DNA Methylation of Gene Expression in Acanthamoeba castellanii Encystation
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초록
Encystation mediating cyst specific cysteine proteinase (CSCP) of Acanthamoeba castellanii is expressed remarkably during encystation. However, the molecular mechanism involved in the regulation of CSCP gene expression remains unclear. In this study, we focused on epigenetic regulation of gene expression during encystation of Acanthamoeba. To evaluate methylation as a potential mechanism involved in the regulation of CSCP expression, we first investigated the correlation between promoter methylation status of CSCP gene and its expression. A 2,878 bp of promoter sequence of CSCP gene was amplified by PCR. Three CpG islands (island 1-3) were detected in this sequence using bioinformatics tools. Methylation of CpG island in trophozoites and cysts was measured by bisulfite sequence PCR. CSCP promoter methylation of CpG island 1 (1,633 bp) was found in 8.2% of trophozoites and 7.3% of cysts. Methylation of CpG island 2 (625 bp) was observed in 4.2% of trophozoites and 5.8% of cysts. Methylation of CpG island 3 (367 bp) in trophozoites and cysts was both 3.6%. These results suggest that DNA methylation system is present in CSCP gene expression of Acanthamoeba. In addition, the expression of encystation mediating CSCP is correlated with promoter CpG island 1 hypomethylation.
Abstract
Encystation mediating cyst specific cysteine proteinase (CSCP) of Acanthamoeba castellanii is expressed remarkably during encystation. However, the molecular mechanism involved in the regulation of CSCP gene expression remains unclear. In this study, we focused on epigenetic regulation of gene expression during encystation of Acanthamoeba. To evaluate methylation as a potential mechanism involved in the regulation of CSCP expression, we first investigated the correlation between promoter methylation status of CSCP gene and its expression. A 2,878 bp of promoter sequence of CSCP gene was amplified by PCR. Three CpG islands (island 1-3) were detected in this sequence using bioinformatics tools. Methylation of CpG island in trophozoites and cysts was measured by bisulfite sequence PCR. CSCP promoter methylation of CpG island 1 (1,633 bp) was found in 8.2% of trophozoites and 7.3% of cysts. Methylation of CpG island 2 (625 bp) was observed in 4.2% of trophozoites and 5.8% of cysts. Methylation of CpG island 3 (367 bp) in trophozoites and cysts was both 3.6%. These results suggest that DNA methylation system is present in CSCP gene expression of Acanthamoeba. In addition, the expression of encystation mediating CSCP is correlated with promoter CpG island 1 hypomethylation.
참고문헌 (19)
- Aksozek A, "Resistance of Acanthamoeba castellanii cysts to physical, chemical, and radiological conditions", J Parasitol, 88, pp.621~623 (2002)
- Turner NA, "Emergence of resistance to biocides during differentiation of Acanthamoeba castellanii", J Antimicrob Chemother, 46, pp.27~34 (2000)
- 문은경, "Microarray Analysis of Differentially Expressed Genes between Cysts and Trophozoites of Acanthamoeba castellanii", The Korean Journal of Parasitology, 49(4), pp.341~347 (2011), 대한기생충학ㆍ열대의학회
- Moon EK, "Autophagy protein 8 mediating autophagosome in encysting Acanthamoeba", Mol Biochem Parasitol, 168, pp.43~48 (2009)
- Song SM, "Autophagy protein 16-mediated autophagy is required for the encystation of Acanthamoeba castellanii", Mol Biochem Parasitol, 183, pp.158~165 (2012)
- Eun-Kyung Moon, "Down-regulation of cellulose synthase inhibits the formation of endocysts in Acanthamoeba", The Korean Journal of Parasitology, 52(2), pp.131~135 (2014), 대한기생충학ㆍ열대의학회[DOI]
- Moon EK, "Characterization of a serine proteinase mediating encystation of Acanthamoeba", Eukaryot Cell, 7, pp.1513~1517 (2008)
- Moon EK, "Cysteine protease involving in autophagosomal degradation of mitochondria during encystation of Acanthamoeba", Mol Biochem Parasitol, 185, pp.121~126 (2012)
- Portela A, "Epigenetic modifications and human disease", Nat Biotechnol, 28, pp.1057~1068 (2010)
- Robertson KD, "DNA methylation and chromatin-unraveling the tangled web", Oncogene, 21, pp.5361~5379 (2002)
- Attwood JT, "DNA methylation and the regulation of gene transcription", Cell Mol Life Sci, 59, pp.241~257 (2002)
- Goto T, "Regulation of X-chromosome inactivation in development in mice and humans", Microbiol Mol Biol Rev, 62, pp.362~378 (1998)
- Fisher O, "Characterization of cytosine methylated regions and 5-cytosine DNA methyltransferase(Ehmeth)in the protozoan parasite Entamoeba histolytica", Nucleic Acids Res, 32, pp.287~297 (2004)
- Ponts N, "Genome-wide mapping of DNA methylation in the human malaria parasite Plasmodium falciparum", Cell Host Microbe, 14, pp.696~706 (2013)
- Geyer KK, "Cytosine methylation regulates oviposition in the pathogenic blood fluke Schistosoma mansoni", Nat Commun, 2, pp.424~ (2011)
- Eun-Kyung Moon, "Identification of Protein Arginine Methyltransferase 5 as a Regulator for Encystation of Acanthamoeba", The Korean Journal of Parasitology, 54(2), pp.133~138 (2016), 대한기생충학ㆍ열대의학회
- Bernes S, "Epigenetic and classical activation of Entamoeba histolytica heat shock protein 100(EHsp100)expression", FEBS Lett, 579, pp.6395~6402 (2005)
- Jones PA, "Rethinking how DNA methylation patterns are maintained", Nat Rev Genet, 10, pp.805~811 (2009)
- Lim JH, "Activation of human cancer", testis antigen gene, XAGE-1, in tumor cells is correlated with CpG island hypomethylation, 2005, pp.116, 200~206