Differential Effects of Two Widely Used Solvents, DMSO and Ethanol, on the Growth and Recovery of Trypanosoma cruzi Epimastigotes in Culture
0
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
Trypanosoma cruzi is the etiological agent of Chagas disease. Epimastigote forms of T. cruzi can be readily cultured in axenic conditions. Ethanol and dimethyl sulfoxide (DMSO) are commonly used solvents employed as vehicles for hydrophobic compounds. In order to produce a reference plot of solvent dependent growth inhibition for T. cruzi research, the growth of epimastigotes was analyzed in the presence of different concentrations of ethanol (0.1-4.0%) and DMSO (0.5-7.5%). The ability of the parasites to resume growth after removal of these solvents was also examined. As expected, both ethanol and DMSO produced a dose-dependent inhibition of cellular growth. Parasites could recover normal growth after 9 days in up to 2% ethanol or 5% DMSO. Since DMSO was better tolerated than ethanol, it is thus recommended to prefer DMSO over ethanol in the case of a similar solubility of a given compound.
Abstract
Trypanosoma cruzi is the etiological agent of Chagas disease. Epimastigote forms of T. cruzi can be readily cultured in axenic conditions. Ethanol and dimethyl sulfoxide (DMSO) are commonly used solvents employed as vehicles for hydrophobic compounds. In order to produce a reference plot of solvent dependent growth inhibition for T. cruzi research, the growth of epimastigotes was analyzed in the presence of different concentrations of ethanol (0.1-4.0%) and DMSO (0.5-7.5%). The ability of the parasites to resume growth after removal of these solvents was also examined. As expected, both ethanol and DMSO produced a dose-dependent inhibition of cellular growth. Parasites could recover normal growth after 9 days in up to 2% ethanol or 5% DMSO. Since DMSO was better tolerated than ethanol, it is thus recommended to prefer DMSO over ethanol in the case of a similar solubility of a given compound.
참고문헌 (20)
- Köberle F, "Chagas' disease and Chagas' syndromes: the pathology of American trypanosomiasis", Adv Parasitol, 6, pp.63~116 (1968)
- Weiss LM, "Preface to Chagas disease", Adv Parasitol, 76, pp.21~26 (2011)
- Bern C, "Evaluation and treatment of Chagas disease in the United States : a systematic reFigview", JAMA, 298, pp.2171~2181 (2007)
- Díaz-Urrutia CA, "Biological and chemical study of fused tri-and tetracyclic indazoles and analogues with important antiparasitic activity", Spectrochim Acta A Mol Biomol Spectrosc, 95, pp.670~678 (2012)
- Vigueira PA, "Effects of the green tea catechin()-epigallocatechin gallate on Trypanosoma brucei", Int J Parasitol Drugs Drug Resist, 2, pp.225~229 (2012)
- Lim KT, "Development of resazurin-based assay in 384-well format for high throughput whole cell screening of Trypanosoma brucei rhodesiense strain STIB 900 for the identification of potential anti-trypanosomal agents", Exp Parasitol, 162, pp.49~56 (2016)
- Simões-Silva MR, "Phenotypic screening in vitro of novel aromatic amidines against Trypanosoma cruzi", Antimicrob Agents Chemother, 60, pp.4701~4707 (2016)
- Tyler KM, "The life cycle of Trypanosoma cruzi revisited", Int J Parasitol, 31, pp.472~481 (2001)
- El Jay A, "Toxic effects of organic solvents on the growth of Chlorella vulgaris and Selenastrum capricornutum", Bull Environ Contam Toxicol, 57, pp.191~198 (1996)
- Galvao J, "Unexpected low-dose toxicity of the universal solvent DMSO", FASEB J, 28, pp.1317~1330 (2014)
- Gerhards E, "The metabolism of dimethyl sulfoxide and its metabolic effects in man and animals", Ann N Y Acad Sci, 141, pp.65~76 (1967)
- Teschke R, "Hepatic microsomal alcohol-oxidizing system. Affinity for methanol, ethanol, propanol, and butanol", J Biol Chem, 250, pp.7397~7404 (1975)
- Camargo EP, "Growth and differentiation in Trypanosoma cruzi. I. Origin of metacyclic trypanosomes in liquid media", Rev Inst Med Trop Sao Paulo, 6, pp.93~100 (1964)
- Jacob SW, "Pharmacology of DMSO", Cryobiology, 23, pp.14~27 (1986)
- Harris RA, "Ethanol's molecular targets", Sci Signal, 1, pp.re7~ (2008)
- Jones RP, "Biological principles for the effects of ethanol", Enzyme Microb Technol, 11, pp.130~153 (1989)
- Friend C, "Hemoglobin synthesis in murine virus-induced leukemic cells in vitro : stimulation of erythroid differentiation by dimethyl sulfoxide", Proc Natl Acad Sci USA, 68, pp.378~382 (1971)
- Kluge N, "Dimethylsulfoxide-induced differentiation and hemoglobin synthesis in tissue cultures of rat erythroleukemia cells transformed by 7,12-dimethylbenz(a)anthracene", Proc Natl Acad Sci USA, 73, pp.1237~1240 (1976)
- Collins SJ, "Terminal differentiation of human promyelocytic leukemia cells induced by dimethyl sulfoxide and other polar compounds", Proc Natl Acad Sci USA, 75, pp.2458~2462 (1978)
- Basch H, "In vitro antimicrobial activity of dimethylsulfoxide", Appl Microbiol, 16, pp.1953~1954 (1968)