Phylogenetic Positioning of a Strongyloides stercoralis Isolate Recovered from a Korean Patient and Comparison with Other Asian Isolates
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Strongyloidiasis is caused by Strongyloides stercoralis and is one of the most neglected tropical diseases in tropical and subtropical regions. Although several strongyloidiasis cases have been reported in Korea, genetic analysis of Korean isolates is still incomplete. In this study, a parasite was isolated from a 61-year-old man diagnosed with strongy- loidiasis during the treatment of lymphoma on his retroperitoneal lymph node. Diffuse symmetric wall thickening from the ascending to descending colon and a nematode-infected intestine was observed following microscopic examination. Ge- nomic DNA was isolated from a patient tissue block, and S. stercoralis was identified by PCR and sequencing (18S rDNA). In order to determine phylogenetic location of a Korean isolate (named KS1), we analyzed cox1 gene (500-bp) and compared it with that from 47 previous S. stercoralis isolates (28 human isolates and 19 canid isolates) from Asian coun- tries. Our results showed that phylogenetic tree could clearly be divided into 5 different groups according to hosts and re- gions. KS1 was most closely related with the Chinese isolates in terms of genetic distance.
Abstract
Strongyloidiasis is caused by Strongyloides stercoralis and is one of the most neglected tropical diseases in tropical and subtropical regions. Although several strongyloidiasis cases have been reported in Korea, genetic analysis of Korean isolates is still incomplete. In this study, a parasite was isolated from a 61-year-old man diagnosed with strongy- loidiasis during the treatment of lymphoma on his retroperitoneal lymph node. Diffuse symmetric wall thickening from the ascending to descending colon and a nematode-infected intestine was observed following microscopic examination. Ge- nomic DNA was isolated from a patient tissue block, and S. stercoralis was identified by PCR and sequencing (18S rDNA). In order to determine phylogenetic location of a Korean isolate (named KS1), we analyzed cox1 gene (500-bp) and compared it with that from 47 previous S. stercoralis isolates (28 human isolates and 19 canid isolates) from Asian coun- tries. Our results showed that phylogenetic tree could clearly be divided into 5 different groups according to hosts and re- gions. KS1 was most closely related with the Chinese isolates in terms of genetic distance.
참고문헌 (19)
- Krolewiecki A, "Strongyloidiasis : a Neglected Tropical Disease", Infect Dis Clin North Am, 33, pp.135~151 (2019)[DOI]
- Grove DI, "Human strongyloidiasis", Adv Parasitol, 38, pp.251~309 (1996)[DOI]
- Beknazarova M, "Strongyloidiasis : a disease of socioeconomic disadvantage", Int J Environ Res Public Health, 13, pp.517~ (2016)[DOI]
- 서안나, "Comorbid Gastric Adenocarcinoma and Gastric and Duodenal Strongyloides stercoralis Infection: A Case Report", Parasites, Hosts and Diseases, 53(1), pp.95~99 (2015), 대한기생충학ㆍ열대의학회
- Hannah Ra, "Strongyloidiasis Presenting as yellowish nodules in Colonoscopy of an Immunocompetent Patient", Clinical Endoscopy, 52(1), pp.80~82 (2019), 대한소화기내시경학회[DOI]
- Hakeem I, "Strongyloidiasis hyperinfection after renal transplant presenting as diffuse alveolar hemorrhage with respiratory failure", Proc (Bayl Univ Med Cent), 32, pp.413~416 (2019)[DOI]
- Ronan SG, "Disseminated strongyloidiasis presenting as purpura", J Am Acad Dermatol, 21, pp.1123~1125 (1989)[DOI]
- Waters M, "Case report : two cases of strongyloidiasis presenting with thrombotic events", Am J Trop Med Hyg, 101, pp.418~421 (2019)[DOI]
- Spotin A, "Assessment of the global paradigms of genetic variability in Strongyloides stercoralis infrapopulations determined by mitochondrial DNA sequences", Comp Immunol Microbiol Infect Dis, 67, pp.101354~ (2019)[DOI]
- Nagayasu E, "A possible origin population of pathogenic intestinal nematodes, Strongyloides stercoralis, unveiled by molecular phylogeny", Sci Rep, 7, pp.4844~ (2017)[DOI]
- Ko PP, "Phylogenetic relationships of Strongyloides species in carnivore hosts", Parasitol Int, 78, pp.102151~ (2020)[DOI]
- Barratt JLN, "A global genotyping survey of Strongyloides stercoralis and Strongyloides fuelleborni using deep amplicon sequencing", PLoS Negl Trop Dis, 13, pp.e0007609~ (2019)[DOI]
- 김은진, "Acute Respiratory Distress Syndrome With Alveolar Hemorrhage due to Strongyloidiasis Hyperinfection in an Older Patient", Annals of geriatric medicine and research, 22(4), pp.200~203 (2018), 대한노인병학회[DOI]
- Eun Jeong Won, "Strongyloidiasis in a Diabetic Patient Accompanied by Gastrointestinal Stromal Tumor: Cause of Eosinophilia Unresponsive to Steroid Therapy", Parasites, Hosts and Diseases, 53(2), pp.223~226 (2015), 대한기생충학ㆍ열대의학회
- Sitta RB, "Conventional PCR for molecular diagnosis of human strongyloidiasis", Parasitology, 141, pp.716~721 (2014)[DOI]
- Moghaddassani H, "Molecular Diagnosis of Strongyloides stercoralis Infection by PCR Detection of Specific DNA in Human Stool Samples", Iran J Parasitol, 6, pp.23~30 (2011)
- Sharifdini M, "Comparison of Nested Polymerase Chain Reaction and Real-Time Polymerase Chain Reaction with Parasitological Methods for Detection of Strongyloides stercoralis in Human Fecal Samples", Am J Trop Med Hyg, 93, pp.1285~1291 (2015)[DOI]
- 김진, "A case of fatal hyperinfective strongyloidiasis with discovery of autoinfective filariform larvae in sputum", Parasites, Hosts and Diseases, 43(2), pp.1~16 (2005), 대한기생충학ㆍ열대의학회
- Jaleta TG, "Different but overlapping populations of Strongyloides stercoralis in dogs and humans-Dogs as a possible source for zoonotic strongyloidiasis", PLoS Negl Trop Dis, 11, pp.e0005752~ (2017)[DOI]