 (1985), John Wiley and Sons
- Tachezy J, "Tritrichomonas foetus: iron acquisition from lactoferrin and transferrin", Exp Parasitol, 83, pp.216~228 (1996)
- Beaver PC, "Clinical Parasitology", pp.49~52 (1984), Lea & Febiger
- 김용석, "Hydrogenosomal activity of Trichomonas vaginalis cultivated under different iron conditions", The Korean Journal of Parasitology, 44(4), pp.373~378 (2006), 대한기생충학ㆍ열대의학회
- Alderete JF, "The mechanisms and molecules involved in cytoadherence and pathogenesis of Trichomonas vaginalis", Parasitol Today, 11, pp.70~74 (1995)
- Krieger JN, "Contact-dependent cytopathogenic mechanism of Trichomonas vaginalis", Infect Immun, 50, pp.778~786 (1985)
- Mendoza-Lopez MR, "CP30, a cysteine proteinase involved in Trichomonas vaginalis cytoadherence", Infect Immun, 68, pp.4907~4912 (2000)
- Demes P, "Fewer Trichomonas vaginalis organisms in vaginas of infected women during menstruation", Genitourin Med, 64, pp.22~24 (1988)